LOCUS       sequence-346220-        2284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site XI-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-XI1dw
FEATURES             Location/Qualifiers
     source          1..2284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     311..330
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      578..1166
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1067..1086
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1916..1950)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(2021..2284,1..396))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
       61 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
      121 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
      181 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
      241 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
      301 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
      361 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
      421 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      481 acctcttacg tgcccgatca atcatgacca aaatccctta acgtgagttt tcgttccact
      541 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      601 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
      661 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
      721 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      781 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
      841 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
      901 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
      961 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1021 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1081 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1141 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1201 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1261 accgtagtcg gtctcagagt cctgcaggac tagtgctgag gcattaatag tttcttggca
     1321 ttggcaaatc tctgctaaat gctgcgtaca gacggaaact cacaccgccg cgaaaactgg
     1381 tcagtggcaa aaaaaaaaaa attaaaaaaa taaaaaataa ctattacgta tgatactgtt
     1441 tctggtagtt gatatgaggt tggtgttgta tattgtacgt tttaggaaca gggaagtgaa
     1501 tattatttac tctgctgcac attctggcta ggtcgaagcc ggaacctgag atgacgccgc
     1561 gctagaacta tggaccaagc tgttgacaat gtttagatgg tgatgcacta ccctgtgcgg
     1621 ggagtggcca cgaacgcgag cggaaggtgc gggtgttgcg ggaattgcgg gaggttcttc
     1681 gctaagcgtg agggttgcta gttggggcgg cggggtttcc ctaagtgtaa atagggcctc
     1741 gccgctggca catgagtgcg ccggaggagg cggcggaggc gacgacgcta aaaccgtggc
     1801 cgttggggaa ggatgggcgg ctatatctac cattgacctg atggggactc ggctcttaag
     1861 gaatgggttt gaggtgggtg tggattaaga cctcagcgcc gatgagacca gaccaataaa
     1921 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1981 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     2041 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     2101 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2161 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2221 tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac cctttaggga
     2281 aata
//
