LOCUS       sequence-346230-        2234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 8b containing 5' homology for 
            integration site X-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC8b-X2up
FEATURES             Location/Qualifiers
     source          1..2234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     323..342
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      590..1178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1079..1098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1878..1912)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(1983..2234,1..408))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
       61 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
      121 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
      181 tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
      241 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
      301 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
      361 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
      421 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      481 gtgaaagttg gaacctctta cgtgcccgat caatcatgac caaaatccct taacgtgagt
      541 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      601 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      661 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      721 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      781 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      841 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      901 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      961 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1021 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1081 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1141 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1201 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1261 attctgtgga taaccgtagt cggtctcaca atgcgctgag ggtttaatcg tctatgagga
     1321 gactgttagt tggatatcag taatgagtcg aaaaagctcg aaatgaatgg atatattctt
     1381 tttgctactg gcaactgttg aatatttaat gttaaaacaa actaactgag gtatattcgt
     1441 atctgtatgt acacatatac tatatacagg aaaagataag caagagagag gatatcaact
     1501 acgagagcga tcgattatat atcaaaagct gtccgctttg ccacccataa tcggcgctta
     1561 gtttcggagt tcaatcataa ttctaccacc ttacactcaa cttactcttt aactcctata
     1621 gtataatatc gccactgacc ccatattaaa aaattttttt gctcgatctt ctatcctctt
     1681 taggttaatt gtcgctgtta ttgtctagat tttttctcgg agatggcgca tctatttgcc
     1741 gtcaaaagat cctctcatac catattaagt aaattgcctc catttctttt tcctcgggca
     1801 gagaaactcg caggcaactt gctctcgaag tggtcacgta ttaagtcctc agcgagctcg
     1861 cccttgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1921 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1981 aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg
     2041 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     2101 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     2161 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata
     2221 ttctcaataa accc
//
