LOCUS       sequence-346119-       11195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CAG promoter expression plasmid for human codon optimized BE4max 
            C-to-T base editor with 
            SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R133
            3P/R1335Q/T1337R) and P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Walton RT, Christie KA, Whittaker MN, Kleinstiver BP
  TITLE     Unconstrained genome targeting with near-PAMless engineered 
            CRISPR-Cas9 variants.
  JOURNAL   Science. 2020 Apr 17;368(6488):290-296. doi: 
            10.1126/science.aba8853. Epub 2020 Mar 26.
  PUBMED    32217751
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aba8853"; journalName: "Science"; date: 
            "2020-04-17- 17"; volume: "368"; issue: "6488"; pages: "290-296"
COMMENT     Sequence Label: pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)
FEATURES             Location/Qualifiers
     source          1..11195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1809..1829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1830..2513
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             2610..6710
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6741..6989
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7020..7268
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7311..7331
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7350..7406
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7407..7409,7410..7412,7413..8126)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7407..7409,7410..7412,7413..8123)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7713..7732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8060..8081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8213..8232)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8278..8333
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8332..8351)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8694..8710)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8694..8710)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8707..8729)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8718..8734
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8742..8748,8749..8766,8767..8772))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8787..8808
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8867..9062
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8913..9048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9068..9202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9105..9124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9159..9178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9270..9287)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9441..10029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9521..9540)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10200..10991,10992..11060))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10823..10842
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11061..11165)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gsgcgcgccr ggsggggggg gggggggggg gggggggggs
      541 ggggsgrggs ggagaggkgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggrggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattctg cagtcgacgg
     1741 taccgcgggc ccgggatcca ccggtcgcca ccatgaaacg gacagccgac ggaagcgagt
     1801 tcgagtcacc aaagaagaag cggaaagtct cctcagagac tgggcctgtc gccgtcgatc
     1861 caaccctgcg ccgccggatt gaacctcacg agtttgaagt gttctttgac ccccgggagc
     1921 tgagaaagga gacatgcctg ctgtacgaga tcaactgggg aggcaggcac tccatctgga
     1981 ggcacacctc tcagaacaca aataagcacg tggaggtgaa cttcatcgag aagtttacca
     2041 cagagcggta cttctgcccc aataccagat gtagcatcac atggtttctg agctggtccc
     2101 cttgcggaga gtgtagcagg gccatcaccg agttcctgtc cagatatcca cacgtgacac
     2161 tgtttatcta catcgccagg ctgtatcacc acgcagaccc aaggaatagg cagggcctgc
     2221 gcgatctgat cagctccggc gtgaccatcc agatcatgac agagcaggag tccggctact
     2281 gctggcggaa cttcgtgaat tattctccta gcaacgaggc ccactggcct aggtacccac
     2341 acctgtgggt gcgcctgtac gtgctggagc tgtattgcat catcctgggc ctgccccctt
     2401 gtctgaatat cctgcggaga aagcagcccc agctgacctt ctttacaatc gccctgcagt
     2461 cttgtcacta tcagaggctg ccaccccaca tcctgtgggc cacaggcctg aagtctggag
     2521 gatctagcgg aggatcctct ggcagcgaga caccaggaac aagcgagtca gcaacaccag
     2581 agagcagtgg cggcagcagc ggcggcagcg acaagaagta cagcatcggc ctggccatcg
     2641 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
     2701 tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc ggagccctgc
     2761 tgttcgacag cggcgaaaca gccgagagaa cccggctgaa gagaaccgcc agaagaagat
     2821 acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac gagatggcca
     2881 aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa gaggataaga
     2941 agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac cacgagaagt
     3001 accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag gccgacctgc
     3061 ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc ctgatcgagg
     3121 gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg gtgcagacct
     3181 acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc aaggccatcc
     3241 tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag ctgcccggcg
     3301 agaagaagaa tggcctgttc ggaaacctga ttgccctgag cctgggcctg acccccaact
     3361 tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gctgagcaag gacacctacg
     3421 acgacgacct ggacaacctg ctggcccaga tcggcgacca gtacgccgac ctgtttctgg
     3481 ccgccaagaa cctgtccgac gccatcctgc tgagcgacat cctgagagtg aacaccgaga
     3541 tcaccaaggc ccccctgagc gcctctatga tcaagagata cgacgagcac caccaggacc
     3601 tgaccctgct gaaagctctc gtgcggcagc agctgcctga gaagtacaaa gagattttct
     3661 tcgaccagag caagaacggc tacgccggct acattgacgg cggagccagc caggaagagt
     3721 tctacaagtt catcaagccc atcctggaaa agatggacgg caccgaggaa ctgctcgtga
     3781 agctgaacag agaggacctg ctgcggaagc agcggacctt cgacaacggc agcatccccc
     3841 accagatcca cctgggagag ctgcacgcca ttctgcggcg gcaggaagat ttttacccat
     3901 tcctgaagga caaccgggaa aagatcgaga agatcctgac cttccgcatc ccctactacg
     3961 tgggccctct ggccagggga aacagcagat tcgcctggat gaccagaaag agcgaggaaa
     4021 ccatcacccc ctggaacttc gaggaagtgg tggacaaggg cgcttccgcc cagagcttca
     4081 tcgagcggat gaccaacttc gataagaacc tgcccaacga gaaggtgctg cccaagcaca
     4141 gcctgctgta cgagtacttc accgtgtata acgagctgac caaagtgaaa tacgtgaccg
     4201 agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc
     4261 tgttcaagac caaccggaaa gtgaccgtga agcagctgaa agaggactac ttcaagaaaa
     4321 tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga tcggttcaac gcctccctgg
     4381 gcacatacca cgatctgctg aaaattatca aggacaagga cttcctggac aatgaggaaa
     4441 acgaggacat tctggaagat atcgtgctga ccctgacact gtttgaggac agagagatga
     4501 tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga caaagtgatg aagcagctga
     4561 agcggcggag atacaccggc tggggcaggc tgagccggaa gctgatcaac ggcatccggg
     4621 acaagcagtc cggcaagaca atcctggatt tcctgaagtc cgacggcttc gccaacagaa
     4681 acttcatgca gctgatccac gacgacagcc tgacctttaa agaggacatc cagaaagccc
     4741 aggtgtccgg ccagggcgat agcctgcacg agcacattgc caatctggcc ggcagccccg
     4801 ccattaagaa gggcatcctg cagacagtga aggtggtgga cgagctcgtg aaagtgatgg
     4861 gccggcacaa gcccgagaac atcgtgatcg aaatggccag agagaaccag accacccaga
     4921 agggacagaa gaacagccgc gagagaatga agcggatcga agagggcatc aaagagctgg
     4981 gcagccagat cctgaaagaa caccccgtgg aaaacaccca gctgcagaac gagaagctgt
     5041 acctgtacta cctgcagaat gggcgggata tgtacgtgga ccaggaactg gacatcaacc
     5101 ggctgtccga ctacgatgtg gaccatatcg tgcctcagag ctttctgaag gacgactcca
     5161 tcgacaacaa ggtgctgacc agaagcgaca agaaccgggg caagagcgac aacgtgccct
     5221 ccgaagaggt cgtgaagaag atgaagaact actggcggca gctgctgaac gccaagctga
     5281 ttacccagag aaagttcgac aatctgacca aggccgagag aggcggcctg agcgaactgg
     5341 ataaggccgg cttcatcaag agacagctgg tggaaacccg gcagatcaca aagcacgtgg
     5401 cacagatcct ggactcccgg atgaacacta agtacgacga gaatgacaag ctgatccggg
     5461 aagtgaaagt gatcaccctg aagtccaagc tggtgtccga tttccggaag gatttccagt
     5521 tttacaaagt gcgcgagatc aacaactacc accacgccca cgacgcctac ctgaacgccg
     5581 tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg
     5641 actacaaggt gtacgacgtg cggaagatga tcgccaagag cgagcaggaa atcggcaagg
     5701 ctaccgccaa gtacttcttc tacagcaaca tcatgaactt tttcaagacc gagattaccc
     5761 tggccaacgg cgagatccgg aagcggcctc tgatcgagac aaacggcgaa accggggaga
     5821 tcgtgtggga taagggccgg gattttgcca ccgtgcggaa agtgctgagc atgccccaag
     5881 tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt cagcaaagag tctatcagac
     5941 ccaagaggaa cagcgataag ctgatcgcca gaaagaagga ctgggaccct aagaagtacg
     6001 gcggcttcct gtggcccacc gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg
     6061 gcaagtccaa gaaactgaag agtgtgaaag agctgctggg gatcaccatc atggaaagaa
     6121 gcagcttcga gaagaatccc atcgactttc tggaagccaa gggctacaaa gaagtgaaaa
     6181 aggacctgat catcaagctg cctaagtact ccctgttcga gctggaaaac ggccggaaga
     6241 gaatgctggc ctctgccaag cagctgcaga agggaaacga actggccctg ccctccaaat
     6301 atgtgaactt cctgtacctg gccagccact atgagaagct gaagggctcc cccgaggata
     6361 atgagcagaa acagctgttt gtggaacagc acaagcacta cctggacgag atcatcgagc
     6421 agatcagcga gttctccaag agagtgatcc tggccgacgc taatctggac aaagtgctgt
     6481 ccgcctacaa caagcaccgg gataagccca tcagagagca ggccgagaat atcatccacc
     6541 tgtttaccct gaccagactg ggagccccta gagccttcaa gtactttgac accaccatcg
     6601 accccaagca gtacagaagc accaaagagg tgctggacgc caccctgatc caccagagca
     6661 tcaccggcct gtacgagaca cggatcgacc tgtctcagct gggaggtgac agcggcggga
     6721 gcggcgggag cggggggagc actaatctga gcgacatcat tgagaaggag actgggaaac
     6781 agctggtcat tcaggagtcc atcctgatgc tgcctgagga ggtggaggaa gtgatcggca
     6841 acaagccaga gtctgacatc ctggtgcaca ccgcctacga cgagtccaca gatgagaatg
     6901 tgatgctgct gacctctgac gcccccgagt ataagccttg ggccctggtc atccaggatt
     6961 ctaacggcga gaataagatc aagatgctga gcggaggatc cggaggatct ggaggcagca
     7021 ccaacctgtc tgacatcatc gagaaggaga caggcaagca gctggtcatc caggagagca
     7081 tcctgatgct gcccgaagaa gtcgaagaag tgatcggaaa caagcctgag agcgatatcc
     7141 tggtccatac cgcctacgac gagagtaccg acgaaaatgt gatgctgctg acatccgacg
     7201 ccccagagta taagccctgg gctctggtca tccaggattc caacggagag aacaaaatca
     7261 aaatgctgtc tggcggctca aaaagaaccg ccgacggcag cgaattcgag cccaagaaga
     7321 agaggaaagt cggaggcggt ggaagcggcg caacaaactt ctctctgctg aaacaagccg
     7381 gagatgtcga agagaatcct ggaccgatgg tgagcaaggg cgaggagctg ttcaccgggg
     7441 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     7501 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     7561 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     7621 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     7681 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     7741 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     7801 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     7861 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     7921 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     7981 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     8041 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     8101 tcggcatgga cgagctgtac aagtgaagcg gccgcactcc tcaggtgcag gctgcctatc
     8161 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
     8221 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     8281 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     8341 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     8401 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     8461 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     8521 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     8581 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     8641 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
     8701 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     8761 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     8821 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
     8881 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8941 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     9001 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9061 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     9121 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     9181 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
     9241 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9301 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9361 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9421 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9481 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9541 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9601 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9661 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9721 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9781 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9841 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9901 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9961 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10021 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10081 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10141 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10201 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10261 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10321 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10381 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10441 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10501 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10561 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10621 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10681 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10741 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10801 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10861 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10921 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10981 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11041 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11101 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11161 ccgcgcacat ttccccgaaa agtgccacct gggtc
//
