LOCUS       sequence-34620-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PP-CDPK2 (3PM8)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             583..600
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             650..667
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(720..738)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      734..781
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttccctctga atattttgtt taccttaaga aggagatata ctatgcacgt ggaactgtca
       61 tcgaccctgc tgaaaaacct gaaaaacttc aaaaaagaaa acgaactgaa gaaaattgcg
      121 ctgaccatta tcgccaaaca cctgtgcgat gtggaaatca acaacctgcg taacatcttc
      181 atcgcactgg atgttgacaa tagcggtacc ctgagctctc aagaaatcct ggacggcctg
      241 aagaaaattg gttatcagaa aatcccgccg gatattcatc aagttctgcg cgatattgac
      301 agcaatgctt ctggccagat tcactatacc gactttctgg cagctaccat cgataaacaa
      361 acgtacctga aaaaagaagt ctgtctgatt ccgttcaaat ttttcgatat tgacggcaac
      421 ggtaaaatta gtgtggaaga actgaaacgt atctttggcc gcgatgacat tgaaaatccg
      481 ctgatcgaca aagcgattga ttccctgctg caggaagtgg atctgaacgg tgatggtgaa
      541 atcgacttcc acgaatttat gctgatgatg tcaaaaaaaa aacaccatca tcaccaccat
      601 tgaggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca
      661 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      721 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      781 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      841 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cactt
//
