LOCUS       sequence-346287-       10792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Focal Adhesion marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10792)
  AUTHORS   Arts JJG, Mahlandt EK, Schimmel L, Gronloh MLB, van der Niet S, 
            Klein BJAM, Fernandez-Borja M, van Geemen D, Huveneers S, van 
            Rijssel J, Goedhart J, van Buul JD
  TITLE     Endothelial Focal Adhesions Are Functional Obstacles for Leukocytes 
            During Basolateral Crawling.
  JOURNAL   Front Immunol. 2021 May 18;12:667213. doi: 
            10.3389/fimmu.2021.667213. eCollection 2021.
  PUBMED    34084168
REFERENCE   2  (bases 1 to 10792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Immunol."; date: "2021-05-18"; volume: "12:667213. doi"; pages: " 
            10.3389/fimmu.2021.667213. eCollection 2021"
COMMENT     Sequence Label: mTurquoise2-Paxillin
FEATURES             Location/Qualifiers
     source          1..10792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      687..696
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(693..695,696..698,699..1409)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(738..759)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(999..1018)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1346..1367
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3310..3862
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3454..3471)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3681..3700
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3889..4488
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3889..3908)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4385..4405
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5380..5613
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5685..5806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5722..5741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5776..5795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5846..5981
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6001..6020)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6002..6020)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6030..6047)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6030..6046)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6039..6061)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6188..6643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6275..6294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6485..6506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6669..6773
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6774..6842,6843..7634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6992..7011)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7805..8393
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8294..8313
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8547..8564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8681..8702
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8717..8722,8723..8740,8741..8747)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8755..8771
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8760..8782
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8779..8795
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8779..8795
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8814..8834
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8816..8834
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8862..9088
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9089..9269
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9316..9441
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9934..10167
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10352..10396
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10658..10775
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagct agaggatccc
      601 ccgggctgca ggaattcggc gcgccacgcg taccgatatc cagcacagtg gcggccgctc
      661 gagcctctag acagcgctca ccggtcgcca ccatggtgag caagggcgag gagctgttca
      721 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      781 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      841 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgtcc tggggcgtgc
      901 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      961 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     1021 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1081 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac tttagcgaca
     1141 acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc
     1201 acaacatcga ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1261 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca
     1321 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1381 tcactctcgg catggacgag ctgtacaagt ccggactcag atctcgagct gcggccgccg
     1441 gatccaagct tatggacgac ctcgacgccc tgctggcgga cttggagtct accacctccc
     1501 acatctccaa acggcctgtg ttcttgtcgg aggagacccc ctactcatac ccaactggaa
     1561 accacacata ccaggagatt gccgtgccac cccccgtccc cccacccccg tccagcgagg
     1621 ccctcaatgg cacaatcctt gaccccttag accagtggca gcccagcggc tcccgattca
     1681 tccaccagca gcctcagtcc tcatcacctg tgtacggctc cagtgccaaa acttccagtg
     1741 tctccaaccc tcaggacagt gttggctctc cgtgctcccg agtgggtgag gaggagcacg
     1801 tctacagctt ccccaacaag cagaaatcag ctgagccttc acccaccgta atgagcacgt
     1861 ccctgggcag caacctttct gaactcgacc gcctgctgct ggaactgaac gctgtacagc
     1921 ataacccgcc aggcttccct gcagatgagg ccaactcaag ccccccgctt cctggggccc
     1981 tgagccccct ctatggtgtc ccagagacta acagcccctt gggaggcaaa gctgggcccc
     2041 tgacgaaaga gaagcctaag cggaatgggg gccggggcct ggaggacgtg cggcccagtg
     2101 tggagagtct cttggatgaa ctggagagct ccgtgcccag ccccgtccct gccatcactg
     2161 tgaaccaggg cgagatgagc agcccgcagc gcgtcacctc cacccaacag cagacacgca
     2221 tctcggcctc ctctgccacc agggagctgg acgagctgat ggcttcgctg tcggatttca
     2281 agttcatggc ccaggggaag acagggagca gctcaccccc tggggggccc ccgaagcccg
     2341 ggagccagct ggacagcatg ctggggagcc tgcagtctga cctgaacaag ctgggggtcg
     2401 ccacagtcgc caaaggagtc tgcggggcct gcaagaagcc catcgccggg caggttgtga
     2461 ccgccatggg gaagacgtgg caccccgagc acttcgtctg cacccactgc caggaggaga
     2521 tcggatcccg gaacttcttc gagcgggatg gacagcccta ctgtgaaaag gactaccaca
     2581 acctcttctc cccgcgctgc tactactgca acggccccat cctggataaa gtggtgacag
     2641 cccttgaccg gacgtggcac cctgaacact tcttctgtgc acagtgtgga gccttctttg
     2701 gtcccgaagg gttccacgag aaggacggca aggcctactg tcgcaaggac tacttcgaca
     2761 tgttcgcacc caagtgtggc ggctgcgccc gggccatcct ggagaactat atctcagccc
     2821 tcaacacgct gtggcatcct gagtgctttg tgtgccggga atgcttcacg ccattcgtga
     2881 acggcagctt cttcgagcac gacgggcagc cctactgtga ggtgcactac cacgagcggc
     2941 gcggctcgct gtgttctggc tgccagaagc ccatcaccgg ccgctgcatc accgccatgg
     3001 ccaagaagtt ccaccccgag cacttcgtct gtgccttctg cctcaagcag ctcaacaagg
     3061 gcaccttcaa ggagcagaac gacaagcctt actgtcagaa ctgcttcctc aagctcttct
     3121 gctaggctag cctagtgcca tttgttcagt ggttcgtagg gctttccccc actgtttggc
     3181 tttcagttat atggatgatg tggtattggg ggccaagtct gtacagcatc ttgagtccct
     3241 ttttaccgct gttaccaatt ttcttttgtc tttgggtaaa cccgggaatt ccgcccctct
     3301 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     3361 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     3421 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     3481 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     3541 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     3601 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     3661 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     3721 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     3781 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     3841 tttgaaaaac acgatgataa tatggatatg gccacaacgg tcgccaccat gaccgagtac
     3901 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3961 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4021 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4081 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     4141 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     4201 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4261 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4321 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4381 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     4441 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
     4501 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catgtattca atctaagcag
     4561 gctttcactt tctcgccaac ttacaaggcc tttctgtgta aacaatacct gaacctttac
     4621 cccgttgccc ggcaacggcc aggtctgtgc caagtgtttg ctgacgcaac ccccactggc
     4681 tggggcttgg tcatgggcca tcagcgcatg cgtggaacct tttcggctcc tctgccgatc
     4741 catactgcgg aactcctagc cgcttgtttt gctcgcagca ggtctggagc aaacattatc
     4801 gggactgata actctgttgt cctatcccgc aaatatacat cgtttccatg gctgctaggc
     4861 tgtgctgcca actggatcct gcgcgggacg tcctttgttt acgtcccgtc ggcgctgaat
     4921 cctgcggacg acccttctcg gggtcgcttg ggactctctc gtccccttct ccgtctgccg
     4981 ttccgaccga ccacggggcg cacctctctt tacgcggact ccccgtctgt gccttctcat
     5041 ctgccggacc gtgtgcactt cgcttcacct ctgcacgtcg catggagacc accgtgaacg
     5101 cccaccaaat attgcccaag gtcttacata agaggactct tggactctca gcaatgtcaa
     5161 cgaccgacct tgaggcatac ttcaaagact gtttgtttaa agactgggag gagttggggg
     5221 aggagattag gttaaaggtc tttgtactag gaggctgtag gcataaattg gtctgcgcac
     5281 cagcaccatg gcgcaatcac tagagcgggg tacctttaag accaatgact tacaaggcag
     5341 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5401 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5461 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5521 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5581 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     5641 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     5701 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5761 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     5821 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5881 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5941 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     6001 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     6061 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     6121 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     6181 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     6241 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     6301 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     6361 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     6421 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     6481 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6541 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6601 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     6661 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6721 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6781 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6841 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6901 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6961 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     7021 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     7081 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     7141 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     7201 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     7261 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     7321 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     7381 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     7441 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     7501 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7561 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7621 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7681 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7741 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7801 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7861 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7921 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7981 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8041 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8101 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8161 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8221 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8281 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8341 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8401 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8461 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8521 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8581 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8641 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     8701 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     8761 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     8821 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     8881 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     8941 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     9001 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     9061 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     9121 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9181 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9241 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     9301 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9361 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9421 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     9481 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     9541 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     9601 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     9661 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     9721 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     9781 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     9841 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     9901 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     9961 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10021 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10081 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10141 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10201 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10261 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    10321 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10381 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10441 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    10501 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    10561 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    10621 atccattcga ttagtgaacg gatctcgacg gtatcggttt taaaagaaaa ggggggattg
    10681 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    10741 aattacaaaa acaaattaca aaaattcaaa attttatcga taagcttggg ag
//
