LOCUS       sequence-34619-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SIRT6
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aatattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggttc cgcgtggtag tatgtcggtg aattacgcgg cggggctgtc
      121 gccgtacgcg gacaagggca agtgcggcct cccggagatc ttcgaccccc cggaggagct
      181 ggagcggaag gtgtgggaac tggcgaggct ggtctggcag tcttccagtg tggtgttcca
      241 cacgggtgcc ggcatcagca ctgcctctgg catccccgac ttcaggggtc cccacggagt
      301 ctggaccatg gaggagcgag gtctggcccc caagttcgac accacctttg agagcgcgcg
      361 gcccacgcag acccacatgg cgctggtgca gctggagcgc gtgggcctcc tccgcttcct
      421 ggtcagccag aacgtggacg ggctccatgt gcgctcaggc ttccccaggg acaaactggc
      481 agagctccac gggaacatgt ttgtggaaga atgtgccaag tgtaagacgc agtacgtccg
      541 agacacagtc gtgggcacca tgggcctgaa ggccacgggc cggctctgca ccgtggctaa
      601 ggcaaggggg ctgcgagcct gcaggggaga gctgagggac accatcctag actgggagga
      661 ctccctgccc gaccgggacc tggcactcgc cgatga
//
