LOCUS       sequence-34622-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TDRD3 (3PMT)
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             288..305
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(358..376)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      372..419
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      456..836
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(543..562)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     753..774
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca cagcagcggc
       61 agagaaaact tgtatttcca gggcaaaatg tggaaacctg gagatgaatg ttttgcactt
      121 tattgggaag acaacaagtt ttaccgggca gaagttgaag ccctccattc ttcgggtatg
      181 acagcagttg ttaaattcat tgactacgga aactatgaag aggtgctact gagcaatatc
      241 aagcccattc aaacagagtg atgacgaagc ttgcggccgc actcgagcac caccaccacc
      301 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
      361 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
      421 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
      481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
      661 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
      721 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
      781 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
      841 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg a
//
