LOCUS       sequence-34608-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid; This plasmid is the backbone for 
            the DECIPHER libraries (currently DISCONTINUED).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Frangou, G
  TITLE     DECIPHER library reagents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..142
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    327..444
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
       61 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      121 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      181 tgtggaaagg acgaaacacc ggagtcttct tttttgaaga cacttcgtgc gtttggggtt
      241 tcggactgta gaactctgaa cctctcggtg gtcgccgtat cattagaatt ctcgacctcg
      301 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
      361 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
      421 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg
      481 ccgccgttcg aaggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
      541 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
      601 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
      661 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
      721 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
      781 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt cc
//
