LOCUS       sequence-346167-        4146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) 
            POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal 
            epitope tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Chen C, Masi R, Lintermann R, Wirthmueller L
  TITLE     Nuclear Import of Arabidopsis Poly(ADP-Ribose) Polymerase 2 Is 
            Mediated by Importin-alpha and a Nuclear Localization Sequence 
            Located Between the Predicted SAP Domains.
  JOURNAL   Front Plant Sci. 2018 Nov 1;9:1581. doi: 10.3389/fpls.2018.01581. 
            eCollection 2018.
  PUBMED    30455710
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2018-11-1"; volume: "9:1581. doi"; pages: " 
            10.3389/fpls.2018.01581. eCollection 2018"
COMMENT     Sequence Label: pENTR4 CDS A. thaliana PARP2
FEATURES             Location/Qualifiers
     source          1..4146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..48
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      293..379
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      471..498
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     506..529
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    548..647
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2565..2664)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2776..2795)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2787..3596
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2854..2873)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3686..4146,1..128)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
       61 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      121 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      181 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca
      241 tggatctcgg ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa
      301 acgaaaggct cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      361 tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg
      421 agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat
      481 cctgacggat ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct
      541 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      601 taagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatggcgaa
      661 caagctcaaa gtcgacgaac tccgtttaaa actcgccgag cgtggactca gtactactgg
      721 agtcaaagcc gttctggtgg agaggcttga agaggctatc gcagaagaca ctaagaagga
      781 agaatcaaag agcaagagga aaagaaattc ttctaatgat acttatgaat cgaacaaatt
      841 gattgcaatt ggcgaatttc gtgggatgat tgtgaaggaa ttgcgtgagg aagctattaa
      901 gagaggctta gatacaacag gaaccaaaaa ggatcttctt gagaggcttt gcaatgatgc
      961 taataacgtt tccaatgcac cagtcaaatc cagtaatggg acagatgaag ctgaagatga
     1021 caacaatggc tttgaagaag aaaagaaaga agagaaaatc gtaaccgcga caaagaaggg
     1081 tgcagcggtg ttagatcagt ggattcctga tgagataaag agtcagtacc atgttctaca
     1141 aaggggtgat gatgtttatg atgctatctt aaatcagaca aatgtcaggg ataataataa
     1201 caagttcttt gtcctacaag tcctagagtc ggatagtaaa aagacataca tggtttacac
     1261 cagatgggga agagttggtg tgaaaggaca aagtaagcta gatgggcctt atgactcatg
     1321 ggatcgtgcg atagagatat ttaccaataa gttcaatgac aagacaaaga attattggtc
     1381 tgacagaaag gagtttatcc cacatcccaa gtcctataca tggctcgaaa tggattacgg
     1441 aaaagaggaa aatgattcac cggtcaataa tgatattccg agttcatctt ccgaagttaa
     1501 acctgaacaa tcaaaactag atactcgggt tgccaagttc atctctctta tatgtaatgt
     1561 cagcatgatg gcacagcata tgatggaaat aggatataac gctaacaaat tgccactcgg
     1621 caagataagc aagtccacaa tttcaaaggg ttatgaagtg ctgaagagaa tatcggaggt
     1681 gattgatcgg tatgatagaa cgaggcttga ggaactgagt ggagagttct acacagtgat
     1741 acctcatgat tttggtttta agaaaatgag ccagtttgtt atagacactc ctcaaaagtt
     1801 gaaacagaaa attgaaatgg ttgaagcatt aggtgagatt gaactcgcaa caaagttgtt
     1861 gtccgtcgac ccgggattgc aggatgatcc tttatattat cactaccagc aacttaattg
     1921 tggtttgacg ccagtaggaa atgattcaga ggagttctct atggttgcta attacatgga
     1981 gaacactcat gcaaagacgc attcgggata tacggttgag attgctcaac tatttagagc
     2041 ttcgagagct gttgaagctg atcgattcca acagttttca agttcgaaga acaggatgct
     2101 actctggcac ggttcacgtc tcactaactg ggctggtatt ttatctcaag gtctgcgaat
     2161 agctcctcct gaagcgcctg taactggtta catgtttgga aaaggggttt actttgcgga
     2221 tatgttctcc aagagtgcga actattgcta tgccaacact ggcgctaatg atggcgttct
     2281 gctcctctgc gaggttgctt tgggagacat gaatgaactt ctgtattcag attataacgc
     2341 ggataatcta cccccgggaa agctaagcac aaaaggtgtg gggaaaacag caccaaaccc
     2401 atcagaggct caaacactag aagacggtgt tgttgttcca cttggcaaac cagtggaacg
     2461 ttcatgctcc aaggggatgt tgttgtacaa cgaatatata gtctacaatg tggaacaaat
     2521 caagatgcgt tatgtgatcc aagtcaaatt caactacaag cacgacccag ctttcttgta
     2581 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2641 cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc
     2701 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     2761 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     2821 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     2881 ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc
     2941 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
     3001 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctggtgatg
     3061 catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc
     3121 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
     3181 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
     3241 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
     3301 ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg
     3361 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
     3421 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
     3481 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
     3541 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
     3601 aattggttaa ttggttgtaa cattattcag attgggcccc gttccactga gcgtcagacc
     3661 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3721 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3781 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3841 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3901 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3961 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4021 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4081 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4141 tcggaa
//
