LOCUS       sequence-34612-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DTX3L
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             88..105
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             555..572
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(625..643)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      639..686
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(810..829)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgcgaattcc ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcctg gttccgcgtg gtagtacttc ctatggtatt
      121 cagaaaggaa atcagccaga gggaagcatg gttttcactg tttcaagaga ctcacttcca
      181 ggttatgagt cctttggcac cattgtgatt acttattcta tgaaagcagg catacaaaca
      241 gaagaacacc caaacccagg aaagagatac cctggaatac agcgaactgc atacttgcct
      301 gataataagg aaggaaggaa ggttttgaaa ctgctttata gggcctttga ccaaaagctg
      361 atttttacag tggggtactc tcgcgtatta ggagtctcag atgtcatcac ttggaatgat
      421 attcaccaca aaacatcccg gtttggagga ccagaaatgt atggctatcc tgatccttct
      481 tacctgaaac gtgtcaaaga ggagctgaaa gccaaaggaa ttgagtgatg acgaagcttg
      541 cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      601 aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      661 ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg
      721 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
      781 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      841 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggntccgatt
      901 tagtgcttta cggcacctcg accccaaaaa ct
//
