LOCUS       sequence-346242-       12049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For lentiviral transduction of 3FLAG-EEA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12049)
  AUTHORS   Park H, Hundley FV, Yu Q, Overmyer KA, Brademan DR, Serrano L, Paulo
            JA, Paoli JC, Swarup S, Coon JJ, Gygi SP, Wade Harper J
  TITLE     Spatial snapshots of amyloid precursor protein intramembrane 
            processing via early endosome proteomics.
  JOURNAL   Nat Commun. 2022 Oct 16;13(1):6112. doi: 10.1038/s41467-022-33881-x.
  PUBMED    36245040
REFERENCE   2  (bases 1 to 12049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-10-16"; volume: "13(1):6112. doi"; pages: " 
            10.1038/s41467-022-33881-x"
COMMENT     Sequence Label: pHAGE-3FLAG-EEA1
FEATURES             Location/Qualifiers
     source          1..12049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    218..239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(254..259,260..277,278..284)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    292..308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     297..319
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     316..332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     316..332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     382..401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             593..1226
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1273..1398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1895..2128
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2313..2357
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2654..2771
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2823..3126
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3127..3330
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3281..3301
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3327..3351
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3427..3436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3436..3459
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3463..3486
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3490..3513
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    3529..3553
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3559..7794
                     /codon_start=1
                     /product="human early endosome antigen 1"
                     /label=EEA1
                     /translation="MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQ
                     CMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKW
                     YSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQ
                     KAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIED
                     VAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQ
                     EVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLH
                     QKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQ
                     QQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADL
                     QLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQ
                     IGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQ
                     VTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKE
                     KVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVT
                     AKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDL
                     QQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKIL
                     KQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKN
                     SKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLK
                     LELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQK
                     TELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQD
                     LKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQD
                     IQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQ
                     LLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEK
                     EVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEAT
                     VQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQ
                     ALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVC
                     DACFNDLQG"
     protein_bind    complement(7796..7820)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7833..8401
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7851..8403
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7995..8012)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8222..8241
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8415..9014
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8415..8434)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8911..8931
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9031..9619
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9084..9104)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9502..9513)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9694..9927
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10046..10064)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10132..10236
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10237..10305,10306..11097)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10455..10474)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11268..11856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11757..11776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12010..12027
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact
       61 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
      121 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt
      181 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      241 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      301 gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat
      361 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      421 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
      481 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
      541 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
      601 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
      661 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
      721 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
      781 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
      841 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
      901 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
      961 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     1021 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     1081 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     1141 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     1201 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     1261 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     1321 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     1381 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     1441 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     1501 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     1561 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     1621 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     1681 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     1741 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1801 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1861 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1921 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1981 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     2041 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     2101 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     2161 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     2221 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     2281 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     2341 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     2401 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     2461 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2521 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2581 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattaattca caaatggcag
     2641 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2701 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2761 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact agtggagttc
     2821 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2881 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2941 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     3001 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     3061 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     3121 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     3181 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     3241 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     3301 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     3361 cgccatccac gctgttttga cctccataga agacaccggc ggccgcagat ctctcgaggt
     3421 tgatctgcca ccatggacta caaggatgac gatgacaagg gcgattacaa ggatgacgac
     3481 gataagggag attacaagga tgacgacgat aaggctcgag gttatcaaac aagtttgtac
     3541 aaaaaagcag gctccaccat gttaaggagg attttacaga ggactcctgg gagagttggc
     3601 tctcaaggtt ctgatttaga ttcatcagca actcctataa acacagtgga cgtcaataat
     3661 gaaagctctt cagagggttt catatgtccc cagtgtatga aatctcttgg atctgctgat
     3721 gaacttttca aacattatga agctgttcat gatgctggta atgactcagg tcatggagga
     3781 gagtctaatc ttgctttgaa gcgagatgat gtaacactgc tcagacaaga ggtccaagac
     3841 ctacaggctt cacttaagga agaaaaatgg tactcggaag aattaaagaa ggaattagaa
     3901 aaatatcaag ggctgcagca gcaagaggcc aaacctgatg ggttggtgac tgattcatca
     3961 gcagaactac agtctttgga acagcaatta gaagaagccc aaacagaaaa ttttaatatt
     4021 aagcaaatga aagacttatt tgaacagaaa gcagcccaac ttgctactga aattgcagat
     4081 ataaagtcaa agtatgatga agaaaggagt cttcgagaag ctgctgaaca aaaagtgaca
     4141 cgtctgacag aagaattaaa caaagaggca actgtaattc aagatctgaa gacggaactg
     4201 cttcagagac ctggtataga agatgttgcc gtgctaaaga aagaactggt ccaagttcaa
     4261 acactaatgg ataacatgac cttggaacgt gagcgagaat ctgaaaaact caaagatgaa
     4321 tgcaaaaaat tgcagtcaca atatgctagc tcagaggcca caataagcca gctaaggagt
     4381 gaacttgcca aaggccccca ggaagttgct gtatatgtac aggaactaca aaaactgaaa
     4441 agttcagtta atgaattaac acaaaaaaat cagaccttga cagaaaactt gctgaaaaaa
     4501 gaacaagact atactaagtt agaggagaaa cataatgaag aatctgtgag taaaaagaat
     4561 attcaggcaa cccttcatca aaaagaccta gattgtcaac agcttcagtc aagattgtct
     4621 gcatctgaaa cctcactgca tagaatacat gtagaactaa gtgaaaaagg agaagctact
     4681 caaaagctca aagaagaatt atctgaggta gagaccaagt accagcatct aaaggcggag
     4741 tttaagcagc tacaacaaca gagagaagaa aaggagcagc atgggttaca actccaaagt
     4801 gaaattaatc aattacatag caaacttctg gagacagagc gccaactagg ggaagctcat
     4861 ggtaggctga aggaacagag acagctttca agtgaaaagt tgatggataa agaacaacaa
     4921 gtggctgatt tacaactcaa actttctcgg ttagaagagc agttgaagga aaaagttaca
     4981 aattctacag aattgcagca tcaattagat aaaacaaagc aacagcatca agaacaacag
     5041 gctcttcagc aaagcaccac ggcaaaactt cgagaagctc agaatgattt ggaacaagtt
     5101 ctacgtcaaa ttggcgataa ggaccaaaag atccagaacc ttgaagcttt attacagaag
     5161 agtaaagaaa atatttcatt actagaaaaa gaaagagaag atctttatgc aaaaattcag
     5221 gctggtgaag gagagactgc tgttcttaac cagttacaag aaaaaaacca tacactacag
     5281 gagcaagtaa ctcaactaac agagaagctg aagaatcagt cagaaagtca taaacaagcc
     5341 caggagaatt tgcatgacca ggtacaagag cagaaggcac atcttagagc tgcacaagac
     5401 cgtgtccttt ccctagaaac tagtgtcaat gaattaaata gtcaattaaa tgaaagcaag
     5461 gagaaggtct cccagcttga catacagatt aaagccaaaa ccgaactatt actatcagca
     5521 gaagcagcaa aaactgctca aagagctgat cttcagaatc atttggacac agctcaaaat
     5581 gcattacaag ataaacagca ggagttaaat aagattacta ctcagttgga tcaggtcact
     5641 gcaaagttac aagacaagca agaacattgc agtcagctgg aaagtcatct taaagaatat
     5701 aaagagaaat acctctcttt agaacagaaa accgaagagc tagaaggtca aattaagaaa
     5761 ctagaagctg atagtcttga agttaaagca agcaaggagc aggctttgca agatctacaa
     5821 cagcaaagac agctgaacac agatttagag ctcagagcca cagaattgag taaacaactt
     5881 gaaatggaga aggaaatagt atccagtaca agattggatc tacagaaaaa atctgaagcc
     5941 cttgaaagta tcaagcaaaa gcttaccaag caagaggaag aaaaaaaaat cctgaaacaa
     6001 gattttgaaa ctttaagtca agaaacaaag attcagcatg aggaattgaa taacagaatt
     6061 caaacaacag taacagaact acaaaaagtg aaaatggaga aagaagcttt aatgacagag
     6121 ctttctacag taaaggacaa actatcaaaa gtttctgatt ctttgaaaaa ctctaaaagt
     6181 gaatttgaaa aggagaatca gaaaggaaaa gccgctatat tagacttgga aaaaacttgc
     6241 aaagaattaa agcatcaact tcaagtgcag atggaaaaca cacttaagga acagaaggaa
     6301 ctgaaaaagt cacttgaaaa agagaaggag gcttctcatc agttgaaatt ggaactcaat
     6361 tcaatgcagg aacaacttat acaggcccag aatactttaa aacaaaatga aaaagaagag
     6421 caacaacttc aggggaacat aaatgagcta aagcaatcaa gtgaacagaa gaaaaaacaa
     6481 attgaagcac tccaaggaga gcttaaaatt gctgttttac agaagacaga gcttgagaat
     6541 aaactacagc agcagttaac acaggcagcc caggaacttg cagcagagaa agagaaaata
     6601 tcagtattac aaaacaacta tgaaaaaagt caggaaactt tcaaacagct tcaatctgat
     6661 ttctatggga gggaatctga acttctagcc accaggcaag atcttaagtc tgtagaagag
     6721 aagctttctc tagcacagga ggacttgatt tcaaacagaa atcaaattgg aaatcaaaat
     6781 aaattgattc aagaactgaa gactgccaag gctacattgg agcaggattc agcaaagaaa
     6841 gaacagcaat tgcaggagcg atgtaaagca ctacaagaca ttcagaaaga aaagtcactg
     6901 aaagaaaaag aactggtaaa tgagaagtct aaattggcag agatagaaga aattaaatgt
     6961 agacaagaaa aagaaatcac taaactaaac gaagaactca agtcccacaa actagaaagc
     7021 ataaaggaga taacaaatct taaagatgct aaacagcttc taattcagca gaaattagaa
     7081 cttcaaggaa aagcggactc cctgaaggca gctgttgaac aggagaagag aaatcagcag
     7141 atactaaaag accaggtgaa aaaggaagaa gaggagctga agaaagaatt tattgagaaa
     7201 gaagctaagt tgcattccga aataaaagaa aaggaagtag gaatgaagaa gcatgaagaa
     7261 aatgaggcta aacttaccat gcagattaca gcattaaatg aaaacttagg cactgtgaag
     7321 aaggagtggc aatctagtca acggagagtt agtgagcttg agaaacaaac ggatgactta
     7381 cggggtgaaa ttgcagtatt agaagcaacg gttcagaata atcaagatga aaggagagca
     7441 ctactggaaa gatgtcttaa aggagaaggt gaaatagaaa agcttcaaac caaagtatta
     7501 gaattgcaaa gaaagctgga taatacaact gcagcagtgc aggagctggg cagagaaaac
     7561 caatcacttc agatcaaaca tacacaagcg ttgaatagaa agtgggccga agacaatgaa
     7621 gtacaaaact gtatggcctg tgggaaaggc ttttcagtaa cagtgagacg gcatcactgc
     7681 cgacagtgtg gaaatatctt ctgtgctgaa tgttcagcca aaaatgcctt aactccttcc
     7741 tccaagaagc ctgttcgtgt ctgtgatgca tgtttcaatg acttgcaagg ataagaccca
     7801 gctttcttgt acaaagtggt tcgataacga attccgcccc ccccccccta acgttactgg
     7861 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     7921 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7981 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     8041 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     8101 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     8161 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     8221 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     8281 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     8341 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     8401 atatggccac aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     8461 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     8521 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     8581 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     8641 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     8701 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     8761 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     8821 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     8881 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     8941 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     9001 agcccggtgc ctgatgcatc gatggatcct aatcaacctc tggattacaa aatttgtgaa
     9061 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9121 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9181 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9241 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9301 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9361 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9421 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9481 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9541 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     9601 ctttgggccg cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag
     9661 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9721 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9781 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9841 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9901 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     9961 tataacttgc aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg
    10021 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
    10081 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    10141 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    10201 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    10261 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10321 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10381 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10441 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    10501 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10561 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10621 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10681 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10741 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10801 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10861 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10921 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10981 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    11041 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    11101 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    11161 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    11221 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    11281 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    11341 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    11401 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    11461 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    11521 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    11581 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    11641 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    11701 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    11761 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    11821 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    11881 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    11941 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    12001 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatac
//
