LOCUS       sequence-34626-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHF20 (3Q1J)
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..69
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..102
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             372..389
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(442..460)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      456..503
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(627..646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     838..859
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gagaaattcc ctctgaatat tttgtttact ttaagaagga gatataccat gcatcatcat
       61 catcatcaca gcagcggcag agaaaacttg tatttccagg gcatgacaaa gcatccacct
      121 aacagacgag gaatcagctt tgaagtggga gcccagttgg aagcccggga ccgtttaaaa
      181 aactggtatc cagctcacat agaagacatt gactacgagg aaggaaaagt actcatccat
      241 ttcaagcgtt ggaaccatcg ttatgatgag tggttctgct gggacagtcc ttatttacgc
      301 cctttagaga aaatacagct gaggaaagag ggcttgcatg aatgatgacg aagcttgcgg
      361 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      421 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      481 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      781 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaaatagtg
      841 gactcttgtt ccaaactgga a
//
