LOCUS       sequence-346225-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site XI-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-XI3up
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     384..403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1227..1261)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1332..1991)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1906..1925
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2173..2278,1..483)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
       61 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      121 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      181 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      241 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      301 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      361 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      421 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      481 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      541 atgttctttc ctgcgttatc ccctgattct gtggataacc gtagtcggtc tcacaatgcg
      601 ctgagggttt aatagttact tgctctatgc gtttgcgcat cctcttttta cttttttttt
      661 ttcagtaaag cctaagcata aatcgtttta tacgtacgac acgttcaact tttcttggtt
      721 agtagtggca atctctgcaa tacatacagg gagtcatggt ctatcatctt gtccaatcaa
      781 agaagcatcg gttcagatcg agcaaactgt agggagaaag gaaagtagaa atgcagagtg
      841 tgctatatgt ccaatctcgg ttttgtagtt tggatgtcat tagagatcta ccacccaacc
      901 ggctgctttc atgtggaaca gaaaagaaat cggggcgctt cctcttctgt attcctttag
      961 ttaacgtttt tattcagcca tctaaccatc atacccccat acggtaacaa aacctcttct
     1021 aagaaaagaa gtctctgctc ctccgccatc ttatttttat tcgctgcgcg cgtttattgt
     1081 cgcatcgctg gccagcaaaa aattggttgc ctttttttac ctaaaaaaga cacatctaac
     1141 tgattagttt tccgttttag gatattgacg ccaagcgtgc gtctgattat taagtcctca
     1201 gcgagctcgc ccttgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga
     1261 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc
     1321 tcgatatcaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     1381 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     1441 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     1501 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg
     1561 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     1621 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     1681 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     1741 atcaccagct caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg
     1801 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     1861 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     1921 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     1981 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     2041 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat
     2101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccg
//
