LOCUS       sequence-34609-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral plasmid; This plasmid is the backbone for 
            the DECIPHER libraries (currently DISCONTINUED).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Frangou, G
  TITLE     DECIPHER library reagents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..744)
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     regulatory      741..750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcatgttaga aggcttcctc tgccctcgga tccattaagt ttgtgcccca gtttgctagg
       61 gaggtcgcag tatctggcca cagccacctc gtgctgctcg acgtaggtct ctttgtcggc
      121 ctccttgatt ctttccagtc tgtggtccac atagtagacg ccgggcatct tgaggttctt
      181 agcgggtttc ttggatctgt atgtggtctt gaagttgcag atcaggtggc ccccgcccac
      241 gagcttcagg gccatgtcgc ttctgccttc caggccgccg tcagcggggt acagcatctc
      301 ggtgttggcc tcccagccga gtgttttctt ctgcatcaca gggccgttgg atgggaagtt
      361 cacccctctg atcttgacgt tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt
      421 agcggtcagc acgcccccgt cctcgtatgt ggtgactctc tcccatgtga agccctcagg
      481 gaaggactgc ttaaagaagt cggggatgcc ctgggtgtgg ttgatgaagg ttctgctgcc
      541 gtacatgaag ctggtagcca ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac
      601 cttgattctc atggtctggg tgccctcgta gggcttgcct tcgccctcgg atgtgcactt
      661 gaagtggtgg ttgttcacgg tgccctccat gtacagcttc atgtgcatgt tctccttaat
      721 cagctcttcg cccttagaca ccatggtggc gtctagacca agtgacgatc acagcgatcc
      781 acaaacaaga accgcgaccc aaatcccggc tgcgacggaa ctagctgtgc cacacccggc
      841 gcgtccttat ataatcatcg gcgttcaccg cccca
//
