LOCUS       sequence-346226-        2272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site XI-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-XI2dw
FEATURES             Location/Qualifiers
     source          1..2272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     206..225
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      473..1061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     962..981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1799..1833)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1904..2272,1..291))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
       61 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      121 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      181 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      241 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      301 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      361 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
      421 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      481 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      541 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      601 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      661 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      721 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      781 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      841 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      901 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      961 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1021 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1081 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1141 ctgattctgt ggataaccgt agtcggtctc agagtcctgc aggactagtg ctgaggcatt
     1201 aatcacacga ctagcgcttt cagatattaa aaagtttaga tgtaggtttt agcggtaaca
     1261 gttatataaa tcgtgtttct tctcttgatg aaacaaaaaa atgctagaaa aactttgtcg
     1321 tttcttactt ttggtgcgct ttgcagtttt cgtggctaga cttagaatca tttctcctca
     1381 gatttcttga ttaaagtttg gtgcgaagcc ctactctaac attggtgttc ttcttttcat
     1441 tcacgcaagt taagtccagg aaggtgagca aatgctcatc cttctgttca tgcgtgacgg
     1501 ctgaattatc cttatctggc gtacccgtgc agccgtttcc gtgcctcggt tcctccgaga
     1561 tatccttagg gaccgccagg gaccatgatt gcgtcaactg ttgtcaccgc tccagaggat
     1621 cctctgtaac cttttcaacc ataaaaatag aaagggcaca gggttggggt atttgaattt
     1681 tttttttggg ttttttcgta ttacttatta cttaccttct tttctatata attttgtttt
     1741 tccctggtag agcggaatct tcccacatta agacctcagc gccgatgaga ccagaccaat
     1801 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1861 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1921 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1981 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     2041 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     2101 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
     2161 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     2221 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tg
//
