LOCUS       sequence-346229-        2238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site XII-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-XII2up
FEATURES             Location/Qualifiers
     source          1..2238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     266..285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1069..1103)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1174..1833)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1748..1767
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(2015..2238,1..365)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
       61 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      421 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg tctcacaatg
      481 cgctgagggt ttaatcggca tgcaaacatc tacacaatta gcaagggcaa tccatatttt
      541 gtcttttcgc gccctggaaa ggcctaagta atgtcgtaaa cgcattctat ctgtacttca
      601 actctcctct gtgcattggt ttgtgcaaat cacattttac gatactgcca gatatatgca
      661 aaaagagaaa accaagggac cagaacaaag caaaattacg atattcttcg aattccttcg
      721 tgcttgacta agacaaaggg atggacgtag cgatttttag cgggccaaga actggttccg
      781 aaaaagcaca ggtacaccga accctcagct aaggagggac agcaccgatg cggaaggaca
      841 aactttcttt ttgcctatca cagtatctta tcgagctaac tattttcgac acacatgaaa
      901 aagcagaaat attaacgaaa aagaaaagaa agaccatgtc atgtacgggc aatcagaatc
      961 tgtaacaagc gccatttttt tttctgtatc gggccctcct tactgctctc cttccgtgta
     1021 acgcgttatg attaagtcct cagcgagctc gcccttgaga ccagaccaat aaaaaacgcc
     1081 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     1141 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     1201 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
     1261 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     1321 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     1381 cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag ggaaataggc
     1441 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     1501 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     1561 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     1621 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     1681 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     1741 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     1801 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     1861 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     1921 tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2221 acctcgctct gctaatcc
//
