LOCUS       sequence-346169-        5110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Christian Frøkjær-Jensen, M Wayne Davis, Christopher E Hopkins, 
  TITLE     Single-copy insertion of transgenes in Caenorhabditis elegans
  PUBMED    18953339
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCFJ90 - Pmyo-2::mCherry::unc-54utr
FEATURES             Location/Qualifiers
     source          1..5110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(71..91)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(99..116)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(99..115)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(108..130)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     443..465
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(503..521)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        589..693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(694..762,763..1554)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(912..931)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1725..2313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2214..2233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2440..2462
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2459..2475
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2459..2475
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2495..2515
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3495..3519
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4387..4411)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
       61 taactgtttc caactttatt atacatagtt gataattcac tggccgtcgt tttacaacgt
      121 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      181 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      241 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
      301 caccgcatat ggtgcactct cagtgcaatc tgctctgatg ccgcatagtt aagccagccc
      361 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
      421 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
      481 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      541 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      601 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      661 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      721 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      781 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      841 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      901 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      961 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1021 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1081 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1141 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1201 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1261 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1321 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1381 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggtca
     1441 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1501 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1561 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1621 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1681 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1741 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     1801 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     1861 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     1921 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     1981 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2041 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2101 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2161 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2221 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2281 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2341 ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2401 gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca atttcacaca
     2461 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttgcattt
     2521 tatatctgag tagtatcctt tgctttaaat gtccataaaa ctaattttat aatcaataaa
     2581 acaacgtttg taaatcaact gagtttacaa gtagagacat tgagggatac tttcactatg
     2641 ctaaagtgat aatcgaccaa ataataactt cactttggta tttattcctg tcttataaat
     2701 gttatgtatg aattaaattc atatgcatat ggctcactct gacaattttt aataatcttc
     2761 cagatcaata ttgactaccg atgcgggtgg tcttttgctt tgaattctgc tgaactttac
     2821 accccgaaca gcaatgtgtg cttcagccta aaaaaaagta agtgtgttaa tcagtgcccc
     2881 cgattcttca ttttttgccc ctctctcccg tttcgtcggc aaaagaagag aaaataaaga
     2941 taagtctcaa gataggttgg taatcgctaa agtggttgtg tggataagag tagcaaaatg
     3001 gcaggaagag cactttgcgc gcacacactg tactcattgt tctggataaa attctctcgt
     3061 tgtttgccgt cggatgtctg cctctctgca ttgagccggc ttcttcacta tctttagtta
     3121 acctaaaatg ccgtttcttt tctcgtatcc ccactatccc gttgaggttc tctgctctct
     3181 tcgctcccta ccgccagcga gcaactatcc gtgggggcgc cttgctcgga agatgggggg
     3241 gaagaaagaa gatttttgct atttgcactt gagaaagaga cttttcctgc gtcgatggtc
     3301 agagaacagt gtgcagacac ttttcagcta cctagaatta caattggata tccccgcctc
     3361 ccaatccacc cacccaggga aaaagaaggg ctcgccgaaa atcaaagtta tctccaggct
     3421 cgcgcatccc accgagcggt tgacttctct ccaccacttt tcattttaac cctcgatcgt
     3481 cagacacaga aatgacaagt ttgtacaaaa aagcaggctt aatggtctca aagggtgaag
     3541 aagataacat ggcaattatt aaagagttta tgcgtttcaa ggtgcatatg gagggatctg
     3601 tcaatgggca tgagtttgaa attgaaggtg aaggagaagg ccgaccatat gagggaacac
     3661 aaaccgcaaa actaaaggta agtttaaaca tatatatact aactaaccct gattatttaa
     3721 attttcaggt aactaaaggc ggaccattac cattcgcctg ggacatcctc tctccacagt
     3781 tcatgtatgg aagtaaagct tatgttaaac atccggcaga tataccagat tatttgaaac
     3841 tttcattccc ggagggtttt aagtgggaac gcgtaatgaa ttttgaagac ggaggagttg
     3901 ttacagtgac gcaagactca aggtaagttt aaacagttcg gtactaacta accatacata
     3961 tttaaatttt cagcctccaa gatggagaat ttatttataa agtcaaactt cgaggaacga
     4021 atttcccctc ggatggacct gttatgcaga agaagactat gggatgggaa gcttcaagtg
     4081 aaagaatgta ccctgaagac ggtgctctta agggagagat taaacaacgt cttaaattga
     4141 aagatggagg acattacgat gctgaggtaa gtttaaacat gattttacta actaactaat
     4201 ctgatttaaa ttttcaggtg aagacaactt acaaagccaa aaaaccagtt cagctgccag
     4261 gagcgtacaa tgttaatatt aaactggata tcacctccca caacgaggat tacactatcg
     4321 ttgagcaata tgaaagagct gaagggcggc actcgacagg tggcatggat gaattgtata
     4381 agtagtaccc agctttcttg tacaaagtgg gtgatatctg agctccgcat cggccgctgt
     4441 catcagatcg ccatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     4501 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
     4561 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     4621 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     4681 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     4741 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     4801 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     4861 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     4921 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt
     4981 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     5041 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     5101 tttgaaagtg
//
