LOCUS       sequence-346215-        2385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site XII-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-XII5dw
FEATURES             Location/Qualifiers
     source          1..2385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     473..492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1423..1457)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1528..2187)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2102..2121
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2369..2385,1..572)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
       61 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      661 tggataaccg tagtcggtct cagagtcctg caggactagt gctgaggcat taatgcaact
      721 cagaagtttg acagcaagca agttcatcat tcgaactagc cttattgttt tagttcagtg
      781 acagcgaact gccgtactcg atgctttatt tctcacggta gagcggaaga acagataggg
      841 gcagcgtgag aagagttaga aagtaaattt ttatcacgtc tgaagtattc ttattcatag
      901 gaaattttgc aaggtttttt agctcaataa cgggctaagt tatataaggt gttcacgcga
      961 ttttcttgtt atgtatacct cttctctgag gaatggtact actgtcctga tgtaggctcc
     1021 ttaaattggt gggcaagaat aacttatcga tattttgtat attggtcttg gagttcacca
     1081 cgtaatgcct gtttaagacc atcagttaac tctagtatta tttggtcttg gctactggcc
     1141 gtttgctatt attcaagtct tttgtgcctt cccgtcgggt aagggagtta tttagggata
     1201 cagaatctaa cgaaaactaa atctcaatga ttaactctat ttaatccttt tttgaaaggc
     1261 aaaagaggtc ccttgttcac ttacaacgtt cttagccaaa ttcgcttatc acttactact
     1321 tcacgatata cagaagtaaa aacatataaa aagatgtctg tttgtttagc catcacaaaa
     1381 ggtatcgcag attaagacct cagcgccgat gagaccagac caataaaaaa cgcccggcgg
     1441 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     1501 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     1561 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     1621 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1681 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1741 gggattggct gaaacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1801 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     1861 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     1921 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     1981 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2041 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2101 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2161 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2221 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2281 gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2341 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttc
//
