LOCUS       sequence-346212-       10173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterodimerization with iLID, Rac1 activation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Mahlandt EK, Palacios Martinez S, Arts JJG, Tol S, van Buul JD, 
            Goedhart J
  TITLE     Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control
            Rho GTPase activity on a global to subcellular scale, enabling 
            precise control over vascular endothelial barrier strength.
  JOURNAL   Elife. 2023 Jul 14;12:RP84364. doi: 10.7554/eLife.84364.
  PUBMED    37449837
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-07-14"; volume: "12:RP84364. doi"; pages: " 
            10.7554/eLife.84364"
COMMENT     Sequence Label: pLV-SspB-HaloTag-TIAM1-DHPH
FEATURES             Location/Qualifiers
     source          1..10173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    630..654
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             694..1023
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     regulatory      1039..1048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3096..3120)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3173..3214
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3238..3826
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3291..3311)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3709..3720)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3841..4157
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4008..4143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4205..4252
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4271..4669
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4756..4989
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5061..5182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5098..5117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5152..5171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5222..5357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5377..5396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5378..5396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5406..5423)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5406..5422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5415..5437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5564..6019
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5651..5670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5861..5882
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6045..6149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6150..6218,6219..7010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6368..6387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7181..7769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7670..7689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7923..7940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8057..8078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8093..8098,8099..8116,8117..8123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8131..8147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8136..8158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8155..8171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8155..8171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8190..8210
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8192..8210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8238..8464
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8465..8645
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8692..8817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9310..9543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9728..9772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10039..10156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag gatccactag
      601 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctttaaag
      661 gaaccaattc agtcgactgg atccggtacc atgagctccc cgaaacgccc taagctgctg
      721 cgtgaatatt acgattggct ggttgataac agctttaccc catatctggt ggtggatgcc
      781 acatacctgg gcgtgaacgt gcccgtggag tatgtgaaag acggtcagat cgtgctgaat
      841 ctgtctgcaa gtgcgaccgg caacctgcaa ctgacaaatg attttatcca gttcaacgcc
      901 cgctttaagg gcgtgtctcg tgaactgtat atcccgatgg gtgccgctct ggccatttac
      961 gctcgcgaga acggcgatgg tgtgatgttc gaaccagaag aaatctatga cgagctgaat
     1021 attggtagtg gaccggtcgc caccatggcc gagatcggca ccggcttccc cttcgacccc
     1081 cactacgtgg aggtgctggg cgagcgcatg cactacgttg acgtgggccc ccgcgacggc
     1141 acccccgtgc tgttcctgca cggcaacccc accagcagct acgtgtggcg caacatcatc
     1201 ccccacgtgg cccccaccca ccgcactatc gcccccgacc tgatcggcat gggcaagagc
     1261 gacaagcccg acctgggcta cttcttcgac gaccacgtgc gcttcatgga cgccttcatc
     1321 gaggccctgg gcctggagga ggtggtgctg gtgatccacg actggggcag cgccctgggc
     1381 ttccactggg ccaagcgcaa ccccgagcgc gtgaagggca tcgccttcat ggagttcatc
     1441 cgccccatcc ccacctggga cgagtggccc gagttcgccc gcgagacctt ccaggccttc
     1501 cgcaccaccg acgtgggccg caagctgatc atcgaccaga acgtgttcat cgagggcacc
     1561 ctgcccatgg gcgtggtgcg ccccctgacc gaggtggaga tggaccacta ccgcgagccc
     1621 ttcctgaacc ccgtggaccg cgagcccctg tggcgcttcc ccaacgagct gcccatcgcc
     1681 ggcgagcccg ccaacatcgt ggccctggtg gaggagtaca tggactggct gcaccagagc
     1741 cccgtgccca agctgctgtt ctggggcacc cccggcgtgc tgatcccccc cgccgaggcc
     1801 gcccgcctgg ccaagagcct gcccaacgtt aaggccgtgg acatcggccc cggcctgaac
     1861 ctgctccagg aggacaaccc cgacctgatc ggcagcgaga tcgcccgctg gctgagcacc
     1921 ctggagatca gcggcgtgta caagtccgga ctcagatctc gagaacagct gtcggatgcg
     1981 gataagctgc gcaaggtgat ctgtgaactg ctggagactg aacgcaccta tgtgaaagac
     2041 ttaaactgcc tcatggagag atacctgaag ccccttcaga aggagacctt tctcacccag
     2101 gatgagcttg atgtactttt tggaaattta accgaaatgg tggagtttca agtcgagttc
     2161 cttaagactc tagaagatgg agtaagactg gtccctgact tggaaaagct ggagaaggtt
     2221 gaccagttca agaaagtgct cttctctctg gggggatcct tcctgtacta cgcggaccgc
     2281 ttcaagctct acagtgcctt ctgtgcgagc cacacaaaag tccccaaggt cctggtgaaa
     2341 gccaagacgg acacagcctt caaggcgttc ctcgatgccc agaacccgag gcagcagcac
     2401 tcatccacgc tggagtctta cctcatcaag cccatccaga gggtcctcaa gtacccgctt
     2461 ctgctcaggg agctgtttgc gctgaccgat gcggagagcg aggaacacta ccacctggac
     2521 gtggccatca agactatgaa caaggtcgcc agtcacatca atgagatgca gaagatccac
     2581 gaagagtttg gtgccgtgtt tgaccagctg attgctgagc agacgggaga gaagaaagag
     2641 gttgcagatc tgagcatggg tgacctgctc ttgcacacca gcgtcatctg gctgaaccca
     2701 cccgcctcac tgggaaagtg gaaaaaggag ccagaattag cagccttcgt cttcaaaacg
     2761 gccgtggtcc ttgtatataa agacggttcc aagcagaaga agaaacttgt tggctctcac
     2821 aggctgtcaa tctacgagga gtgggacccc ttccggtttc gccacatgat ccctaccgaa
     2881 gctttgcagg tccgagctct gcccagtgca gatgcagagg caaatgcggt atgcgaaatt
     2941 gtccacgtga aatcagagtc agaagggagg cccgagcggg ttttccacct ctgctgcagt
     3001 tccccagaga gcagaaagga ctttctgaag tctgtgcatt cgatcctgcg agataaacac
     3061 agaagacagt gagcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt
     3121 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     3181 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gatcgacaat
     3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3601 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     3661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg cgatggtacc
     3841 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3901 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3961 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     4021 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4081 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4141 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     4201 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     4261 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     4321 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     4381 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     4441 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     4501 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     4561 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     4621 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacaattcga
     4681 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4741 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4801 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4861 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4921 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4981 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5041 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5101 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5161 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5221 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5281 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5341 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     5401 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5461 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5521 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5581 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5641 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5701 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5761 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5821 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5881 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5941 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6001 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6061 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6121 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6181 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6241 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6301 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6361 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6421 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6481 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6541 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6601 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6661 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6721 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6781 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6841 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6901 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6961 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7021 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7081 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7141 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7321 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7741 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7801 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7861 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7921 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7981 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8041 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8101 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8161 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8221 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     8281 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     8341 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     8401 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     8461 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8521 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8581 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8641 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8701 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8761 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8821 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8881 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8941 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9001 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9061 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9121 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     9181 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9241 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9301 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9361 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9421 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9481 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9541 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9601 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9661 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9721 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9781 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9841 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9901 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     9961 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
    10021 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10081 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10141 aaaaattcaa aattttatcg ataagcttgg gag
//
