LOCUS       sequence-346290-        9595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of active RhoA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Mahlandt EK, Arts JJG, van der Meer WJ, van der Linden FH, Tol S, 
            van Buul JD, Gadella TWJ, Goedhart J
  TITLE     Visualizing endogenous Rho activity with an improved 
            localization-based, genetically encoded biosensor.
  JOURNAL   J Cell Sci. 2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823.
  PUBMED    34357388
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2021 Aug 6. pii: 271216. doi: 10.1242/jcs.258823."
COMMENT     Sequence Label: dimericTomato-2xrGBD
FEATURES             Location/Qualifiers
     source          1..9595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      807..816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             813..1511
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELY"
     primer_bind     complement(946..964)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1214..1233
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1408..1431
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    2096..2663
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2113..2665
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2257..2274)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2484..2503
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2692..3291
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2692..2711)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3188..3208
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4183..4416
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4488..4609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4525..4544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4579..4598
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4649..4784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4804..4823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4805..4823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4833..4850)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4833..4849)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4842..4864)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4991..5446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5078..5097)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5288..5309
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5472..5576
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5577..5645,5646..6437)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5795..5814)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6608..7196
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7097..7116
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7350..7367
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7484..7505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7520..7525,7526..7543,7544..7550)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7558..7574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7563..7585
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7582..7598
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7582..7598
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7617..7637
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7619..7637
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7665..7891
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7892..8072
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8119..8244
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8737..8970
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9155..9199
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9461..9578
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagct agaggatccc
      601 ccgggctgca ggaattcggc gcgccacgcg taccgatatc aactagaatg ctagtagtta
      661 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      721 ataacttacg gtaaatggcc cgcctgggag gtctatataa gcagagctgg tttagtgaac
      781 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggtcatca
      841 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      901 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      961 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     1021 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     1081 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1141 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1201 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1261 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1321 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1381 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     1441 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     1501 acgagctgta catccccctc gagtccatcc tggaggacct caatatgctc tacatccggc
     1561 agatggcact cagcctggag gacacagagc tgcagaggaa actagatcat gagatccgga
     1621 tgagggatgg ggcctgcaag ctgctggcag cctgctccca gcgagagcag gctctggaag
     1681 ccaccaagag cctgctggtg tgcaacagcc gtattctcag ctacatgggt gagctgcagc
     1741 ggcgaaagga ggcccaggtg ctggagaaga caggcaggcg tcgagttatc ctggaggacc
     1801 tcaatatgct ctacatccgg cagatggcac tcagcctgga ggacacagag ctgcagagga
     1861 aactagatca tgagatccgg atgagggatg gggcctgcaa gctgctggca gcctgctccc
     1921 agcgagagca ggctctggaa gccaccaaga gcctgctggt gtgcaacagc cgtattctca
     1981 gctacatggg tgagctgcag cggcgaaagg aggcccaggt gctggagaag acaggctcta
     2041 gataactgat cataatcagc catgctagca acggtttccc tctagcggga tcaattccgc
     2101 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     2161 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     2221 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     2281 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     2341 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     2401 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     2461 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     2521 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     2581 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     2641 tcctttgaaa aacacgatga taatatggat atggccacaa cggtcgccac catgaccgag
     2701 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     2761 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     2821 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     2881 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     2941 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     3001 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     3061 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     3121 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     3181 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     3241 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     3301 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatgtat tcaatctaag
     3361 caggctttca ctttctcgcc aacttacaag gcctttctgt gtaaacaata cctgaacctt
     3421 taccccgttg cccggcaacg gccaggtctg tgccaagtgt ttgctgacgc aacccccact
     3481 ggctggggct tggtcatggg ccatcagcgc atgcgtggaa ccttttcggc tcctctgccg
     3541 atccatactg cggaactcct agccgcttgt tttgctcgca gcaggtctgg agcaaacatt
     3601 atcgggactg ataactctgt tgtcctatcc cgcaaatata catcgtttcc atggctgcta
     3661 ggctgtgctg ccaactggat cctgcgcggg acgtcctttg tttacgtccc gtcggcgctg
     3721 aatcctgcgg acgacccttc tcggggtcgc ttgggactct ctcgtcccct tctccgtctg
     3781 ccgttccgac cgaccacggg gcgcacctct ctttacgcgg actccccgtc tgtgccttct
     3841 catctgccgg accgtgtgca cttcgcttca cctctgcacg tcgcatggag accaccgtga
     3901 acgcccacca aatattgccc aaggtcttac ataagaggac tcttggactc tcagcaatgt
     3961 caacgaccga ccttgaggca tacttcaaag actgtttgtt taaagactgg gaggagttgg
     4021 gggaggagat taggttaaag gtctttgtac taggaggctg taggcataaa ttggtctgcg
     4081 caccagcacc atggcgcaat cactagagcg gggtaccttt aagaccaatg acttacaagg
     4141 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4201 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4261 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4321 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4381 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     4441 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4501 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4561 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4621 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4801 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4861 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4921 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4981 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     5041 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     5101 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     5161 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     5221 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     5281 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     5341 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     5401 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     5461 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5521 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5581 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5641 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5701 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5761 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5821 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5881 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5941 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6001 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6061 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6121 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6181 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6241 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6301 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6361 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6421 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6481 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6541 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6601 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6661 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6721 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6781 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6841 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6901 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6961 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7021 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7081 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7141 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7201 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7261 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7321 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7381 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7441 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7501 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7561 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7621 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7681 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     7741 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7801 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7861 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7921 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7981 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8041 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8101 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8161 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8221 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8281 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8341 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     8401 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8461 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8521 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8581 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8641 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8701 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8761 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8821 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8881 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8941 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     9001 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     9061 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     9121 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     9181 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     9241 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     9301 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     9361 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     9421 cagatccatt cgattagtga acggatctcg acggtatcgg ttttaaaaga aaagggggga
     9481 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     9541 aagaattaca aaaacaaatt acaaaaattc aaaattttat cgataagctt gggag
//
