LOCUS       sequence-346222-        2164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site XI-5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-XI5dw
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      134..722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     623..642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1352..1386)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1457..2116)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2031..2050
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca
       61 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      121 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      181 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      241 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      301 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      361 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      421 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      481 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      541 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      601 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      661 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      721 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      781 tgttctttcc tgcgttatcc cctgattctg tggataaccg tagtcggtct cagagtcctg
      841 caggactagt gctgaggcat tactattggc tgcttcatag tacacccaat tgcccacaac
      901 ccaagttaat cataagctct tgttgtcgat gaattgctca aaatgtggcc attctttcaa
      961 ttttgcacct ctaaaactgc tccgtgggtt gtgacaacaa ccttaagggt aatggacttt
     1021 tttagaaaga caaatgagat gatttcacct actggtatat catttattta tacacgaccg
     1081 ttttgacatt tcagagttgc acgttggtat atcctaatat tctcacaagc atcacagtga
     1141 agaaacgcaa gaaaatggaa aaaattccca ggtggttgtt gtcttctctt atatcgtcgg
     1201 tgctgtgcat acttggggcc ctgtgtgtgc cgttgttatc ggttgccttc gatagcaagc
     1261 gcaatagcca atctaagttg gtcaactatg gtctttctct aagtgccgga tctatgatca
     1321 ttaagacctc agcgccgatg agaccagacc aataaaaaac gcccggcggc aaccgagcgt
     1381 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     1441 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     1501 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     1561 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     1621 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     1681 aaacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     1741 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     1801 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     1861 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     1921 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     1981 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     2041 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     2101 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     2161 cgcc
//
