LOCUS       sequence-346241-       17786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express internally GFP-tagged VPS13D in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17786)
  AUTHORS   Baldwin HA, Wang C, Kanfer G, Shah HV, Velayos-Baeza A, 
            Dulovic-Mahlow M, Bruggemann N, Anding A, Baehrecke EH, Maric D, 
            Prinz WA, Youle RJ
  TITLE     VPS13D promotes peroxisome biogenesis.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212018. doi: 
            10.1083/jcb.202001188.
  PUBMED    33891012
REFERENCE   2  (bases 1 to 17786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212018. doi"; 
            pages: " 10.1083/jcb.202001188"
COMMENT     Sequence Label: pVPS13D-I-EGFP
FEATURES             Location/Qualifiers
     source          1..17786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(9099..9101,9102..9104,9105..9815)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9144..9165)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9405..9424)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9752..9773
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    14514..14635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14551..14570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14605..14624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(14642..15097)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14779..14800)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14991..15010
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        15124..15228
                     /gene="bla"
                     /label=AmpR promoter
     promoter        15230..15587
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        15230..15421
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(15253..15273)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      15438..15573
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             15622..16416
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             15622..16416
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(15676..15695)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     16286..16305
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(16604..16623)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    16648..16695
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      17024..17612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     17513..17532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
      541 ccatgttgga aggccttgta gcctgggttc tcaataccta tttgggaaaa tatgtcaata
      601 acctgaacac tgaccagctc tcagttgcac ttctcaaagg tgctgttgaa ttagaaaact
      661 tgccattaaa gaaagatgcc ttgaaagaat tggaattacc atttgaagtc aaagctggct
      721 tcattgggaa agtaaccctt cagattccct tttatcgccc ccatgtggac ccttgggtga
      781 tctccatctc cagccttcac ttaattggag ccccagagaa aatacaggat ttcaatgatg
      841 aaaaggagaa gctgttggaa agggaacgta agaaagcact acttcaagcc ctggaggaga
      901 aatggaagaa tgaccgccag cagaaagggg agtcctattg gtattcagtt accgcctccg
      961 tagttacaag gattgtggag aatattgaat taaaaattca agatgtccat ttacgctttg
     1021 aagatggtgt caccaatccc tcccatcctt ttgcttttgg catctgcatt aagaatgtgt
     1081 ccatgcaaaa tgctgtgaat gagcctgtac agaaactaat gcggaaaaag caattagacg
     1141 tagcagaatt tagcatctat tgggatgtcg attgcacttt actgggggat ttgcctcaga
     1201 tggagttaca ggaggccatg gccaggagca tggagagtcg cagccatcac tacgtcctgg
     1261 agcctgtgtt tgcatctgct cttttgaaga gaaactgctc caagaagccc ctgcggtctc
     1321 ggcacagtcc ccgtattgat tgtgatattc agctggagac cattcccttg aaactctctc
     1381 agctgcaata ccggcaaatc atggaattcc tcaaggagct ggaacgaaag gagaggcagg
     1441 tgaagttccg aaggtggaaa cccaaggtgg cgatatctaa gaactgccga gaatggtggt
     1501 attttgcttt gaatgctaac ttgtatgaga tcagagagca gaggaaacgt tgcacctggg
     1561 actttatgtt gcaccgcgct cgtgatgctg tatcttacac tgacaaatat ttcaacaagt
     1621 taaaaggagg cctgctgtcc acagatgaca aggaggaaat gtgtcggatt gaagaggaac
     1681 agagctttga ggaattgaag attttgcgtg aactggttca tgatcgattt cacaaacagg
     1741 aagaactagc agagagtctg cgggagcctc agtttgattc tccaggagcc tgtccgggag
     1801 ccccagaacc cggtggaggc agtgggatgc tgcagtatct ccagtcctgg tttcctggat
     1861 ggggtggctg gtacgggcag cagaccccag aagggaatgt ggttgaggga ctgtcagcag
     1921 agcaacagga gcagtggatt cctgaagaga tcctgggcac tgaggagttt tttgacccca
     1981 ctgcagatgc ctcgtgtatg aacacgtata caaagcgaga tcatgtcttt gccaaactga
     2041 atttgcagtt gcagcgaggt acagtgactc tgttacacaa ggagcaagga actcctcaaa
     2101 tgaatgaaag tgctttcatg cagctcgagt tttcagatgt aaaacttcta gcagagtctc
     2161 ttcctcgaag aaattcctcg ttgctttcag tccggttggg tggactgttt cttcgagacc
     2221 tggctacaga aggaactatg tttcctcttc tagtcttccc taatccacaa aaagaagttg
     2281 gcagagtctc acaatctttt ggtctacaaa ctacatctgc agacagaagt gatcattacc
     2341 cagctgcaga tccagatggc cccgtttttg agatgctgta tgagagaaat ccggcgcaca
     2401 gccactttga gaggcggctc aatgtcagca caaggccctt gaacatcata tacaatccgc
     2461 aggccattaa aaaagtagca gactttttct acaagggaaa ggttcatacc tcaggttttg
     2521 gttatcagtc tgaacttgag ctgagagtgg ctgaagctgc ccgaagacaa tataacaagc
     2581 tgaagatgca gaccaaggca gaaatccggc aaactcttga tcgtttgcta gtgggtgatt
     2641 tcattgagga gagtaaacga tggaccgtgc ggctggatat ttctgcccct caggtgatat
     2701 ttcctgatga tttcaaattc aagaatcctg tgttagttgt cgtggatcta ggaagaatgc
     2761 ttttgacgaa cacccaagat aactccagga ggaaaagtag ggatgggtca gcatctgaag
     2821 agacccagtt tagtgatgat gaatataaga cccccctggc cacacctcct aacaccccac
     2881 ctcccgagtc aagcagcagc aacggagaga aaacacctcc cttttctgga gttgagttca
     2941 gtgaagaaca gcttcaagca catttaatga gcacaaagat gtatgagagg tactcgctgt
     3001 catttatgga cctccagatc atggttggac gagtgaaaga caattggaag catgtccagg
     3061 atattgacgt gggaccaaca catgtggtag agaagttcaa cgttcaccta cagttagagc
     3121 gtcgattgat ttatacttca gatcccaaat atccaggagc cgtgctctca ggcaacttac
     3181 cagacttaaa aatccacatt aatgaagata aaatatctgc actaaagaat tgctttgctc
     3241 tcctcaccac cccagaaatg aaaacttctg acactcagat taaagaaaag atttttcccc
     3301 aggaggagca gcggggaagt ttgcaagact ccgtaatgaa tttaacccag agcattgtgt
     3361 tgttggagca gcatacccgc gaggttctgg tggagtcgca gctcctcctg gcggaattta
     3421 aagtgaactg tatgcagctt ggtgttgaga gcaatggccg gtacatttct gtgctcaagg
     3481 tgtttggtac caatgctcac tttgtgaaga ggccttatga tgctgaagtc tccctaactg
     3541 ttcatggttt gctcctggtg gataccatgc agacatatgg tgctgatttt gaccttttga
     3601 tggcttcaca taagaacttg agctttgata ttccaacggg aagccttcgg gatagcaggg
     3661 cccagtctcc tgtctctgga ccgaatgtgg cccacttaac tgatggagct acactgaacg
     3721 accgatcagc tactagtgtt tcacttgaca aaattcttac caaagagcaa gagtccctta
     3781 ttaagttgga atatcagttt gtgagttcag agtgcccatc gatgaattta gacagtactc
     3841 ttcaggtgat ttccctacag gtgaataatt tagatattat cctcaatcca gagacgattg
     3901 tggagctaat tggttttctt caaaaatcct ttcccaagga aaaagatgat ttaagtcctc
     3961 aacctttaat gactgatttt gaaagaagct tcagagaaca aggaacttac cagtctacat
     4021 atgaacaaaa cactgaggtt gcagtggaaa tccataggct gaacttactg cttcttcgga
     4081 cagtgggcat ggcaaataga gagaaatatg gcagaaaaat tgcaactgca agtataggtg
     4141 gcaccaaagt taatgtctca atgggtagca cgtttgacat gaatggttct cttggctgtt
     4201 tacagcttat ggatttgaca caagataacg ttaaaaacca gtatgttgtc agcattggga
     4261 attctgtagg ctatgaaaat atcatcagtg atattggcta ctttgaatct gtgtttgtca
     4321 gaatggaaga tgcagccctc actgaagctt tgagtttcac gtttgttgag agatctaaac
     4381 aggagtgttt tctcaacctg aagatggctt ctttacatta taaccactct gctaagtttt
     4441 tgaaggagtt gacgttatcc atggatgaac tggaagaaaa ttttcgaggt atgctgaaaa
     4501 gcgcagccac caaagtcacc acagtactag ctaccaagac tgccgagtat agcgagatgg
     4561 tatcgctctt tgaaactcca aggaagactc gggaaccctt tatcttagag gaaaatgaaa
     4621 tatatgggtt tgacctagct tcgtctcatt tggacactgt aaagctaatc ttgaacataa
     4681 acattgaatc accagttgtt tctatccctc ggaagccggg gagtcctgag ttgttggtgg
     4741 gacacttggg acagatattc atccagaatt ttgtggcggg agatgatgaa tccagaagtg
     4801 accgtctgca ggtggaaatc aaggacatta aactgtattc tttgaattgc acccagttgg
     4861 caggtagaga agctgttggg tctgaaggaa gccggatgtt ttgcccacct tccgggtctg
     4921 gcagtgccaa cagtcaggag gaagctcatt tcacacgaca tgatttcttt gaatctttgc
     4981 atagaggtca agcttttcac atcctgaaca acaccaccat tcagtttaaa ctggagaaga
     5041 tccctataga gagagaatct gaattgactt tttctcttag cccagatgac ctgggaactt
     5101 ctagcatcat gaagattgaa ggaaaatttg tcaatccagt tcaggtggtg ttagcaaagc
     5161 atgtatatga gcaggtttta caaaccctgg acaatctcgt gtacagtgaa gatctgaata
     5221 agtatccagc cagtgctacc tcctcccctt gccctgattc tcctctgcct cccctcagta
     5281 cctgtggaga atcttctgtt gaaaggaagg agaatggatt gttcagccac tccagccttt
     5341 ctaacacctc tcagaagtca ttgtcagtga aggaagtcaa atcctttact cagattcaag
     5401 ccaccttttg tatatcagag cttcaggttc agctaagtgg agatctgact ttgggggccc
     5461 aaggtcttgt gagcttaaag tttcaggact ttgaggtgga attcagtaaa gaccatcccc
     5521 agactttatc tattcagatt gccctgcatt ctctgctgat ggaggactta ttggagaaga
     5581 atccagattc taaatataag aacctgatgg tgtctcgagg agcccctaag ccatctagtt
     5641 tagcacaaaa agaatacctt tctcagtctt gcccctcagt gtccaatgtg gaatatcctg
     5701 atatgcctcg gtctctccct tcccacatgg aagaagctcc taatgtcttc cagttgtatc
     5761 aaaggcccac ctctgcgtcc cggaaaaagc aaaaggaagt ccaagacaag gactatccct
     5821 tgaccccacc tccttctcca acagtggatg agcccaagat acttgttgga aagagtaaat
     5881 ttgatgattc cttagtccac atcaacatat tcttggtaga taagaaacat ccagaattct
     5941 cttccagtta caatcgagtt aaccggagca ttgatgttga ttttaattgc ttggatgtgc
     6001 tgatcacact gcaaacctgg gttgtgatat tagacttttt tggaatcggc tccactgcag
     6061 acaaccacgc aatgaggctg cctcctgagg gcattctgca caacgtgaag ttggagccac
     6121 atgcctccat ggagtctgga cttcaggatc cagtgaacac caaactggat ctcaaggttc
     6181 attcactttc tctagtgctg aataagacca ccagtgagct tgccaaagca aatgtgtcca
     6241 aattagtagc acacctggaa atgattgagg gagacctggc cttacagggc agcattggga
     6301 gtctgtctct aagtgacctc acatgccatg gagagttcta cagagaacgg ttcactacca
     6361 gtggtgaaga agcactcatc ttccagactt ttaaatacgg acggcctgac cctctgctcc
     6421 ggagagaaca cgacattcgc gtgagcctcc ggatggcctc tgtgcagtat gtgcatactc
     6481 agcgtttcca ggcagaggtg gtggccttca ttcagcattt cactcagctg caggatgtct
     6541 tagggcgcca gcgagctgct attgaggggc agacggtgag agatcaagcc cagcgctgtt
     6601 cacgggttct cctggatatt gaggctggtg ctcccgttct cttgatccca gaaagttcca
     6661 gatcaaataa tctgattgta gcaaatttgg ggaagttgaa agtcaaaaat aagtttctgt
     6721 ttgctggttt tcctggcacc ttttccctac aagataagga atctgtgcct tcagcttccc
     6781 caacgggtat tcccaaacac agtctgagga aaacgacaag cacggaggag cccaggggaa
     6841 cccattccca ggggcagttc acgatgcctc ttgctggaat gagcctagga agcctgaaga
     6901 gtgagtttgt gcccagtacc tccaccaagc agcaagggcc gcaacccaca ctgtctgttg
     6961 gccaagagtc cagtagtcca gaagaccatg tctgcctgct ggattgcgtt gtcgtggatc
     7021 tccaggacat ggacatcttt gctgcagaga gacatccgag agaatactcg aaggcaccag
     7081 aggatagtag tggagatctg atcttccctt cctattttgt gcgacagaca ggaggaagcc
     7141 tcttaaccga gccttgtagg ctgaaattgc aggtggaaag gaatttggac aaagaaataa
     7201 gtcatactgt gccagacata tctatccatg gcaatctctc ctcagtccac tgctctctgg
     7261 atctgtataa atacaagctg atccgcggct tattagagaa caacctggga gaacccatag
     7321 aggaatttat gcggccttat gatttacaag atccaagaat tcatactgtc ctgagtggag
     7381 aagtgtacac ctgtatgtgc ttcctcattg atatggtgaa tgtaagtctg gagcttaaag
     7441 atccaaaaag aaaagaaggt gctgggtccc tagccagatt tgacttcaag aaatgcaaac
     7501 tgctctatga aagtttttcc aaccaaacca agtccattaa cttggtttcc cattccatga
     7561 tggcttttga cacccgttat gctgggcaga agaccagccc tggcatgacg aatgtgttca
     7621 gctgtatctt tcagcccgct aagaacagca gcaccaccca agggtccatt cagattgaac
     7681 tacatttcag atctaccaag gattcctcct gctttacagt agttctcaac aatctccgtg
     7741 tgtttctcat atttgactgg ctactgttag tccatgattt tctccacact cccagtgata
     7801 ttaagaaaca aaatcatgtt actccttctc gccaccgtaa ctctagcagc gaatctgcta
     7861 tagttcccaa aactgtgaag agtggagtag ttaccaagcg gtcttccctt cctgtgtcca
     7921 atgaaaggca cctggaggtc aaggtcaatg taacaggtac ggagtttgtg gtcattgaag
     7981 atgtgtcctg cttcgacacc aatgccatta ttctgaaagg caccacagtg ctcacctata
     8041 agccccggtt tgttgatcgc cccttttcag gaagtttgtt tggcattgag gtgttttcat
     8101 gccgactagg gaatgagcat gatacagctc tttcaattgt ggatcccgta caaattcaaa
     8161 tggagttggt ggggaattct tcttatcaaa atagttcagg attgatggat gcattcaata
     8221 gtgaagattt cccacctgtc ctggagattc agttacaagc cctggatatc agactctcct
     8281 ataatgatgt tcagctgttt cttgccattg caaaatccat cccagagcaa gctaatgctg
     8341 cagtgccaga ctcagtggcc ctggagtcag actccgttgg cacttacctt ccaggtgcat
     8401 ctcgcgttgg agaggaaatc agagaaggga caagacacac cttagatcct gtcttggagt
     8461 tacagctggc taggctgcag gagctgggat tcagcatgga tgattgtcgc aaagctcttt
     8521 tggcgtgtca aggccaattg aaaaaggcag caagttggtt gtttaagaat gcggaacctc
     8581 tgaagtctct ttccttggcc tccaccagcc gagatagccc aggggctgtg gcagcgccat
     8641 tgatctctgg cgtggagatc aaagctgaga gtgtgtgcat ctgtttcatc gatgactgca
     8701 tggattgtga tgttcctctc gctgaactca ccttttcccg tctgaatttt cttcagcgtg
     8761 taagaactag ccctgaaggc tatgcccact tcaccctttc tggagattat tataaccgtg
     8821 ctctttcagg ctgggagcca tttattgagc cttggccatg ctctgtatcc tggcaacagc
     8881 aggcagctag tcgtctccat cctcctcgac tgaagctaga agccaaggcc aaacctcgtt
     8941 tggatatcaa tatcacttct gtgctaattg accagtatgt aagtaccaag gaatcgtgga
     9001 tggcagatta ctgtaaagat gacaaggaca tagagtcagc taaatcagaa gactggatgg
     9061 gctcttcggt ggatcctcca tgttttggac aatctagaat ggtgagcaag ggcgaggagc
     9121 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     9181 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     9241 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     9301 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     9361 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     9421 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     9481 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     9541 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     9601 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     9661 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc
     9721 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     9781 ccgggatcac tctcggcatg gacgagctgt acaagaccgg tgcagaggtg aaaaccccca
     9841 agcgccggca gccatttgtc ccctttgctc tgaggaacca cacggggtgc actttgtggt
     9901 ttgccaccct gaccaccaca cccaccagag ctgcactctc tcacagtggg agtccagggg
     9961 tagttccaga agggaacgga acatttctcg atgatactca caatgttagt gaatggcgag
    10021 aagtccttac aggtgaagag attccctttg aatttgaagc aagaggaaag ttaagacaca
    10081 gacacaccca tgacctccgg attcatcaac tgcaagtgag agtaaatggc tgggagcaag
    10141 tgagcccagt gtctgtggac aaagtcggga ccttttttcg atatgcagca ccagataaaa
    10201 attcatcttc ctctacgatt ggcagcccaa gcagcagaac aaatattata catccccagg
    10261 tttatttctc ttcactccca ccagtgcggg tggtctttgc agtgactatg gaaggcagtg
    10321 cacggaaagt catcactgtc cggtcagccc tcattgtgag gaacagactt gagacaccaa
    10381 tggaactaag actggatagc ccatcagctc cagacaagcc agtggtgctt cctgctatca
    10441 tgccagggga ttcgtttgct gtgcctttac acctcacttc ttggcggcta caggcccggc
    10501 ccaaaggatt gggtgtattt ttctgtaagg ctcccattca ttggaccaat gtagtgaaga
    10561 ctgcagaaat tagtagcagt aaacgagagt gccactctat ggacacagaa aaaagccgat
    10621 ttttcaggtt ttgtgtggct ataaagaaag agaattatcc agattatatg ccctcaaaca
    10681 tattttctga cagtgcaaaa cagattttca gacagcctgg gcataccata tatctcctgc
    10741 caactgtggt aatctgcaac ttgctaccct gtgaacttga tttttatgtt aaaggaatgc
    10801 caattaatgg gacgctgaaa cctggcaagg aggcagctct ccatacagct gatacatccc
    10861 agaacattga gctgggggta tcactggaga atttccccct ctgtaaagaa ttgctcattc
    10921 cacctggaac ccaaaactat atggtgagaa tgcgactcta tgacgtcaac cgtcggcagc
    10981 tgaacctcac catccggatt gtgtgtcgag cagaaggatc cttaaagatc ttcatttctg
    11041 ctccatattg gctgattaac aaaacagggt tgccactgat cttcagacag gacaatgcca
    11101 agacagatgc tgcaggccag tttgaggagc atgagctggc ccgtagcctg agtcctctct
    11161 tattctgcta tgctgacaaa gagcagccaa acctctgcac gatgagaatc ggaaggggga
    11221 ttcatccaga aggcatgccg ggctggtgtc agggcttctc cctggatggt ggtagtggtg
    11281 tccgagcttt gaaagtcatc cagcaaggaa accgcccagg gctgatctat aacattggta
    11341 ttgatgtcaa gaaaggccga ggtcgataca ttgatacctg catggtcatc tttgcccccc
    11401 gttacctgtt agataataaa tcatctcaca agcttgcatt tgcacagagg gaatttgcca
    11461 ggggacaggg aacagccaat cccgaaggtt acatttccac ccttcctggt tccagtgtgg
    11521 tgttccactg gcctcggaat gactatgatc agctattgtg tgtcagactg atggacgttc
    11581 ccaattgtat ttggtctgga ggctttgaag tcaacaagaa taattccttc catatcaaca
    11641 tgagggatac cttgggaaaa tgcttcttcc tacgagtgga aattactctc cgaggagcta
    11701 cgtataggat ctcatttagt gacacagatc agttacctcc tcctttccga attgacaact
    11761 tttctaaggt cccggttgtc tttactcagc atggcgtagc tgaacccagg ctccggactg
    11821 aagtgaagcc catgacttca ttggattatg cctgggacga acccaccttg ccacctttta
    11881 tcactctgac tgttaaaggg gcagggtcct ctgagatcaa ctgcaacatg aatgatttcc
    11941 aggataatcg gcagctttat tatgaaaatt tcatttacat tgctgctaca tatacattct
    12001 ctggcttgca ggagggaaca ggcaggcctg tggcttccaa caaggccatt acctgtgcgg
    12061 agctcgtttt ggatgtctca cccaagacac aaagagtcat tttaaaaaag aaggaaccag
    12121 gaaagcgttc tcagctgtgg aggatgacag gaacaggaat gctggcccat gagggctcct
    12181 cagttcctca caatcccaat aagccctcag ccgcccgctc caccgagggg tctgccatct
    12241 tagatattgc tggtctcgct gcagtgactg acaacagata cgagccactg atgctgagaa
    12301 agcctgaccg caggcgaagc acaactcaga cgtggagttt ccgagaagga aaactgacct
    12361 gtgggttaca tgggttggtc gtccaggcca aaggaggact ttctggtttg tttgatggag
    12421 ctgaagttgt tcttggtcct gacacttcca tggagctttt ggggccagtt ccacctgaac
    12481 aacaatttat taatcaaaaa atgagacctg gttctggaat gttatccatc agagtcatcc
    12541 cagatggacc aactagagca ctccagataa cagatttctg ccaccggaaa agcagccgtt
    12601 catatgaagt ggatgaactt cctgtcaccg aacaagagct gcagaaatta aagaatccag
    12661 atacagagca ggaattggaa gtgcttgtga ggttagaagg tggaattggg ttgtccttaa
    12721 ttaataaagt cccagaagaa ctggtctttg caagtcttac aggaatcaat gtgcactata
    12781 cacagctggc aaccagtcac atgcttgaac tcagcataca ggatgtacag gtggacaatc
    12841 agctcattgg taccacgcag cccttcatgc tctatgtgac tcccctgagc aatgagaatg
    12901 aggtcatcga gaccggccca gctgtgcaag tcaacgcagt gaagttcccc agtaagagtg
    12961 cactgaccaa catctacaag catctgatga tcacagctca gagattcaca gtgcaaattg
    13021 aggagaaact gctcctcaag ctgctaagtt tctttggcta cgatcaagca gaatcagagg
    13081 tggaaaaata tgatgaaaac ctccatgaaa agacagctga gcaaggtgga acaccaattc
    13141 gatactactt tgaaaatctc aaaatcagca ttcctcagat caagctaagt gtgttcacct
    13201 ccaacaagct cccattggat cttaaggccc taaaaagcac cttggggttt cctttgatac
    13261 ggtttgaaga cgctgtgatt aatctagatc cattcactcg ggtacatccc tatgagacca
    13321 aggagttcat catcaatgat atcctcaaac atttccagga ggaactcctc agccaggcag
    13381 ctcgaatcct gggatcagtg gattttcttg gcaatcctat ggggcttttg aatgatgttt
    13441 ctgaaggggt tactggactg ataaaatatg gaaatgtcgg gggcctcatc agaaatgtta
    13501 cacacggagt atcaaactct gctgccaagt ttgctggaac attatcagat ggcttaggga
    13561 agacgatgga caatcggcat cagtcagagc gggagtacat caggtaccat gcagccacaa
    13621 gtggtgaaca ccttgtagcc ggcatccatg gcctggctca tggtatcatt ggtggactga
    13681 ccagtgttat aacttcgaca gtggaaggtg tgaaaacaga agggggtgtc agcggtttca
    13741 tatctggcct tggaaaaggg cttgttggca ctgtaaccaa gccagtggca ggcgccctgg
    13801 attttgcatc agaaacagcc caggcggtga gagacacagc cacactcagc ggccccagga
    13861 ctcaagcaca gagggttcgg aaaccgcgtt gctgcacggg gccccagggg ctgcttcccc
    13921 gatattctga gagccaggcg gaaggacagg agcagctctt caaactcaca gacaacatac
    13981 aggacgaatt cttcatcgct gtggagaaca ttgacagcta ctgcgtgctc atctcctcca
    14041 aagctgttta cttcctgaaa agtggagact acgtggatcg agaagccatt ttcctagaag
    14101 tcaaatacga tgacctctac cactgccttg tctccaaaga ccatgggaag gtgtatgtgc
    14161 aggtgaccaa gaaagccgtg agcacgagca gtggagtgtc catccccggc ccctcccacc
    14221 agaagcccat ggtccatgtg aaatctgagg tccttgctgt caagttgtca caagaaataa
    14281 actacgcaaa gagcctctac tatgaacagc agcttatgtt aagactcagc gaaaaccgag
    14341 agcagctgga gctggactcc tgagtcgacg gtaccgcggg cccgggatcc accggatcta
    14401 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    14461 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    14521 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    14581 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
    14641 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    14701 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    14761 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    14821 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    14881 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    14941 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    15001 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    15061 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
    15121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    15181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa
    15241 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
    15301 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
    15361 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
    15421 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
    15481 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
    15541 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
    15601 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
    15661 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
    15721 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
    15781 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
    15841 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
    15901 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
    15961 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
    16021 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
    16081 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
    16141 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
    16201 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
    16261 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
    16321 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
    16381 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
    16441 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
    16501 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
    16561 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
    16621 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
    16681 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
    16741 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
    16801 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
    16861 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    16921 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    16981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    17041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    17101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    17161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    17221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    17281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    17341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    17401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    17461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    17521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    17581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    17641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    17701 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
    17761 gttcatagcc catatatgga gttccg
//
