LOCUS       sequence-34625-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TRIM54
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             301..318
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(371..389)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      385..432
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      469..897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(556..575)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     766..787
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tattttgttt actttaagaa ggagatatac catgcatcat catcatcatc acagcagcgg
       61 cagagaaaac ttgtatttcc agggccagga gtcatccagg ccgctgcact ccaaggctga
      121 gcagcacctc atgtgcgagg agcatgaaga agagaagatc aatatttact gcctgagctg
      181 tgaggtgccc acctgctctc tctgcaaggt cttcggtgcc cacaaggact gtgaggtggc
      241 cccactgccc accatttaca aacgccagaa gtgatgacga agcttgcggc cgcactcgag
      301 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      361 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      421 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      481 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      541 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      601 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      661 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
      721 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
      781 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
      841 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatt
//
