LOCUS       sequence-34617-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: OTUD5
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..67
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             80..100
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             631..648
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(701..719)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      715..762
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(886..905)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgaattcct ctgaatattt tgtttacttt aagaaggaga tataccatgc atcatcatca
       61 tcatcacagc agcggcagag aaaacttgta tttccagggc gcaggctaca acagtgagga
      121 cgagtatgag gcggctgcag cacgcatcga ggctatggac cctgccactg tcgagcagca
      181 ggagcattgg tttgaaaagg ccctacgaga caagaagggc ttcatcatca agcagatgaa
      241 ggaggatggc gcctgtctct tccgggctgt agctgaccag gtgtatggag accaggacat
      301 gcatgaggtt gtgcgaaagc attgcatgga ctatctgatg aagaatgccg actacttctc
      361 caactatgtc acagaggact ttaccaccta cattaacagg aagcggaaaa acaattgcca
      421 tggcaaccac attgagatgc aggccatggc agagatgtac aaccgtcctg tggaggtgta
      481 ccagtacagc acagaaccca tcaacacatt ccatgggata catcaaaacg aggacgaacc
      541 cattcgtgtt agctaccatc ggaatatcca ctataattca gtggtgaatc ctaacaaggc
      601 ctgatgacga agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
      661 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
      721 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      781 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      841 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      901 tcccttcctt ctcgccacgt tcgccggctt ttcccgtcaa gctctaaatc gggggctccc
      961 tt
//
