LOCUS       sequence-346184-       10249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 6xHis-MBP tagged CMtCas12a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Nguyen LT,  Macaluso NC,  Jain PK
  TITLE     A Combinatorial Approach towards Adaptability of 22 Functional 
            Cas12a Orthologs for Nucleic Acid Detection in Clinical Samples
  JOURNAL   medRxiv 2021.07.21
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            2021.07.21"
COMMENT     Sequence Label: 6xHis-MBP-huCMtCas12a
FEATURES             Location/Qualifiers
     source          1..10249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     106..125
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     209..228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        209..227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    228..252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             267..289
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             308..325
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             335..1435
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1409..1432
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1448..1468
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5186..5233
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5240..5320
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             5321..5338
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5391..5409)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5405..5452
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5489..5944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5576..5595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5786..5807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6037..6852)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6037..6852)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6760..6779
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6844..6863
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6974..7562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7463..7482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7716..7733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7748..7887
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7813..7832)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7973..7995
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7989..8180)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8989..10071)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10033..10052
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10072..10149)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat ataccatggg
      301 ttcttctcac catcaccatc accatggttc ttctatgaaa atcgaagaag gtaaactggt
      361 aatctggatt aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga
      421 gaaagatacc ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc
      481 acaggttgcg gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg
      541 tggctacgct caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa
      601 gctgtatccg tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat
      661 cgctgttgaa gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac
      721 ctgggaagag atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat
      781 gttcaacctg caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc
      841 gttcaagtat gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc
      901 gaaagcgggt ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac
      961 cgattactcc atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg
     1021 cccgtgggca tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc
     1081 gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc
     1141 cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga
     1201 aggtctggaa gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga
     1261 ggaagagttg gcgaaagatc cacgtattgc cgccactatg gaaaacgccc agaaaggtga
     1321 aatcatgccg aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat
     1381 caacgccgcc agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactaatgg
     1441 gatcgaggaa aacctgtact tccaatccaa tatgaacaat tacgacgagt tcaccaagct
     1501 gtatcctatc cagaaaacca tccggtttga gctgaagcca cagggcagaa ccatggagca
     1561 cctggagaca ttcaacttct ttgaggagga ccgggataga gccgagaagt ataagatcct
     1621 gaaggaggcc atcgacgagt accacaagaa gtttatcgat gagcacctga ccaatatgtc
     1681 cctggattgg aactctctga agcagatcag cgagaagtac tataagagca gggaggagaa
     1741 ggacaagaag gtgttcctgt ccgagcagaa gaggatgcgc caggagatcg tgtctgagtt
     1801 taagaaggac gatcgcttca aggacctgtt ttccaagaag ctgttctctg agctgctgaa
     1861 ggaggagatc tacaagaagg gcaaccacca ggagatcgac gccctgaaga gcttcgataa
     1921 gttttccggc tatttcatcg gcctgcacga gaataggaag aacatgtact ccgacggcga
     1981 tgagatcacc gccatctcca atcgcatcgt gaatgagaac ttccccaagt ttctggataa
     2041 cctgcagaag taccaggagg ccaggaagaa gtatcctgag tggatcatca aggccgagag
     2101 cgccctggtg gcccacaata tcaagatgga cgaggtgttc tccctggagt actttaataa
     2161 ggtgctgaac caggagggca tccagcggta caacctggcc ctgggcggct atgtgaccaa
     2221 gagcggcgag aagatgatgg gcctgaatga tgccctgaac ctggcccacc agtccgagaa
     2281 gagctccaag ggcagaatcc acatgacccc cctgttcaag cagatcctgt ccgagaagga
     2341 gtccttctct tacatccccg acgtgtttac agaggattct cagctgctgc ctagcatcgg
     2401 cggcttcttt gcccagatcg agaatgacaa ggatggcaac atcttcgacc gggccctgga
     2461 gctgatctct agctacgccg agtatgatac cgagcggatc tatatcagac aggccgacat
     2521 caatagagtg tccaacgtga tctttggaga gtggggcacc ctgggaggcc tgatgaggga
     2581 gtacaaggcc gactctatca atgatatcaa cctggagcgc acatgcaaga aggtggacaa
     2641 gtggctggat tctaaggagt ttgccctgag cgatgtgctg gaggccatca agaggaccgg
     2701 caacaatgac gccttcaacg agtatatctc caagatgcgg acagccagag agaagatcga
     2761 tgccgcccgc aaggagatga agttcatcag cgagaagatc tccggcgatg aggagtctat
     2821 ccacatcatc aagaccctgc tggacagcgt gcagcagttc ctgcacttct ttaatctgtt
     2881 taaggcaagg caggacatcc cactggatgg agccttctac gccgagtttg acgaggtgca
     2941 cagcaagctg tttgccatcg tgcccctgta taacaaggtg cggaactatc tgaccaagaa
     3001 caatctgaac acaaagaaga tcaagctgaa tttcaagaac cctacactgg ccaatggctg
     3061 ggaccagaac aaggtgtacg attatgcctc cctgatcttt ctgcgggacg gcaattacta
     3121 tctgggcatc atcaatccta agagaaagaa gaacatcaag ttcgagcagg gctctggcaa
     3181 cggccccttc taccggaaga tggtgtataa gcagatcccc ggccctaata agaacctgcc
     3241 aagagtgttc ctgacctcca caaagggcaa gaaggagtat aagccctcta aggagatcat
     3301 cgagggctac gaggccgaca agcacatcag gggcgataag ttcgacctgg atttttgtca
     3361 caagctgatc gatttcttta aggagtccat cgagaagcac aaggactggt ctaagttcaa
     3421 cttctacttc agcccaaccg agagctatgg cgacatctct gagttctacc tggatgtgga
     3481 gaagcagggc tatcgcatgc actttgagaa tatcagcgcc gagacaatcg acgagtatgt
     3541 ggagaagggc gatctgtttc tgttccagat ctacaacaag gattttgtga aggccgccac
     3601 cggcaagaag gacatgcaca caatctactg gaatgccgcc ttcagccccg agaacctgca
     3661 ggacgtggtg gtgaagctga acggcgaggc cgagctgttt tatagggaca agtccgatat
     3721 caaggagatc gtgcaccgcg agggcgagat cctggtgaat aggacctaca acggccgcac
     3781 accagtgccc gacaagatcc acaagaagct gaccgattat cacaatggcc ggacaaagga
     3841 cctgggcgag gccaaggagt acctggataa ggtgagatac ttcaaggccc actatgacat
     3901 caccaaggat cggagatacc tgaacgacaa gatctatttc cacgtgcctc tgaccctgaa
     3961 cttcaaggcc aacggcaaga agaatctgaa caagatggtc atcgagaagt tcctgtccga
     4021 tgagaaggcc cacatcatcg gcatcgacag gggcgagcgc aatctgctgt actattccat
     4081 catcgacagg tctggcaaga tcatcgatca gcagagcctg aatgtgatcg acggctttga
     4141 ttatcgggag aagctgaacc agagagagat cgagatgaag gatgcccgcc agtcttggaa
     4201 cgccatcggc aagatcaagg acctgaagga gggctacctg agcaaggccg tgcacgagat
     4261 caccaagatg gccatccagt ataatgccat cgtggtcatg gaggagctga actacggctt
     4321 caagcggggc cggttcaagg tggagaagca gatctatcag aagttcgaga atatgctgat
     4381 cgataagatg aactacctgg tgtttaagga cgcacctgat gagtccccag gaggcgtgct
     4441 gaatgcctac cagctgacaa acccactgga gtctttcgcc aagctgggca agcagaccgg
     4501 catcctgttt tacgtgccag ccgcctatac atccaagatc gaccccacca caggcttcgt
     4561 gaatctgttt aacacctcct ctaagacaaa cgcccaggag cggaaggagt tcctgcagaa
     4621 gtttgagagc atctcctatt ctgccaagga tggcggcatc tttgccttcg cctttgacta
     4681 cagaaagttc ggcaccagca agacagatca caagaacgtg tggaccgcct atacaaacgg
     4741 cgagaggatg cgctacatca aggagaagaa gcggaatgag ctgtttgacc cttctaagga
     4801 gatcaaggag gccctgacca gctccggcat caagtacgat ggcggccaga acatcctgcc
     4861 agacatcctg aggagcaaca ataacggcct gatctacaca atgtattcta gcttcatcgc
     4921 cgccatccag atgcgcgtgt acgacggcaa ggaggattat atcatcagcc ccatcaagaa
     4981 ctccaagggc gagttcttta ggaccgaccc caagaggcgc gagctgccta tcgacgccga
     5041 tgccaatggc gcctacaaca tcgccctgag gggagagctg acaatgaggg caatcgcaga
     5101 gaagttcgac cctgatagcg agaagatggc caagctggag ctgaagcaca aggattggtt
     5161 cgagtttatg cagaccagag gcgacaaaag gccggcggcc acgaaaaagg ccggccaggc
     5221 aaaaaagaaa aagggatcct acccatacga tgttccagat tacgcttatc cctacgacgt
     5281 gcctgattat gcatacccat atgatgtccc cgactatgcg caccaccacc accaccactg
     5341 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     5401 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     5461 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     5521 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5581 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5641 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5701 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5761 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5821 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5881 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaactagt aacgtttaca
     5941 atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6001 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     6061 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     6121 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     6181 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     6241 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     6301 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     6361 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     6421 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     6481 cagcgcatca acaatgtttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     6541 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     6601 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     6661 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     6721 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     6781 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     6841 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     6901 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7021 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7081 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     7141 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7201 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7261 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7321 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7381 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7441 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7501 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7561 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7621 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7681 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7741 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg
     7801 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     7861 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     7921 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7981 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     8041 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     8101 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     8161 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta
     8221 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     8281 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     8341 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt
     8401 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc
     8461 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     8521 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     8581 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     8641 acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg
     8701 ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg
     8761 ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc
     8821 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc
     8881 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga
     8941 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
     9001 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9061 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
     9121 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
     9181 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
     9241 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt
     9301 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
     9361 gataccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
     9421 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
     9481 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
     9541 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
     9601 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
     9661 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
     9721 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
     9781 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
     9841 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
     9901 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     9961 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
    10021 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
    10081 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
    10141 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
    10201 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatg
//
