LOCUS       sequence-346227-        2164 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site XI-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-XI1up
FEATURES             Location/Qualifiers
     source          1..2164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     384..403
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      651..1239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1140..1159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1869..1903)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1974..2164,1..469))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc tcaataaacc
       61 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
      121 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
      181 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
      241 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
      301 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      361 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      421 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
      481 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      541 ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag
      601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1261 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1321 gattctgtgg ataaccgtag tcggtctcac aatgcgctga gggtttaatg gaatagtgac
     1381 gttgtgatgc ggtgagttcg gcggttaggg gaatggtata tgataaaaaa cggaaacgtg
     1441 cttctttaat ttaattgttt aatattgttg cagatatata aaaaggggga aagaaccgaa
     1501 gatgtaatta tttttttatc gcctcaacct aaagcaagca ataaggtata aagatcagga
     1561 cgactcgagc gctgatatct aaatttgaag ccacgcaagt aactacgtag gtcagagggg
     1621 acaaggaata acacttgaca tttttctttt ttcttttttt ttcttttttt tttttttgtt
     1681 aatcttggct tctgtaccgt agcctcctgt atacggtttt agatgcagta tgtttatcat
     1741 cgccgagaaa tttggggggt gcaaaggaat aaaatccgtg ctgaaatccg tgcaccgcat
     1801 caaactttct cggaggattc tttgcagccg attaagtcct cagcgagctc gcccttgaga
     1861 ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt
     1921 ctgaggtcat tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc
     1981 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     2041 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     2101 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     2161 aaat
//
