LOCUS       sequence-346216-        2157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site XII-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-XII5up
FEATURES             Location/Qualifiers
     source          1..2157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     344..363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1066..1100)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1171..1830)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1745..1764
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2012..2157,1..443)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
       61 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
      121 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
      181 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      241 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      301 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      361 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      421 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      481 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      541 gtagtcggtc tcacaatgcg ctgagggttt aatcaatctg gcggcttgag ttctcaacat
      601 gttttatttt ttacttatat tgctggtagg gtaaaaaaat ataactccta ggaataggtt
      661 gtctatatgt ttttgtcttg cttctataat tgtaacaaac aaggaaaggg aaaatactgg
      721 gtgtaaaagc cattgagtca agttaggtca tcccttttat acaaaatttt tcaatttttt
      781 ttccaagatt cttgtacgat taattatttt ttttttgcgt cctacagcgt gatgaaaatt
      841 tcgcctgctg caagatgagc gggaacgggc gaaatgtgca cgcgcacaac ttacgaaacg
      901 cggatgagtc actgacagcc accgcagagg ttctgactcc tactgagctc tattggaggt
      961 ggcagaaccg gtaccggagg aggccgctat aaccggtttg aatttattgt cacagtgtca
     1021 catcagcatt aagtcctcag cgagctcgcc cttgagacca gaccaataaa aaacgcccgg
     1081 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     1141 aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact catcgcagta
     1201 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     1261 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     1321 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     1381 ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga aataggccag
     1441 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     1501 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     1561 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg
     1621 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     1681 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     1741 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     1801 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     1861 taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct
     1921 tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactg
//
