LOCUS       sequence-346228-        2155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 7 containing 3' homology for 
            integration site X-4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC7-X4dw
FEATURES             Location/Qualifiers
     source          1..2155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     252..271
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      519..1107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1008..1027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1728..1762)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1833..2155,1..337))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
       61 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
      121 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
      181 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
      241 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
      301 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
      361 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
      421 aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt ttcgttccac
      481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
      781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
      841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
      901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1201 aaccgtagtc ggtctcagag tcctgcagga ctagtgctga ggcattaata catgggaaga
     1261 ttcgcttttt ttttttgaat tacaatagta tgtctgatgt ctgcaagaag taacaggcgt
     1321 gtgcacaaga atacgtgtgt gtgcgtaagc gtatgcactg gtggcataac ttatctaaga
     1381 agtatatatc actgacatag aaatgtagat atacaggtat ttttctcgat aatcgataaa
     1441 aatctcgtcg cgctgaacca aacttggtgg ttacggagag tttttctctc atcattactg
     1501 tctttcgcat tgatttcccc tttgaccgat aaaatccctt ggattcataa gattaaacaa
     1561 agaggtgatc aaagagaacc ctgtgaaagt ttatgtttat aaccgggcat aaagtgaact
     1621 agacactttc aagaagccaa ccaaagcatg agtaacgaag cttaccagca tgatcatacc
     1681 gtaaatcctc accagattaa gacctcagcg ccgatgagac cagaccaata aaaaacgccc
     1741 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1801 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     1861 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     1921 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     1981 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     2041 acccagggat tggctgaaac gaaaaacata ttctcaataa accctttagg gaaataggcc
     2101 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccgga
//
