LOCUS       sequence-346231-        2379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast MoClo level-0 part plasmid type 3 containing yeast-enhanced 
            GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F
  TITLE     Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based 
            Applications, Genomic Integrations and Combinatorial Libraries.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 
            10.1021/acssynbio.1c00408. Epub 2021 Dec 3.
  PUBMED    34860007
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00408"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3461-3474"
COMMENT     Sequence Label: pMC3-yeGFP
FEATURES             Location/Qualifiers
     source          1..2379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(7..41)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(112..771)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     686..705
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      953..1541
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1442..1461
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1653..2366
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
       61 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc
      121 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
      181 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
      241 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
      301 atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat tctcaataaa
      361 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
      421 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
      481 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
      541 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
      601 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      661 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      721 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
      781 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
      841 atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc ccttaacgtg
      901 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
      961 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     1021 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     1081 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1141 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1201 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1261 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1321 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1381 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1441 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1501 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1561 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1621 ctgattctgt ggataaccgt agtcggtctc atatgtctaa aggtgaagaa ttattcactg
     1681 gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa ttttctgtct
     1741 ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt atttgtacta
     1801 ctggtaaatt gccagttcca tggccaacct tagtcactac tttcggttat ggtgttcaat
     1861 gttttgcgag atacccagat catatgaaac aacatgactt tttcaagtct gccatgccag
     1921 aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac aagaccagag
     1981 ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa ggtattgatt
     2041 ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac tctcacaatg
     2101 tttacatcat ggctgacaaa caaaagaatg gtatcaaagt taacttcaaa attagacaca
     2161 acattgaaga tggttctgtt caattagctg accattatca acaaaatact ccaattggtg
     2221 atggtccagt cttgttacca gacaaccatt acttatccac tcaatctgcc ttatccaaag
     2281 atccaaacga aaagagagat cacatggtct tgttagaatt tgttactgct gctggtatta
     2341 cccatggtat ggatgaattg tacaaaggat cctgagacc
//
