LOCUS       sequence-34627-e         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PLXNA2
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             44..61
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             457..474
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(527..545)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      541..588
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      625..983
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(712..731)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     922..943
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctgaat attttgttta ctttaagaag gagatatacc atgcatcatc atcatcatca
       61 cagcagcggc agagaaaact tgtatttcca gggcctgatt cgtcagcaaa tcgaatacaa
      121 aactctgatt ctgaactgtg ttaatccgga taacgagaac tcgccggaaa tcccagtcaa
      181 agtgctgaac tgcgatacaa ttacgcaggt aaaagaaaaa atcctggacg cagtttataa
      241 aaatgtgcct tatagccagc gcccgcgtgc tgtcgatatg gacctggagt ggcgccaagg
      301 ccgtattgcg cgcgttgtac tgcaggatga agatattacc actaaaatcg agggtgactg
      361 gaaacgtctg aacacactga tgcattacca agtgtctgac cgctctgtcg ttgccctggt
      421 gccgaaatga tgacgaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      481 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      541 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      601 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      661 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      721 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      781 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      841 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      901 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
      961 tctcggtcta tctttttgat ttaagatt
//
