LOCUS       sequence-346291-        9334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of active Cdc42.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Mahlandt EK, Kreider-Letterman G, Chertkova AO, Garcia-Mata R, 
            Goedhart J
  TITLE     Cell-based optimization and characterization of genetically encoded 
            location-based biosensors for Cdc42 or Rac activity.
  JOURNAL   J Cell Sci. 2023 May 15;136(10):jcs260802. doi: 10.1242/jcs.260802. 
            Epub 2023 May 25.
  PUBMED    37226883
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-05-15"; volume: "136(10):jcs260802. doi"; pages: 
            " 10.1242/jcs.260802. Epub 2023 May 25"
COMMENT     Sequence Label: dimericTomato-1xwGBD
FEATURES             Location/Qualifiers
     source          1..9334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      807..816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             813..1514
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(946..964)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1214..1233
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1408..1431
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    1835..2402
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1852..2404
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1996..2013)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2223..2242
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2431..3030
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2431..2450)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2927..2947
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3922..4155
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4227..4348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4264..4283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4318..4337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4388..4523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4543..4562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4544..4562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4572..4589)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4572..4588)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4581..4603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4730..5185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4817..4836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5027..5048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5211..5315
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5316..5384,5385..6176)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5534..5553)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6347..6935
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6836..6855
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7089..7106
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7223..7244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7259..7264,7265..7282,7283..7289)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7297..7313
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7302..7324
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7321..7337
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7321..7337
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7356..7376
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7358..7376
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7404..7630
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7631..7811
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7858..7983
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8476..8709
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8894..8938
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9200..9317
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgttt tgacctccat agaagacacc gactctagct agaggatccc
      601 ccgggctgca ggaattcggc gcgccacgcg taccgatatc aactagaatg ctagtagtta
      661 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      721 ataacttacg gtaaatggcc cgcctgggag gtctatataa gcagagctgg tttagtgaac
      781 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggtcatca
      841 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      901 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      961 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     1021 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     1081 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     1141 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     1201 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     1261 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     1321 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     1381 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     1441 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg tacggcatgg
     1501 acgagctgta caagtccgga cctagcccag ctgataagaa acgctcaggg aagaagaaga
     1561 tcagcaaagc tgatattggt gcacccagtg gattcaagca tgtcagccac gtggggtggg
     1621 acccccagaa tggatttgac gtgaacaacc tcgacccaga tctgcggagt ctgttctcca
     1681 gggcaggaat cagcgaggcc cagctcaccg acgccgagac ctctaaactt atctacgact
     1741 tcattgagga ccagggtggg ctggaggctg tgcggcagga gatgaggcgc caggagttct
     1801 aggctagcaa cggtttccct ctagcgggat caattccgcc ccccccccct aacgttactg
     1861 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     1921 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     1981 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     2041 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     2101 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     2161 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     2221 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     2281 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     2341 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     2401 aatatggata tggccacaac ggtcgccacc atgaccgagt acaagcccac ggtgcgcctc
     2461 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     2521 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     2581 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     2641 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     2701 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     2761 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     2821 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     2881 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     2941 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     3001 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     3061 gaaaggagcg cacgacccca tgcatgtatt caatctaagc aggctttcac tttctcgcca
     3121 acttacaagg cctttctgtg taaacaatac ctgaaccttt accccgttgc ccggcaacgg
     3181 ccaggtctgt gccaagtgtt tgctgacgca acccccactg gctggggctt ggtcatgggc
     3241 catcagcgca tgcgtggaac cttttcggct cctctgccga tccatactgc ggaactccta
     3301 gccgcttgtt ttgctcgcag caggtctgga gcaaacatta tcgggactga taactctgtt
     3361 gtcctatccc gcaaatatac atcgtttcca tggctgctag gctgtgctgc caactggatc
     3421 ctgcgcggga cgtcctttgt ttacgtcccg tcggcgctga atcctgcgga cgacccttct
     3481 cggggtcgct tgggactctc tcgtcccctt ctccgtctgc cgttccgacc gaccacgggg
     3541 cgcacctctc tttacgcgga ctccccgtct gtgccttctc atctgccgga ccgtgtgcac
     3601 ttcgcttcac ctctgcacgt cgcatggaga ccaccgtgaa cgcccaccaa atattgccca
     3661 aggtcttaca taagaggact cttggactct cagcaatgtc aacgaccgac cttgaggcat
     3721 acttcaaaga ctgtttgttt aaagactggg aggagttggg ggaggagatt aggttaaagg
     3781 tctttgtact aggaggctgt aggcataaat tggtctgcgc accagcacca tggcgcaatc
     3841 actagagcgg ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     3901 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     3961 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4021 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4081 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4141 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     4201 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     4261 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4321 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     4381 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4441 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4501 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4561 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4621 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4681 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4741 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     5161 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5221 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5281 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5341 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5401 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5461 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5521 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5581 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5641 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5701 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5761 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5821 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5881 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5941 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6001 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6061 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6121 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6181 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6241 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6301 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6361 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6421 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6481 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6541 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6601 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6661 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6721 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6781 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6841 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6901 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6961 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7021 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7081 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7141 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     7201 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     7261 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     7321 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     7381 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     7441 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     7501 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7561 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7621 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7681 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7741 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7801 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7861 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7921 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7981 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     8041 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     8101 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     8161 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     8221 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     8281 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     8341 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8401 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8461 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8521 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8581 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8641 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8701 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8761 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8821 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8881 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8941 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     9001 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     9061 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     9121 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     9181 cggatctcga cggtatcggt tttaaaagaa aaggggggat tggggggtac agtgcagggg
     9241 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9301 caaaaattca aaattttatc gataagcttg ggag
//
