LOCUS       sequence-346214-       10583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterodimerization with iLID, Cdc42 activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10583)
  AUTHORS   Mahlandt EK, Palacios Martinez S, Arts JJG, Tol S, van Buul JD, 
            Goedhart J
  TITLE     Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control
            Rho GTPase activity on a global to subcellular scale, enabling 
            precise control over vascular endothelial barrier strength.
  JOURNAL   Elife. 2023 Jul 14;12:RP84364. doi: 10.7554/eLife.84364.
  PUBMED    37449837
REFERENCE   2  (bases 1 to 10583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-07-14"; volume: "12:RP84364. doi"; pages: " 
            10.7554/eLife.84364"
COMMENT     Sequence Label: pLV-SspB-HaloTag-ITSN1-DHPH
FEATURES             Location/Qualifiers
     source          1..10583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             549..878
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     regulatory      894..903
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3101..3653
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3245..3262)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3472..3491
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3680..4279
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3680..3699)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4176..4196
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5171..5404
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5476..5597
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5513..5532)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5567..5586
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5637..5772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5792..5811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5793..5811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5821..5838)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5821..5837)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5830..5852)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5979..6434
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6066..6085)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6276..6297
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6460..6564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6565..6633,6634..7425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6783..6802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7596..8184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8085..8104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8338..8355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8472..8493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8508..8513,8514..8531,8532..8538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8546..8562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8551..8573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8570..8586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8570..8586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8605..8625
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8607..8625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8653..8879
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8880..9060
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9107..9232
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9725..9958
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10143..10187
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10449..10566
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      541 ccaccatgag ctccccgaaa cgccctaagc tgctgcgtga atattacgat tggctggttg
      601 ataacagctt taccccatat ctggtggtgg atgccacata cctgggcgtg aacgtgcccg
      661 tggagtatgt gaaagacggt cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc
      721 tgcaactgac aaatgatttt atccagttca acgcccgctt taagggcgtg tctcgtgaac
      781 tgtatatccc gatgggtgcc gctctggcca tttacgctcg cgagaacggc gatggtgtga
      841 tgttcgaacc agaagaaatc tatgacgagc tgaatattgg tagtggaccg gtcgccacca
      901 tggccgagat cggcaccggc ttccccttcg acccccacta cgtggaggtg ctgggcgagc
      961 gcatgcacta cgttgacgtg ggcccccgcg acggcacccc cgtgctgttc ctgcacggca
     1021 accccaccag cagctacgtg tggcgcaaca tcatccccca cgtggccccc acccaccgca
     1081 ctatcgcccc cgacctgatc ggcatgggca agagcgacaa gcccgacctg ggctacttct
     1141 tcgacgacca cgtgcgcttc atggacgcct tcatcgaggc cctgggcctg gaggaggtgg
     1201 tgctggtgat ccacgactgg ggcagcgccc tgggcttcca ctgggccaag cgcaaccccg
     1261 agcgcgtgaa gggcatcgcc ttcatggagt tcatccgccc catccccacc tgggacgagt
     1321 ggcccgagtt cgcccgcgag accttccagg ccttccgcac caccgacgtg ggccgcaagc
     1381 tgatcatcga ccagaacgtg ttcatcgagg gcaccctgcc catgggcgtg gtgcgccccc
     1441 tgaccgaggt ggagatggac cactaccgcg agcccttcct gaaccccgtg gaccgcgagc
     1501 ccctgtggcg cttccccaac gagctgccca tcgccggcga gcccgccaac atcgtggccc
     1561 tggtggagga gtacatggac tggctgcacc agagccccgt gcccaagctg ctgttctggg
     1621 gcacccccgg cgtgctgatc ccccccgccg aggccgcccg cctggccaag agcctgccca
     1681 acgttaaggc cgtggacatc ggccccggcc tgaacctgct ccaggaggac aaccccgacc
     1741 tgatcggcag cgagatcgcc cgctggctga gcaccctgga gatcagcggc gtgtacaagt
     1801 ccggactcag atctgatatg ttgaccccaa ctgaaagaaa gcgacaagga tacatccacg
     1861 agctcattgt caccgaggag aactatgtga atgacctgca gctggtcaca gagatttttc
     1921 aaaaacccct gatggagtct gagctgctga cagaaaaaga ggttgctatg atttttgtga
     1981 actggaagga gctgattatg tgtaatatca aactactaaa agcgctgaga gtccgcaaga
     2041 agatgtccgg ggagaagatg cctgtgaaga tgattggaga catcctgagc gcacagctgc
     2101 cgcacatgca gccctacatc cgcttctgca gccgccagct caacggggct gccctgatcc
     2161 agcagaagac ggacgaggcc ccagacttca aggagttcgt caaaagattg gcaatggatc
     2221 ctcggtgtaa agggatgcca ctctctagtt ttatactgaa gcctatgcaa cgggtaacaa
     2281 gatacccact gatcattaaa aatatcctgg aaaacacccc tgaaaaccac ccggaccaca
     2341 gccacttgaa gcacgccctg gagaaggcgg aagagctctg ttcccaggtg aacgaagggg
     2401 tgcgggagaa ggagaactct gaccggctgg agtggatcca ggcccacgtg cagtgtgaag
     2461 gcctgtctga gcaacttgtg ttcaattcag tgaccaattg cttggggccg cgcaaatttc
     2521 tgcacagtgg gaagctctac aaggccaaga gcaacaagga gctgtatggc ttccttttca
     2581 acgacttcct cctgctgact cagatcacga agcctttggg gtcttctggc accgacaaag
     2641 tcttcagccc caaatcaaac ctgcagtata aaatgtataa aacacctatt ttcctaaatg
     2701 aggttctagt aaaattaccc accgaccctt ctggagacga gcccatcttc cacatctccc
     2761 acattgaccg cgtctatact ctccgagcag aaagcataaa tgaaaggact gcctgggtgc
     2821 agaaaatcaa agctgcttct gaactctaca tagagactga gaaaaagaag taagaattct
     2881 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gcctagtgcc atttgttcag
     2941 tggttcgtag ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg
     3001 gggccaagtc tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt
     3061 ctttgggtaa acccgggaat tccgcccctc tcccccccta acgttactgg ccgaagccgc
     3121 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     3181 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     3241 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     3301 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     3361 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     3421 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     3481 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     3541 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     3601 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggatat
     3661 ggccacaacg gtcgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3721 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3781 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3841 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3901 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3961 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     4021 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     4081 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     4141 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     4201 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     4261 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     4321 acgaccccat gcatgtattc aatctaagca ggctttcact ttctcgccaa cttacaaggc
     4381 ctttctgtgt aaacaatacc tgaaccttta ccccgttgcc cggcaacggc caggtctgtg
     4441 ccaagtgttt gctgacgcaa cccccactgg ctggggcttg gtcatgggcc atcagcgcat
     4501 gcgtggaacc ttttcggctc ctctgccgat ccatactgcg gaactcctag ccgcttgttt
     4561 tgctcgcagc aggtctggag caaacattat cgggactgat aactctgttg tcctatcccg
     4621 caaatataca tcgtttccat ggctgctagg ctgtgctgcc aactggatcc tgcgcgggac
     4681 gtcctttgtt tacgtcccgt cggcgctgaa tcctgcggac gacccttctc ggggtcgctt
     4741 gggactctct cgtccccttc tccgtctgcc gttccgaccg accacggggc gcacctctct
     4801 ttacgcggac tccccgtctg tgccttctca tctgccggac cgtgtgcact tcgcttcacc
     4861 tctgcacgtc gcatggagac caccgtgaac gcccaccaaa tattgcccaa ggtcttacat
     4921 aagaggactc ttggactctc agcaatgtca acgaccgacc ttgaggcata cttcaaagac
     4981 tgtttgttta aagactggga ggagttgggg gaggagatta ggttaaaggt ctttgtacta
     5041 ggaggctgta ggcataaatt ggtctgcgca ccagcaccat ggcgcaatca ctagagcggg
     5101 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5161 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5221 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5281 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5341 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5401 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     5461 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5521 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5581 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     5641 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     5701 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     5761 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     5821 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     5881 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     5941 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     6001 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6061 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6121 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6181 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6241 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6301 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6361 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     6421 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     6481 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     6541 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     6601 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     6661 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     6721 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     6781 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     6841 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     6901 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     6961 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     7021 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     7081 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     7141 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     7201 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     7261 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     7321 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     7381 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     7441 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     7501 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7561 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7621 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7681 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7741 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7801 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7861 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7921 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7981 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     8041 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     8101 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     8161 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     8221 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     8281 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     8341 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     8401 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     8461 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     8521 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     8581 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     8641 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     8701 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     8761 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     8821 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     8881 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     8941 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     9001 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     9061 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     9121 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     9181 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     9241 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     9301 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     9361 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     9421 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     9481 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     9541 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     9601 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     9661 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     9721 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     9781 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     9841 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     9901 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     9961 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    10021 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
    10081 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    10141 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
    10201 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
    10261 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
    10321 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
    10381 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
    10441 ggtatcggtt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10501 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10561 aattttatcg ataagcttgg gag
//
