LOCUS       sequence-346200-        8111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRIM46 tagged with mCherry .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   van Beuningen SFB, Will L, Harterink M, Chazeau A, van Battum EY, 
            Frias CP, Franker MAM, Katrukha EA, Stucchi R, Vocking K, Antunes 
            AT, Slenders L, Doulkeridou S, Sillevis Smitt P, Altelaar AFM, Post 
            JA, Akhmanova A, Pasterkamp RJ, Kapitein LC, de Graaff E, Hoogenraad
            CC
  TITLE     TRIM46 Controls Neuronal Polarity and Axon Specification by Driving 
            the Formation of Parallel Microtubule Arrays.
  JOURNAL   Neuron. 2015 Dec 16;88(6):1208-1226. doi: 
            10.1016/j.neuron.2015.11.012. Epub 2015 Dec 6.
  PUBMED    26671463
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2015.11.012"; journalName: "Neuron"; date: 
            "2015-12-16- 16"; volume: "88"; issue: "6"; pages: "1208-1226"
COMMENT     Sequence Label:  mCherry-Trim46
FEATURES             Location/Qualifiers
     source          1..8111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2509..2643
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2546..2565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2600..2619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2670..2686)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2670..2686)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2683..2705)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2694..2710
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2718..2724,2725..2742,2743..2748))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(2900..2917)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3071..3659)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3151..3170)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3830..4621,4622..4690))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4453..4472
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     4878..4896
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4934..4956)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5056..5075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5307..5329
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5321..5338
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5322..5338
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        5904..6283
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6284..6487
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6438..6458
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6484..6508
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          6623..7449
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      7474..7483
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7480..8111,1..76)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYGAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7625..7643)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7893..7912
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
       61 ggacgagctg tacaagggcg cgcctgaatt catggctgag ggtgaggata tgcagacctt
      121 cacttccatc atggatgcac tagtccgcat cagtacaagc atgaagaaca tggagaagga
      181 actgctgtgc ccagtgtgcc aagagatgta caagcagcca ctagtgctgc cttgtaccca
      241 caacgtgtgc caggcctgtg cccgggaggt tttgggccag caggggtaca taggccatgg
      301 tggggatccg agttcggagc ccacttctcc tgcttccacc ccttctaccc gcagcccccg
      361 cctctctcgg agaactctcc ccaagccaga tcgcttggac cggctcctta agtcaggctt
      421 tgggacatac cctgggcgga agcgtggtgc cttgcacccc cagacgatcc tattcccgtg
      481 cccagcctgc caaggcgatg tggagctggg ggagaggggc ctttccgggc ttttccgaaa
      541 cctgacccta gagcgagtgg tggagaggta ccgccagagt gtgagtgtgg gaggagccat
      601 cctgtgccag ttgtgcaagc ccccaccact agaggccacc aagggctgca cagagtgtcg
      661 agctaccttc tgtaacgaat gcttcaagct cttccacccc tggggcactc agaaggcgca
      721 gcatgagccc acgctaccca ctctctcatt ccgccccaag ggtctgatgt gtccagacca
      781 caaggaagag gtgacccact actgtaagac atgtcaacga ctggtgtgcc aactctgcag
      841 agtacggcgt acccacagtg ggcacaagat cacaccagtg ctcagtgcct accaggccct
      901 caaggataag ctaacaaaga gcctggcata catcttggga aatcaggaca ctgtgcagac
      961 ccagatttgt gagctggaag agaccatcag gcacactgag gtgagtggtc agcaagcaaa
     1021 agaggaggtg tcccagctgg ttcggggact aggggctgtg ctggaagaga agcgggcctc
     1081 actgcttcag gccattgaag aatgccagca agagcgctta tcccggctca gcgcccagat
     1141 ccatgaacac cagagcctgc tggatggctc gggtctggtg ggttatgcgc aggaagtcct
     1201 taaggaaaca gaccagcctt gttttgtaca agcagccaaa cagctgcata acaggattgc
     1261 ccgagccact gaggccctcc agacattccg gccagctgcc agctcctcct tccgccactg
     1321 ccagctggat gtggggcgtg agatgaagct gttgaccgag cttagcttcc tgagagtgcc
     1381 cgaggcgccc gtcatagaca cccaacgcac ctttgcctat gatcaaatct tcctgtgctg
     1441 gcggctgccc cctcactcac cacctgcctg gcattacacc gttgagttcc ggcgcacaga
     1501 tgtccccgcc cagccaggcc ctacacgctg gcagcgccgg gaagaggtga ggggcaccag
     1561 cgcccttctt gagaaccccg acacgggctc tgtgtacgtg ctgcgtgtcc gtggctgcaa
     1621 caaggctggc tacggagagt acagtgaaga tgtgcacctg cacacacccc cagcccctgt
     1681 cctgcacttc ttccttgatg gccgctgggg tgcgagccga gagcggctag ccatcagcaa
     1741 ggaccagcga gcggtgcgga gtatcccagg gcttcccttg ctgctggctg ctgagcggct
     1801 gctcacgggc tgccacctga gtgtggacgt ggtcttgggt gatgtggctg tgacccaggg
     1861 ccgcagctac tgggcctgtg cggtagaccc tgcctcctac ttggtcaagg tgggcgtcgg
     1921 gctggagagc aaacttcagg aaagcttcca gggtgccccg gatgtgatca gccccagata
     1981 cgacccggac agtgggcacg acagcggtgc cgaggacgct gccgtggagg cgttaccacc
     2041 ctttgctttc ctgaccatcg gcatgggcaa gatcctgctg ggttccggag cgagttccaa
     2101 tgcggggctg acagggaggg atggccccac caccagctgc acagtgcccc tgccaccccg
     2161 cctaggtatc tgtttggact acgagcgggg gcgggtctcc ttcctggatg ccgtgtcctt
     2221 ccgtgggctg ctcgagtgcc ccctggactg ctcagggcct gtgtgccctg ccttctgctt
     2281 cattgggggt ggcgctgttc agctgcagga gcccgtgggc accaagcctg agaggaaagt
     2341 caccattggg ggctttgcca aactggactg agtcgactgc ggccgcctct agataattca
     2401 ttgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     2461 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2521 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2581 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     2641 atcgatccgt accgagctcg cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat
     2701 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2761 taatgagtga gctaactaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2821 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2881 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2941 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3001 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3061 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3121 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3181 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3241 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3301 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3361 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3421 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3481 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3541 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3601 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3661 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3721 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3781 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3841 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3901 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3961 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4021 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4081 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4141 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4201 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4261 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4321 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4381 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4441 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4501 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4561 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4621 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4681 gaatactcat actcttcctt tttcaataag cggcctcgag ttattattga agcatttatc
     4741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4801 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4861 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     4921 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4981 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5041 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgaagacgt
     5101 cgcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
     5161 taaaaaaaat tagtcagggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5221 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     5281 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     5341 ccgtgtcaag gacggtgact gcagtgaata ataaaatgtg tgtttgtccg aaatacgcgt
     5401 tttgagattt ctgtcgccga ctaaattcat gtcgcgcgat agtggtgttt atcgccgata
     5461 gagatggcga tattggaaaa atcgatattt gaaaatatgg catattgaaa atgtcgccga
     5521 tgtgagtttc tgtgtaactg atatcgccat ttttccaaaa gtgatttttg ggcatacgcg
     5581 atatctggcg atagcgctta tatcgtttac gggggatggc gatagacgac tttggtgact
     5641 tgggcgattc tgtgtgtcgc aaatatcgca gtttcgatat aggtgacaga cgatatgagg
     5701 ctatatcgcc gatagaggcg acatcaagct ggcacatggc caatgcatat cgatctatac
     5761 attgaatcaa tattggccat tagccatatt attcattggt tatatagcat aaatcaatat
     5821 tggctattgg ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc
     5881 atgtccaaca ttaccgccat gttgacattg attattgact agttattaat agtaatcaat
     5941 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     6001 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     6061 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     6121 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     6181 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     6241 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     6301 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     6361 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     6421 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     6481 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     6541 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg
     6601 gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt
     6661 ggcttcttat gcatgctata ctgtttttgg cttggggtct atacaccccc gcttcctcat
     6721 gttataggtg atggtatagc ttagcctata ggtgtgggtt attgaccatt attgaccact
     6781 cccctattgg tgacgatact ttccattact aatccataac atggctcttt gccacaactc
     6841 tctttattgg ctatatgcca atacactgtc cttcagagac tgacacggac tctgtatttt
     6901 tacaggatgg ggtctcattt attatttaca aattcacata tacaacacca ccgtccccag
     6961 tgcccgcagt ttttattaaa cataacgtgg gatctccacg cgaatctcgg gtacgtgttc
     7021 cggacatggg ctcttctccg gtagcggcgg agcttctaca tccgagccct gctcccatgc
     7081 ctccagcgac tcatggtcgc tcggcagctc cttgctccta acagtggagg ccagacttag
     7141 gcacagcacg atgcccacca ccaccagtgt gccgcacaag gccgtggcgg tagggtatgt
     7201 gtctgaaaat gagctcgggg agcgggcttg caccgctgac gcatttggaa gacttaaggc
     7261 agcggcagaa gaagatgcag gcagctgagt tgttgtgttc tgataagagt cagaggtaac
     7321 tcccgttgcg gtgctgttaa cggtggaggg cagtgtagtc tgagcagtac tcgttgctgc
     7381 cgcgcgcgcc accagacata atagctgaca gactaacaga ctgttccttt ccatgggtct
     7441 tttctgcagt caccgtcctt gacacgaagc ttcgccacca tggtgagcaa gggcgaggag
     7501 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     7561 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     7621 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     7681 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     7741 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     7801 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     7861 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     7921 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     7981 cagaggctga agctgaagga cggcggccac tacggcgctg aggtcaagac cacctacaag
     8041 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     8101 tcccacaacg a
//
