LOCUS       sequence-345971-        8816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_Ollas.S.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2217..2443
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2444..2624
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2671..2796
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3289..3522
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4049..4297
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4049..4069
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4220..4239
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4316..4391
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4439..4556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4696..5873
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4927..5864
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5821..5841
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5922..5931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5934..5975
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             5997..6041
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6060..6101
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6111..6818
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6256..6274)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6524..6543
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6888..7476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6941..6961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7359..7370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7551..7784
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7856..7977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7893..7912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7947..7966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8017..8152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8172..8191)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8173..8191)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8201..8218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8201..8217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8210..8232)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8359..8814
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8446..8465)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8656..8677
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     2221 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     2281 acaaggagag aaaaagcacc gtgcatggcg attggtggaa gtaaggtggt acgatcgtgc
     2341 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     2401 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     2461 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     2521 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     2581 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     2641 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     2701 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     2761 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     2821 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     2881 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     2941 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     3001 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     3061 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     3121 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     3181 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     3241 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     3301 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     3361 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     3421 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     3481 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     3541 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     3601 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
     4021 ctagtatcga gcggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt
     4081 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     4141 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     4201 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     4261 cttggcttta tatatcttgt ggaaaggacg aaacaccggg tcttcgagaa gacctgtttt
     4321 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
     4381 cgagtcggtg ctttttttaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     4441 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4501 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     4561 tttattacag ggacagcaga gatccacttt ggccgcggct cgaggcgata agctttgcaa
     4621 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     4681 aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     4741 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     4801 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     4861 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     4921 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     4981 tgcgtgcctt gaattacttc cactggctgc agtacgtgat tcttgatccc gagcttcggg
     5041 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
     5101 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
     5161 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
     5221 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
     5281 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
     5341 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
     5401 ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
     5461 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
     5521 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
     5581 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt
     5641 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
     5701 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
     5761 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
     5821 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaattc
     5881 tgcagtcgat cgacggtacc gcgggcccgg gatccacggt cgccaccatg ggcagcggct
     5941 tcgccaacga gctgggcccc aggctgatgg gcaagtgcgg aggaggcggg ggagggaagg
     6001 agaccgccgc cgccaagttc gagaggcagc acatggacag cggaggaggc gggggagggg
     6061 gcaaaccaat tccaaaccca ctgctgggac tggattccac aggcatgctg atggtgagca
     6121 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     6181 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     6241 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     6301 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     6361 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     6421 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     6481 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     6541 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     6601 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6661 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6721 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6781 gccgccactc caccggcggc atggacgagc tgtacaagaa acggccagcc gaggatatgg
     6841 aggaagaaca agcctttaaa cgatctcgat aaagcggccg cgtcgacaat caacctctgg
     6901 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     6961 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7021 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7081 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7141 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7201 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7261 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     7321 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7381 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc
     7441 agacgagtcg gatctccctt tgggccgcct ccccgcctgg gtacctttaa gaccaatgac
     7501 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7561 aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc tctggttaga
     7621 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7681 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7741 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     7801 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7861 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7921 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7981 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8041 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8101 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     8161 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     8221 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     8281 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     8341 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     8401 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     8461 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     8521 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     8581 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8641 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8701 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8761 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttag
//
