LOCUS       sequence-346223-        2199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site X-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-X2dw
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     363..382
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      630..1218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1119..1138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1883..1917)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1988..2199,1..448))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
       61 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
      121 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
      181 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
      241 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
      301 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
      361 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
      421 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
      481 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
      541 cgtgcccgat caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtagt
     1321 cggtctcaga gtcctgcagg actagtgctg aggcattaat cctgcataat cggcctcaca
     1381 gagggatccc gttacccatc tatgctgaag atttatcata ctattcctcc gctcgtttct
     1441 tttttcagtg aggtgtgtcg tgaaagaaaa cccacaatta aactttcaac aaccgggcga
     1501 ctaggaagag agtagtgggc gcggatgacg aaggctaagg tcacttcttc gtttccttta
     1561 ttggggtttc cgtgtagcct tcccctgaat agtgtgggac gttttatgag aagccgtaag
     1621 aaataggcaa attgagttat gacaagtaga catgatgccg cagccttgcc tgactttacg
     1681 actccttcat gaataagttt ttctatcgag ttcttttcct tttttcgcct taattagctc
     1741 aattaagcct gtcctcacta cttttctttt tcttatcggc tttgtgccac acctaacctt
     1801 cgaatgctgt tttattccgt tcttacatgg gatggtaatg ccttggcgag attaagacct
     1861 cagcgccgat gagaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     1921 atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga
     1981 tatcaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
     2041 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
     2101 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
     2161 tattggccac gtttaaatca aaactggtga aactcaccc
//
