LOCUS       sequence-34616-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BRPF3
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             545..562
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(615..633)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      629..676
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(800..819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctctgaaat aattttgttt actttaagaa ggagatatac catgcatcat catcatcatc
       61 acagcagcgg cagagaaaac ttgtatttcc agggcgacta caatggctca ggcagaagcc
      121 tcctgctgcc ctttgaagac cgcggagacc tggagccctt ggagctggtg tgggccaagt
      181 gccgaggcta cccctcctac cctgccttga tcatcgatcc caagatgccc cgggagggcc
      241 tcctgcacaa tggcgttccc atccctgtcc ccccgctgga cgtgctgaag ctgggagagc
      301 agaaacaggc agaggctgga gagaagctct tccttgtcct cttctttgac aacaagcgca
      361 cctggcagtg gcttccaagg gacaaagtcc tgcccttggg tgtggaagac accgtggaca
      421 agctcaagat gctggaaggc cgcaagacca gcatccgcaa gtcagtgcag gtggcctatg
      481 accgtgcgat gatccacctg agcagagtcc gggggtgatg acgaagcttg cggccgcact
      541 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      601 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      661 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcg
//
