LOCUS       sequence-346221-       10061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterodimerization with FRB (rapamycin), Rac1 activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Mahlandt EK, Palacios Martinez S, Arts JJG, Tol S, van Buul JD, 
            Goedhart J
  TITLE     Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control
            Rho GTPase activity on a global to subcellular scale, enabling 
            precise control over vascular endothelial barrier strength.
  JOURNAL   Elife. 2023 Jul 14;12:RP84364. doi: 10.7554/eLife.84364.
  PUBMED    37449837
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-07-14"; volume: "12:RP84364. doi"; pages: " 
            10.7554/eLife.84364"
COMMENT     Sequence Label: pLV-mCherry-FKBP-TIAM1(DHPH)
FEATURES             Location/Qualifiers
     source          1..10061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    630..654
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             678..1385
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(823..841)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1091..1110
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1398..1718
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     protein_bind    complement(2984..3008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3061..3102
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3126..3714
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3179..3199)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3597..3608)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3729..4045
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3896..4031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4093..4140
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4159..4557
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4644..4877
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4949..5070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4986..5005)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5040..5059
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5110..5245
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5265..5284)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5266..5284)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5294..5311)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5294..5310)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5303..5325)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5452..5907
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5539..5558)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5749..5770
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5933..6037
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6038..6106,6107..6898)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6256..6275)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7069..7657
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7558..7577
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7811..7828
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7945..7966
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7981..7986,7987..8004,8005..8011)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8019..8035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8024..8046
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8043..8059
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8043..8059
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8078..8098
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8080..8098
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8126..8352
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8353..8533
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8580..8705
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9198..9431
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9616..9660
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9927..10044
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagag gatccactag
      601 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctttaaag
      661 gaaccaattc agtcgacatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
      721 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
      781 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
      841 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
      901 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
      961 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     1021 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     1081 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     1141 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     1201 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     1261 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     1321 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     1381 acaagagtgc tggtggtgga gtgcaggtgg aaaccatctc cccaggagac gggcgcacct
     1441 tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa gatggaaaga
     1501 aatttgattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc aagcaggagg
     1561 tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga gccaaactga
     1621 ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc ccaccacatg
     1681 ccactctcgt cttcgatgtg gagcttctaa aactggaaag tgctggtggt agtgctggtg
     1741 gtagtgctgg tggtagtgct ggtggtagtg ctggtggtcc tcgagcaatg aacccctctg
     1801 accagagccc atctcctcag gactccacgg ggcctcagct ggcgaccatg agacaactct
     1861 cggatgcaga taagctgcgc aaggtgatct gcgagctcct ggagacggag cgcacctacg
     1921 tgaaggattt aaactgtctt atggagagat acctaaagcc tcttcaaaaa gaaacttttc
     1981 tcacccagga tgagcttgac gtgctttttg gaaatttaac ggaaatggta gagtttcaag
     2041 tagaattcct taaaactcta gaagatggag tgagactggt acctgatttg gaaaagcttg
     2101 agaaggttga tcaatttaag aaagtgctgt tctctctggg gggatcattc ctgtattatg
     2161 ctgaccgctt caagctctac agtgccttct gcgccagcca cacaaaagtt cccaaggtcc
     2221 tggtgaaagc caagacagac acggctttca aggcattctt ggatgcccag aacccgaagc
     2281 agcagcactc atccacgctg gagtcgtacc tcatcaagcc catccagagg atcctcaagt
     2341 acccacttct gctcagggag ctgttcgccc tgaccgatgc ggagagcgag gagcactacc
     2401 acctggacgt ggccatcaag accatgaaca aggttgccag tcacatcaat gagatgcaga
     2461 aaatccatga agagtttggg gctgtgtttg accagctgat tgctgaacag actggtggga
     2521 aaaaagaggt tgcagatctg agcatgggag acctgctttt gcacactacc gtgatctggc
     2581 tgaacccgcc ggcctcgctg ggcaagtgga aaaaggaacc agagttggca gcattcgtct
     2641 tcaaaactgc tgtggtcctt gtgtataaag atggttccaa acagaagaag aaacttgtag
     2701 gatctcacag gctttccatt tatgaggact gggacccctt cagatttcga cacatgatcc
     2761 ccacggaagc gctgcaggtt cgagctttgg cgagtgcaga tgcagaggca aatgccgtgt
     2821 gtgaaattgt ccatgtaaaa tccgagtctg aagggaggcc ggagagggtc tttcacttgt
     2881 gctgcagctc cccagagagc cgaaaggatt tcctaaaggc tgtgcattca atcctgcgtg
     2941 ataagcacag aagacagtga gcggccgcac tcgagatatc tagacccagc tttcttgtac
     3001 aaagtggttg atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
     3061 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga
     3121 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     3181 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3241 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3301 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3361 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3421 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3481 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3541 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3601 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3661 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggcg
     3721 atggtaccgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3781 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3841 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3901 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3961 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     4021 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     4081 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     4141 aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
     4201 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
     4261 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
     4321 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     4381 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     4441 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     4501 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca
     4561 caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     4621 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     4681 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     4741 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     4801 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     4861 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     4921 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     4981 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     5041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     5101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5221 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     5281 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     5341 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     5401 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     5461 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     5521 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     5581 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     5641 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     5701 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     5761 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     5821 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     5881 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     5941 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6001 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6061 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6121 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6181 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6241 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6301 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6361 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6421 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6481 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6541 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6601 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6661 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6721 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6781 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6841 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6901 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6961 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7741 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7801 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7861 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7921 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7981 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8041 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     8101 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     8161 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     8221 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     8281 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     8341 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8401 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8461 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8521 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     8581 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     8641 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     8701 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     8761 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     8821 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     8881 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     8941 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     9001 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     9061 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     9121 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     9181 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     9241 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     9301 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     9361 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     9421 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     9481 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     9541 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     9601 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9661 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9721 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9781 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     9841 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     9901 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     9961 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
    10021 caaattacaa aaattcaaaa ttttatcgat aagcttggga g
//
