LOCUS       sequence-346244-       16794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express VPS13D lacking C-terminal 407 aa in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Baldwin HA, Wang C, Kanfer G, Shah HV, Velayos-Baeza A, 
            Dulovic-Mahlow M, Bruggemann N, Anding A, Baehrecke EH, Maric D, 
            Prinz WA, Youle RJ
  TITLE     VPS13D promotes peroxisome biogenesis.
  JOURNAL   J Cell Biol. 2021 May 3;220(5). pii: 212018. doi: 
            10.1083/jcb.202001188.
  PUBMED    33891012
REFERENCE   2  (bases 1 to 16794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-05-3"; volume: "220(5). pii: 212018. doi"; 
            pages: " 10.1083/jcb.202001188"
COMMENT     Sequence Label: pGFP-Ub-VPS13D_dC
FEATURES             Location/Qualifiers
     source          1..16794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1369..1593
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     polyA_signal    13582..13703
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13619..13638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13673..13692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(13710..14165)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13847..13868)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14059..14078
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        14192..14296
                     /gene="bla"
                     /label=AmpR promoter
     promoter        14298..14655
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        14298..14489
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(14321..14341)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      14506..14641
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             14690..15484
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             14690..15484
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14744..14763)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15354..15373
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(15672..15691)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    15716..15763
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      16092..16680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16581..16600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcgg gccgcctgca gatcttcgtg
     1381 aagactctga ctggtaagac catcaccctc gaggttgagc ccagtgacac cattgagaat
     1441 gtcaaggcaa agatccaaga taaggaaggc atccctcctg accagcagag gctgatcttt
     1501 gctggaaaac agctggaaga tgggcgcacc ctgtctgact acaacatcca gaaagagtcc
     1561 accctgcacc tggtactccg tctcagaggt gggatgttgg aaggccttgt agcctgggtt
     1621 ctcaatacct atttgggaaa atatgtcaat aacctgaaca ctgaccagct ctcagttgca
     1681 cttctcaaag gtgctgttga attagaaaac ttgccattaa agaaagatgc cttgaaagaa
     1741 ttggaattac catttgaagt caaagctggc ttcattggga aagtaaccct tcagattccc
     1801 ttttatcgcc cccatgtgga cccttgggtg atctccatct ccagccttca cttaattgga
     1861 gccccagaga aaatacagga tttcaatgat gaaaaggaga agctgttgga aagggaacgt
     1921 aagaaagcac tacttcaagc cctggaggag aaatggaaga atgaccgcca gcagaaaggg
     1981 gagtcctatt ggtattcagt taccgcctcc gtagttacaa ggattgtgga gaatattgaa
     2041 ttaaaaattc aagatgtcca tttacgcttt gaagatggtg tcaccaatcc ctcccatcct
     2101 tttgcttttg gcatctgcat taagaatgtg tccatgcaaa atgctgtgaa tgagcctgta
     2161 cagaaactaa tgcggaaaaa gcaattagac gtagcagaat ttagcatcta ttgggatgtc
     2221 gattgcactt tactggggga tttgcctcag atggagttac aggaggccat ggccaggagc
     2281 atggagagtc gcagccatca ctacgtcctg gagcctgtgt ttgcatctgc tcttttgaag
     2341 agaaactgct ccaagaagcc cctgcggtct cggcacagtc cccgtattga ttgtgatatt
     2401 cagctggaga ccattccctt gaaactctct cagctgcaat accggcaaat catggaattc
     2461 ctcaaggagc tggaacgaaa ggagaggcag gtgaagttcc gaaggtggaa acccaaggtg
     2521 gcgatatcta agaactgccg agaatggtgg tattttgctt tgaatgctaa cttgtatgag
     2581 atcagagagc agaggaaacg ttgcacctgg gactttatgt tgcaccgcgc tcgtgatgct
     2641 gtatcttaca ctgacaaata tttcaacaag ttaaaaggag gcctgctgtc cacagatgac
     2701 aaggaggaaa tgtgtcggat tgaagaggaa cagagctttg aggaattgaa gattttgcgt
     2761 gaactggttc atgatcgatt tcacaaacag gaagaactag cagagagtct gcgggagcct
     2821 cagtttgatt ctccaggagc ctgtccggga gccccagaac ccggtggagg cagtgggatg
     2881 ctgcagtatc tccagtcctg gtttcctgga tggggtggct ggtacgggca gcagacccca
     2941 gaagggaatg tggttgaggg actgtcagca gagcaacagg agcagtggat tcctgaagag
     3001 atcctgggca ctgaggagtt ttttgacccc actgcagatg cctcgtgtat gaacacgtat
     3061 acaaagcgag atcatgtctt tgccaaactg aatttgcagt tgcagcgagg tacagtgact
     3121 ctgttacaca aggagcaagg aactcctcaa atgaatgaaa gtgctttcat gcagctcgag
     3181 ttttcagatg taaaacttct agcagagtct cttcctcgaa gaaattcctc gttgctttca
     3241 gtccggttgg gtggactgtt tcttcgagac ctggctacag aaggaactat gtttcctctt
     3301 ctagtcttcc ctaatccaca aaaagaagtt ggcagagtct cacaatcttt tggtctacaa
     3361 actacatctg cagacagaag tgatcattac ccagctgcag atccagatgg ccccgttttt
     3421 gagatgctgt atgagagaaa tccggcgcac agccactttg agaggcggct caatgtcagc
     3481 acaaggccct tgaacatcat atacaatccg caggccatta aaaaagtagc agactttttc
     3541 tacaagggaa aggttcatac ctcaggtttt ggttatcagt ctgaacttga gctgagagtg
     3601 gctgaagctg cccgaagaca atataacaag ctgaagatgc agaccaaggc agaaatccgg
     3661 caaactcttg atcgtttgct agtgggtgat ttcattgagg agagtaaacg atggaccgtg
     3721 cggctggata tttctgcccc tcaggtgata tttcctgatg atttcaaatt caagaatcct
     3781 gtgttagttg tcgtggatct aggaagaatg cttttgacga acacccaaga taactccagg
     3841 aggaaaagta gggatgggtc agcatctgaa gagacccagt ttagtgatga tgaatataag
     3901 acccccctgg ccacacctcc taacacccca cctcccgagt caagcagcag caacggagag
     3961 aaaacacctc ccttttctgg agttgagttc agtgaagaac agcttcaagc acatttaatg
     4021 agcacaaaga tgtatgagag gtactcgctg tcatttatgg acctccagat catggttgga
     4081 cgagtgaaag acaattggaa gcatgtccag gatattgacg tgggaccaac acatgtggta
     4141 gagaagttca acgttcacct acagttagag cgtcgattga tttatacttc agatcccaaa
     4201 tatccaggag ccgtgctctc aggcaactta ccagacttaa aaatccacat taatgaagat
     4261 aaaatatctg cactaaagaa ttgctttgct ctcctcacca ccccagaaat gaaaacttct
     4321 gacactcaga ttaaagaaaa gatttttccc caggaggagc agcggggaag tttgcaagac
     4381 tccgtaatga atttaaccca gagcattgtg ttgttggagc agcatacccg cgaggttctg
     4441 gtggagtcgc agctcctcct ggcggaattt aaagtgaact gtatgcagct tggtgttgag
     4501 agcaatggcc ggtacatttc tgtgctcaag gtgtttggta ccaatgctca ctttgtgaag
     4561 aggccttatg atgctgaagt ctccctaact gttcatggtt tgctcctggt ggataccatg
     4621 cagacatatg gtgctgattt tgaccttttg atggcttcac ataagaactt gagctttgat
     4681 attccaacgg gaagccttcg ggatagcagg gcccagtctc ctgtctctgg accgaatgtg
     4741 gcccacttaa ctgatggagc tacactgaac gaccgatcag ctactagtgt ttcacttgac
     4801 aaaattctta ccaaagagca agagtccctt attaagttgg aatatcagtt tgtgagttca
     4861 gagtgcccat cgatgaattt agacagtact cttcaggtga tttccctaca ggtgaataat
     4921 ttagatatta tcctcaatcc agagacgatt gtggagctaa ttggttttct tcaaaaatcc
     4981 tttcccaagg aaaaagatga tttaagtcct caacctttaa tgactgattt tgaaagaagc
     5041 ttcagagaac aaggaactta ccagtctaca tatgaacaaa acactgaggt tgcagtggaa
     5101 atccataggc tgaacttact gcttcttcgg acagtgggca tggcaaatag agagaaatat
     5161 ggcagaaaaa ttgcaactgc aagtataggt ggcaccaaag ttaatgtctc aatgggtagc
     5221 acgtttgaca tgaatggttc tcttggctgt ttacagctta tggatttgac acaagataac
     5281 gttaaaaacc agtatgttgt cagcattggg aattctgtag gctatgaaaa tatcatcagt
     5341 gatattggct actttgaatc tgtgtttgtc agaatggaag atgcagccct cactgaagct
     5401 ttgagtttca cgtttgttga gagatctaaa caggagtgtt ttctcaacct gaagatggct
     5461 tctttacatt ataaccactc tgctaagttt ttgaaggagt tgacgttatc catggatgaa
     5521 ctggaagaaa attttcgagg tatgctgaaa agcgcagcca ccaaagtcac cacagtacta
     5581 gctaccaaga ctgccgagta tagcgagatg gtatcgctct ttgaaactcc aaggaagact
     5641 cgggaaccct ttatcttaga ggaaaatgaa atatatgggt ttgacctagc ttcgtctcat
     5701 ttggacactg taaagctaat cttgaacata aacattgaat caccagttgt ttctatccct
     5761 cggaagccgg ggagtcctga gttgttggtg ggacacttgg gacagatatt catccagaat
     5821 tttgtggcgg gagatgatga atccagaagt gaccgtctgc aggtggaaat caaggacatt
     5881 aaactgtatt ctttgaattg cacccagttg gcaggtagag aagctgttgg gtctgaagga
     5941 agccggatgt tttgcccacc ttccgggtct ggcagtgcca acagtcagga ggaagctcat
     6001 ttcacacgac atgatttctt tgaatctttg catagaggtc aagcttttca catcctgaac
     6061 aacaccacca ttcagtttaa actggagaag atccctatag agagagaatc tgaattgact
     6121 ttttctctta gcccagatga cctgggaact tctagcatca tgaagattga aggaaaattt
     6181 gtcaatccag ttcaggtggt gttagcaaag catgtatatg agcaggtttt acaaaccctg
     6241 gacaatctcg tgtacagtga agatctgaat aagtatccag ccagtgctac ctcctcccct
     6301 tgccctgatt ctcctctgcc tcccctcagt acctgtggag aatcttctgt tgaaaggaag
     6361 gagaatggat tgttcagcca ctccagcctt tctaacacct ctcagaagtc attgtcagtg
     6421 aaggaagtca aatcctttac tcagattcaa gccacctttt gtatatcaga gcttcaggtt
     6481 cagctaagtg gagatctgac tttgggggcc caaggtcttg tgagcttaaa gtttcaggac
     6541 tttgaggtgg aattcagtaa agaccatccc cagactttat ctattcagat tgccctgcat
     6601 tctctgctga tggaggactt attggagaag aatccagatt ctaaatataa gaacctgatg
     6661 gtgtctcgag gagcccctaa gccatctagt ttagcacaaa aagaatacct ttctcagtct
     6721 tgcccctcag tgtccaatgt ggaatatcct gatatgcctc ggtctctccc ttcccacatg
     6781 gaagaagctc ctaatgtctt ccagttgtat caaaggccca cctctgcgtc ccggaaaaag
     6841 caaaaggaag tccaagacaa ggactatccc ttgaccccac ctccttctcc aacagtggat
     6901 gagcccaaga tacttgttgg aaagagtaaa tttgatgatt ccttagtcca catcaacata
     6961 ttcttggtag ataagaaaca tccagaattc tcttccagtt acaatcgagt taaccggagc
     7021 attgatgttg attttaattg cttggatgtg ctgatcacac tgcaaacctg ggttgtgata
     7081 ttagactttt ttggaatcgg ctccactgca gacaaccacg caatgaggct gcctcctgag
     7141 ggcattctgc acaacgtgaa gttggagcca catgcctcca tggagtctgg acttcaggat
     7201 ccagtgaaca ccaaactgga tctcaaggtt cattcacttt ctctagtgct gaataagacc
     7261 accagtgagc ttgccaaagc aaatgtgtcc aaattagtag cacacctgga aatgattgag
     7321 ggagacctgg ccttacaggg cagcattggg agtctgtctc taagtgacct cacatgccat
     7381 ggagagttct acagagaacg gttcactacc agtggtgaag aagcactcat cttccagact
     7441 tttaaatacg gacggcctga ccctctgctc cggagagaac acgacattcg cgtgagcctc
     7501 cggatggcct ctgtgcagta tgtgcatact cagcgtttcc aggcagaggt ggtggccttc
     7561 attcagcatt tcactcagct gcaggatgtc ttagggcgcc agcgagctgc tattgagggg
     7621 cagacggtga gagatcaagc ccagcgctgt tcacgggttc tcctggatat tgaggctggt
     7681 gctcccgttc tcttgatccc agaaagttcc agatcaaata atctgattgt agcaaatttg
     7741 gggaagttga aagtcaaaaa taagtttctg tttgctggtt ttcctggcac cttttcccta
     7801 caagataagg aatctgtgcc ttcagcttcc ccaacgggta ttcccaaaca cagtctgagg
     7861 aaaacgacaa gcacggagga gcccagggga acccattccc aggggcagtt cacgatgcct
     7921 cttgctggaa tgagcctagg aagcctgaag agtgagtttg tgcccagtac ctccaccaag
     7981 cagcaagggc cgcaacccac actgtctgtt ggccaagagt ccagtagtcc agaagaccat
     8041 gtctgcctgc tggattgcgt tgtcgtggat ctccaggaca tggacatctt tgctgcagag
     8101 agacatccga gagaatactc gaaggcacca gaggatagta gtggagatct gatcttccct
     8161 tcctattttg tgcgacagac aggaggaagc ctcttaaccg agccttgtag gctgaaattg
     8221 caggtggaaa ggaatttgga caaagaaata agtcatactg tgccagacat atctatccat
     8281 ggcaatctct cctcagtcca ctgctctctg gatctgtata aatacaagct gatccgcggc
     8341 ttattagaga acaacctggg agaacccata gaggaattta tgcggcctta tgatttacaa
     8401 gatccaagaa ttcatactgt cctgagtgga gaagtgtaca cctgtatgtg cttcctcatt
     8461 gatatggtga atgtaagtct ggagcttaaa gatccaaaaa gaaaagaagg tgctgggtcc
     8521 ctagccagat ttgacttcaa gaaatgcaaa ctgctctatg aaagtttttc caaccaaacc
     8581 aagtccatta acttggtttc ccattccatg atggcttttg acacccgtta tgctgggcag
     8641 aagaccagcc ctggcatgac gaatgtgttc agctgtatct ttcagcccgc taagaacagc
     8701 agcaccaccc aagggtccat tcagattgaa ctacatttca gatctaccaa ggattcctcc
     8761 tgctttacag tagttctcaa caatctccgt gtgtttctca tatttgactg gctactgtta
     8821 gtccatgatt ttctccacac tcccagtgat attaagaaac aaaatcatgt tactccttct
     8881 cgccaccgta actctagcag cgaatctgct atagttccca aaactgtgaa gagtggagta
     8941 gttaccaagc ggtcttccct tcctgtgtcc aatgaaaggc acctggaggt caaggtcaat
     9001 gtaacaggta cggagtttgt ggtcattgaa gatgtgtcct gcttcgacac caatgccatt
     9061 attctgaaag gcaccacagt gctcacctat aagccccggt ttgttgatcg ccccttttca
     9121 ggaagtttgt ttggcattga ggtgttttca tgccgactag ggaatgagca tgatacagct
     9181 ctttcaattg tggatcccgt acaaattcaa atggagttgg tggggaattc ttcttatcaa
     9241 aatagttcag gattgatgga tgcattcaat agtgaagatt tcccacctgt cctggagatt
     9301 cagttacaag ccctggatat cagactctcc tataatgatg ttcagctgtt tcttgccatt
     9361 gcaaaatcca tcccagagca agctaatgct gcagtgccag actcagtggc cctggagtca
     9421 gactccgttg gcacttacct tccaggtgca tctcgcgttg gagaggaaat cagagaaggg
     9481 acaagacaca ccttagatcc tgtcttggag ttacagctgg ctaggctgca ggagctggga
     9541 ttcagcatgg atgattgtcg caaagctctt ttggcgtgtc aaggccaatt gaaaaaggca
     9601 gcaagttggt tgtttaagaa tgcggaacct ctgaagtctc tttccttggc ctccaccagc
     9661 cgagatagcc caggggctgt ggcagcgcca ttgatctctg gcgtggagat caaagctgag
     9721 agtgtgtgca tctgtttcat cgatgactgc atggattgtg atgttcctct cgctgaactc
     9781 accttttccc gtctgaattt tcttcagcgt gtaagaacta gccctgaagg ctatgcccac
     9841 ttcacccttt ctggagatta ttataaccgt gctctttcag gctgggagcc atttattgag
     9901 ccttggccat gctctgtatc ctggcaacag caggcagcta gtcgtctcca tcctcctcga
     9961 ctgaagctag aagccaaggc caaacctcgt ttggatatca atatcacttc tgtgctaatt
    10021 gaccagtatg taagtaccaa ggaatcgtgg atggcagatt actgtaaaga tgacaaggac
    10081 atagagtcag ctaaatcaga agactggatg ggctcttcgg tggatcctcc atgttttgga
    10141 caaacagagg tgaaaacccc caagcgccgg cagccatttg tcccctttgc tctgaggaac
    10201 cacacggggt gcactttgtg gtttgccacc ctgaccacca cacccaccag agctgcactc
    10261 tctcacagtg ggagtccagg ggtagttcca gaagggaacg gaacatttct cgatgatact
    10321 cacaatgtta gtgaatggcg agaagtcctt acaggtgaag agattccctt tgaatttgaa
    10381 gcaagaggaa agttaagaca cagacacacc catgacctcc ggattcatca actgcaagtg
    10441 agagtaaatg gctgggagca agtgagccca gtgtctgtgg acaaagtcgg gacctttttt
    10501 cgatatgcag caccagataa aaattcatct tcctctacga ttggcagccc aagcagcaga
    10561 acaaatatta tacatcccca ggtttatttc tcttcactcc caccagtgcg ggtggtcttt
    10621 gcagtgacta tggaaggcag tgcacggaaa gtcatcactg tccggtcagc cctcattgtg
    10681 aggaacagac ttgagacacc aatggaacta agactggata gcccatcagc tccagacaag
    10741 ccagtggtgc ttcctgctat catgccaggg gattcgtttg ctgtgccttt acacctcact
    10801 tcttggcggc tacaggcccg gcccaaagga ttgggtgtat ttttctgtaa ggctcccatt
    10861 cattggacca atgtagtgaa gactgcagaa attagtagca gtaaacgaga gtgccactct
    10921 atggacacag aaaaaagccg atttttcagg ttttgtgtgg ctataaagaa agagaattat
    10981 ccagattata tgccctcaaa catattttct gacagtgcaa aacagatttt cagacagcct
    11041 gggcatacca tatatctcct gccaactgtg gtaatctgca acttgctacc ctgtgaactt
    11101 gatttttatg ttaaaggaat gccaattaat gggacgctga aacctggcaa ggaggcagct
    11161 ctccatacag ctgatacatc ccagaacatt gagctggggg tatcactgga gaatttcccc
    11221 ctctgtaaag aattgctcat tccacctgga acccaaaact atatggtgag aatgcgactc
    11281 tatgacgtca accgtcggca gctgaacctc accatccgga ttgtgtgtcg agcagaagga
    11341 tccttaaaga tcttcatttc tgctccatat tggctgatta acaaaacagg gttgccactg
    11401 atcttcagac aggacaatgc caagacagat gctgcaggcc agtttgagga gcatgagctg
    11461 gcccgtagcc tgagtcctct cttattctgc tatgctgaca aagagcagcc aaacctctgc
    11521 acgatgagaa tcggaagggg gattcatcca gaaggcatgc cgggctggtg tcagggcttc
    11581 tccctggatg gtggtagtgg tgtccgagct ttgaaagtca tccagcaagg aaaccgccca
    11641 gggctgatct ataacattgg tattgatgtc aagaaaggcc gaggtcgata cattgatacc
    11701 tgcatggtca tctttgcccc ccgttacctg ttagataata aatcatctca caagcttgca
    11761 tttgcacaga gggaatttgc caggggacag ggaacagcca atcccgaagg ttacatttcc
    11821 acccttcctg gttccagtgt ggtgttccac tggcctcgga atgactatga tcagctattg
    11881 tgtgtcagac tgatggacgt tcccaattgt atttggtctg gaggctttga agtcaacaag
    11941 aataattcct tccatatcaa catgagggat accttgggaa aatgcttctt cctacgagtg
    12001 gaaattactc tccgaggagc tacgtatagg atctcattta gtgacacaga tcagttacct
    12061 cctcctttcc gaattgacaa cttttctaag gtcccggttg tctttactca gcatggcgta
    12121 gctgaaccca ggctccggac tgaagtgaag cccatgactt cattggatta tgcctgggac
    12181 gaacccacct tgccaccttt tatcactctg actgttaaag gggcagggtc ctctgagatc
    12241 aactgcaaca tgaatgattt ccaggataat cggcagcttt attatgaaaa tttcatttac
    12301 attgctgcta catatacatt ctctggcttg caggagggaa caggcaggcc tgtggcttcc
    12361 aacaaggcca ttacctgtgc ggagctcgtt ttggatgtct cacccaagac acaaagagtc
    12421 attttaaaaa agaaggaacc aggaaagcgt tctcagctgt ggaggatgac aggaacagga
    12481 atgctggccc atgagggctc ctcagttcct cacaatccca ataagccctc agccgcccgc
    12541 tccaccgagg ggtctgccat cttagatatt gctggtctcg ctgcagtgac tgacaacaga
    12601 tacgagccac tgatgctgag aaagcctgac cgcaggcgaa gcacaactca gacgtggagt
    12661 ttccgagaag gaaaactgac ctgtgggtta catgggttgg tcgtccaggc caaaggagga
    12721 ctttctggtt tgtttgatgg agctgaagtt gttcttggtc ctgacacttc catggagctt
    12781 ttggggccag ttccacctga acaacaattt attaatcaaa aaatgagacc tggttctgga
    12841 atgttatcca tcagagtcat cccagatgga ccaactagag cactccagat aacagatttc
    12901 tgccaccgga aaagcagccg ttcatatgaa gtggatgaac ttcctgtcac cgaacaagag
    12961 ctgcagaaat taaagaatcc agatacagag caggaattgg aagtgcttgt gaggttagaa
    13021 ggtggaattg ggttgtcctt aattaataaa gtcccagaag aactggtctt tgcaagtctt
    13081 acaggaatca atgtgcacta tacacagctg gcaaccagtc acatgcttga actcagcata
    13141 caggatgtac aggtggacaa tcagctcatt ggtaccacgc agcccttcat gctctatgtg
    13201 actcccctga gcaatgagaa tgaggtcatc gagaccggcc cagctgtgca agtcaacgca
    13261 gtgaagttcc ccagtaagag tgcactgacc aacatctaca agcatctgat gatcacagct
    13321 cagagattca cagtgcaaat tgaggagaaa ctgctcctca agctgctaag tttctttggc
    13381 tacgatcaag cagaatcaga ggtggaaaaa tatgatgaaa acctccatga aaagacagct
    13441 gagcaaggtg gaacaccaat ttgatctaga taactgatca taatcagcca taccacattt
    13501 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    13561 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    13621 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    13681 tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt
    13741 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
    13801 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
    13861 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
    13921 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
    13981 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
    14041 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
    14101 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
    14161 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
    14221 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
    14281 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
    14341 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
    14401 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    14461 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
    14521 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
    14581 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
    14641 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca
    14701 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
    14761 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
    14821 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
    14881 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
    14941 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
    15001 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
    15061 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
    15121 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
    15181 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
    15241 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
    15301 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
    15361 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
    15421 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
    15481 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
    15541 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
    15601 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg
    15661 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca
    15721 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg
    15781 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
    15841 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
    15901 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat
    15961 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    16021 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    16081 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    16141 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    16201 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    16261 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    16321 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    16381 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    16441 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    16501 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    16561 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    16621 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    16681 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    16741 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
