LOCUS       sequence-346210-       10414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterodimerization with iLID, Rac1 and RhoG activation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10414)
  TITLE     Gadella lab plasmids
REFERENCE   2  (bases 1 to 10414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLV-SspB-HaloTag-TRIO-DHPH1
FEATURES             Location/Qualifiers
     source          1..10414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    630..654
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             694..1023
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     regulatory      1039..1048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3337..3361)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3414..3455
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3479..4067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3532..3552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3950..3961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4082..4398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4249..4384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4446..4493
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4512..4910
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4997..5230
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5302..5423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5339..5358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5393..5412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5463..5598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5618..5637)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5619..5637)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5647..5664)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5647..5663)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5656..5678)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5805..6260
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5892..5911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6102..6123
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6286..6390
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6391..6459,6460..7251)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6609..6628)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7422..8010
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7911..7930
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8164..8181
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8298..8319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8334..8339,8340..8357,8358..8364)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8372..8388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8377..8399
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8396..8412
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8396..8412
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8431..8451
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8433..8451
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8479..8705
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8706..8886
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8933..9058
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9551..9784
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9969..10013
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10280..10397
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag gatccactag
      601 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctttaaag
      661 gaaccaattc agtcgactgg atccggtacc atgagctccc cgaaacgccc taagctgctg
      721 cgtgaatatt acgattggct ggttgataac agctttaccc catatctggt ggtggatgcc
      781 acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag acggtcagat cgtgctgaat
      841 ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg attttatcca gttcaacgcc
      901 cgctttaagg gcgtgtctcg tgaactgtat atcccgatgg gtgccgctct ggccatttac
      961 gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag aaatctatga cgagctgaat
     1021 attggtagtg gaccggtcgc caccatggcc gagatcggca ccggcttccc cttcgacccc
     1081 cactacgtgg aggtgctggg cgagcgcatg cactacgttg acgtgggccc ccgcgacggc
     1141 acccccgtgc tgttcctgca cggcaacccc accagcagct acgtgtggcg caacatcatc
     1201 ccccacgtgg cccccaccca ccgcactatc gcccccgacc tgatcggcat gggcaagagc
     1261 gacaagcccg acctgggcta cttcttcgac gaccacgtgc gcttcatgga cgccttcatc
     1321 gaggccctgg gcctggagga ggtggtgctg gtgatccacg actggggcag cgccctgggc
     1381 ttccactggg ccaagcgcaa ccccgagcgc gtgaagggca tcgccttcat ggagttcatc
     1441 cgccccatcc ccacctggga cgagtggccc gagttcgccc gcgagacctt ccaggccttc
     1501 cgcaccaccg acgtgggccg caagctgatc atcgaccaga acgtgttcat cgagggcacc
     1561 ctgcccatgg gcgtggtgcg ccccctgacc gaggtggaga tggaccacta ccgcgagccc
     1621 ttcctgaacc ccgtggaccg cgagcccctg tggcgcttcc ccaacgagct gcccatcgcc
     1681 ggcgagcccg ccaacatcgt ggccctggtg gaggagtaca tggactggct gcaccagagc
     1741 cccgtgccca agctgctgtt ctggggcacc cccggcgtgc tgatcccccc cgccgaggcc
     1801 gcccgcctgg ccaagagcct gcccaacgtt aaggccgtgg acatcggccc cggcctgaac
     1861 ctgctccagg aggacaaccc cgacctgatc ggcagcgaga tcgcccgctg gctgagcacc
     1921 ctggagatca gcggcgtgta caagtccgga ctcagatctc gagctaggaa agagttcata
     1981 atggctgagc tcattcaaac tgaaaaggct tatgtaagag acctccggga atgtatggat
     2041 acgtacctgt gggaaatgac cagtggcgtg gaagagattc cacctggcat tgtaaacaaa
     2101 gaactcatca tcttcggaaa catgcaagaa atctacgaat ttcataataa catattccta
     2161 aaggagctgg aaaaatatga acagttgcca gaggatgttg gacattgttt tgttacttgg
     2221 gcagacaagt ttcagatgta tgtcacatat tgcaaaaata agcctgattc tactcagctg
     2281 atattggaac atgcagggtc ctattttgac gagatacagc agcgacatgg attagccaat
     2341 tccatttctt cctaccttat taaaccagtt cagcgaataa cgaagtatca gctcctttta
     2401 aaagagctgc tgacgtgctg tgaggaagga aagggagaga ttaaagatgg cctggaggtg
     2461 atgctcagcg tgccgaagcg agccaatgac gccatgcacc tcagcatgct ggaagggttt
     2521 gatgaaaaca ttgagtctca gggagaactc atcctacagg aatccttcca agtgtgggac
     2581 ccaaaaacct taattcgaaa gggtcgagaa cggcatctct tcctttttga aatgtcctta
     2641 gtatttagta aagaagtgaa agattccagt gggagaagca agtaccttta taaaagcaaa
     2701 ttgtttacct cagagttggg tgtcacagaa catgttgaag gagacccttg caaatttgca
     2761 ctgtgggtgg ggagaacacc aacttcagat aataaaattg tccttaaggc ttccagcata
     2821 gagaacaagc aggactggat aaagcatatc cgcgaagtca tccaggagcg gacgatccac
     2881 ctgaagggag ccctgaagga gcccattcac atccctaaga ccgctcccgc cacaagacag
     2941 aagggaagga gggatggaga ggatctggac agccaaggag acggcagcag ccagcctgat
     3001 acgatttcca tcgcctcacg gacgtctcag aacacgctgg acagcgataa gctctctggt
     3061 ggctgtgagc tgacagtggt gatccatgac ttcaccgctt gcaacagcaa cgagctgacc
     3121 atccgacggg gccagaccgt ggaagttctg gagcggccgc atgacaagcc tgactggtgt
     3181 ctggtgcgga ccactgaccg ctccccagcg gcagaaggcc tggtcccctg tggttcactg
     3241 tgcatcgccc actccagaag tagcatggaa atggagggca tcttcaacca caaagactcg
     3301 ctctccgtct ccagcaatga cgcctaggat atctagaccc agctttcttg tacaaagtgg
     3361 ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc
     3421 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa tgatcgacaa
     3481 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3541 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3601 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3661 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3721 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3781 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3841 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     3901 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3961 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4021 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gcgatggtac
     4081 cggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4141 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4201 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     4261 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     4321 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     4381 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     4441 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     4501 ggaactaaac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     4561 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     4621 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     4681 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     4741 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     4801 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     4861 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcacaattcg
     4921 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     4981 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     5041 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     5101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     5161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     5221 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     5281 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     5341 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5401 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     5461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5521 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5581 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     5641 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     5701 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     5761 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     5821 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5881 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5941 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6001 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     6061 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     6121 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     6181 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     6241 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6301 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6361 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6421 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6481 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6541 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6601 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6661 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6721 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6781 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6841 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6901 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6961 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7021 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7081 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7141 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7201 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7261 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7321 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7381 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7441 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7501 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7561 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7621 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7681 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7741 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7801 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7861 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7921 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7981 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8041 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8101 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8161 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8221 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     8281 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8341 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8401 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     8461 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     8521 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     8581 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     8641 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     8701 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     8761 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     8821 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     8881 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     8941 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     9001 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     9061 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     9121 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     9181 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     9241 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     9301 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     9361 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     9421 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     9481 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     9541 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     9601 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     9661 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     9721 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     9781 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     9841 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     9901 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     9961 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
    10021 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
    10081 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
    10141 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
    10201 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
    10261 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
    10321 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    10381 caaaaattca aaattttatc gataagcttg ggag
//
