LOCUS       sequence-346292-        9606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of  Myc-tagged mouse Emd.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Cheng LC, Zhang X, Abhinav K, Nguyen JA, Baboo S, Martinez-Bartolome
            S, Branon TC, Ting AY, Loose E, Yates JR 3rd, Gerace L
  TITLE     Shared and Distinctive Neighborhoods of Emerin and Lamin B Receptor 
            Revealed by Proximity Labeling and Quantitative Proteomics.
  JOURNAL   J Proteome Res. 2022 Sep 2;21(9):2197-2210. doi: 
            10.1021/acs.jproteome.2c00281. Epub 2022 Aug 16.
  PUBMED    35972904
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jproteome.2c00281"; journalName: "J Proteome Res"; 
            date: "2022-09-2- 2"; volume: "21"; issue: "9"; pages: "2197-2210"
COMMENT     Sequence Label: pLV Myc-emerin
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    732..866
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(756..775)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     810..829
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        914..1018
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1019..1087,1088..1879)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1237..1256)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2050..2638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2539..2558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2792..2809
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2926..2947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2962..2967,2968..2985,2986..2992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3000..3016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3005..3027
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3024..3040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3024..3040
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             3168..3801
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4008..4596
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4114..4125
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4523..4543
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4620..5219)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4703..4723)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5200..5219
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     misc_feature    5222..5771
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5381..5400)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5610..5627
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(6751..6780)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      6780..6789
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          6800..7738
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6823..6843)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(7739..7950)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    8028..8143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8406..8450)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8635..8868
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9365..9490
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(9537..9606,1..564)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
       61 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc
      121 aaaaagcagc tgcttatatg caggatctga gggctcgcca ctccccagtc ccgcccaggc
      181 cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta gcaagctcga tatcagcagt
      241 tcttgaagta ctccggatgc agctctcggg ccacgtgatg aaatgctagg cggctgtcaa
      301 acctccactc taacacttct ctctccgggt catccatccc atgcaggctc acagggtgta
      361 acaagctggt gttctctcct ttattggcct cttctacctt atctggctca actggtacta
      421 gcttgtagca ccatccaaag gtcagtggat atctgacccc tggccctggt gtgtagttct
      481 gctaatcagg gaagtagcct tgtgtgtggt agatccacag atcaaggata tcttgtcttc
      541 tttgggagtg aattagccct tccaactact aagtttgtag tacatattta acaaatacaa
      601 tttctttaaa atgaaaataa ttcagaggaa tcacaggttt agagtaaatg aaaccacagg
      661 taattggcag tggtaatagg gtatggggtg ggaagtttgg gatgattttg gttagcttga
      721 gttatccagt tgatccagac atgataagat acattgatga gtttggacaa accacaacta
      781 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
      841 ccattataag ctgcaataaa caagttgatc tcccgatccg tcgacgtcag gtggcacttt
      901 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      961 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1021 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1081 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1141 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1201 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1261 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1321 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1381 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1441 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1501 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1561 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1621 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1681 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1741 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1801 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1861 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1921 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2161 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2641 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2701 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2761 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2821 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     2881 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2941 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3001 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcta
     3061 gctagaggtc gacggtaaac gctagcaatg tcaaggcctc tcactctctg atattcattt
     3121 ctttgcaagt tataaatact gaataataag atgacatgaa ctactactgc tagagatttt
     3181 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     3241 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     3301 ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa
     3361 aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac
     3421 gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat cagcagttct
     3481 tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg ctgtcaaacc
     3541 tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca gggtgtaaca
     3601 agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact ggtactagct
     3661 tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg tagttctgct
     3721 aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct tgtcttcgtt
     3781 gggagtgaat tagcccttcc agtcccctct tttcttttaa aaagtggcta agatctacag
     3841 ctgccttgta agtcattggt cttaaaggta cctgaggtgt gactggaaaa cccacctcct
     3901 cctcctcttg tgcttctagc caggcacaat cagcattggt agctgctgta ttgctacttg
     3961 tgattgctcc atgtttttct aggtctcgac tgcagaatta attccaggcg gggaggcggc
     4021 ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag gccgcagagc
     4081 cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga cgtagcagaa
     4141 ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccatggaa aggacgtcag
     4201 cttccccgac aacaccacgg aattgtcagt gcccaacagc cgagcccctg tccagcagcg
     4261 ggcaaggcag gcggcgatga gttccgccgt ggcaataggg agggggaaag cgaaagtccc
     4321 ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac
     4381 agtgcacacc acgccacgtt gcctgacaac gggccacaac tcctcataaa gagacagcaa
     4441 ccaggattta tacaaggagg agaaaatgaa agccatacgg gaagcaatag catgatacaa
     4501 aggcattaaa gcagcgtatc cacatagcgt aaaaggagca acatagttaa gaataccagt
     4561 caatctttca caaattttgt aatccagagg ttgattgttc cagacgcgcc ccgcccgggt
     4621 caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg
     4681 gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc
     4741 aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg
     4801 cccagaccct tgccctggtg gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc
     4861 tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa
     4921 ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc
     4981 tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg cgacccacac cttgccgatg
     5041 tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg
     5101 tccggatcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt
     5161 acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg
     5221 gtattatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt
     5281 ttttaacctc gactaaacac atgtaaagca tgtgcaccga ggccccagat cagatcccat
     5341 acaatggggt accttctggg catccttcag ccccttgttg aatacgcttg aggagagcca
     5401 tttgactctt tccacaacta tccaactcac aacgtggcac tggggttgtg ccgcctttgc
     5461 aggtgtatct tatacacgtg gcttttggcc gcagaggcac ctgtcgccag gtggggggtt
     5521 ccgctgcctg caaagggtcg ctacagacgt tgtttgtctt caagaagctt ccagaggaac
     5581 tgcttccttc acgacattca acagaccttg cattcctttg gcgagagggg aaagacccct
     5641 aggaatgctc gtcaagaaga cagggccagg tttccgggcc ctcacattgc caaaagacgg
     5701 caatatggtg gaaaataaca tatagacaaa cgcacaccgg ccttattcca agcggcttcg
     5761 gccagtaacg ttaggggggg ggggcggaat tgatcccgct agagggaaac cgttgctagc
     5821 ttaagttagt taacaattgt taccgcggcc cgtcgactct agagcggccg ccctcgagga
     5881 ggaattcacg cgtttcgaag tttaaactta aacgggatcc atccagaagg ggttgccttc
     5941 ttcagcttgt atggaatagt aaacaaagag cagaaaggca gcaaagacca ggaagagtaa
     6001 cagctggccc cagagtggga cctgtcgatc ctggcccaga gcagcagcag gggcctgctt
     6061 ctctggccga atggcacgtc gggtaagcca tgaagaggga gaagatgaag aggaggacac
     6121 agaagaggtg gaggatgtag gataatatga taagcccaga gagctcctgg agacattgga
     6181 aatggggcgg tagtgtgcga tgctctggta ggcactgtct tggccataga tgaggcgctc
     6241 cctatccttg ccttcctctt ctaaagaaga gaaaatgtca tcacgcacct ggtgatggaa
     6301 ggtatcagca tctacaagtg aggtccccgg ctggcggaag ctcttggaca tgcccacaga
     6361 ctcaggctcc ccgtatgtct tggtggtcaa ataactctcc tcatagtagt catcattata
     6421 gtccttgctc tggtaaagta aggcgtcttc ttttttgggc agatcataca tgtctgagtc
     6481 cacggcggct gaatccaagt ctgagaactg ataggagaat gaagaagatg acgagttggg
     6541 gggtaagagt ctccttctct gggtctcgta ctcgaagatt ttcttttcgt agagcttgcg
     6601 agtggaaccc acaataggcc catgagggat gttgtactgg cgtagcactg cggccagctc
     6661 agtatcggac aaaaccgcat agtcgtcatg gccggccgat atccccgacc cagatccgcc
     6721 tgatccagag ccacccgacc ctgatccgcc gagatcctct tctgagatga gtttttgttc
     6781 catggcggcc tcacgacacc tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct
     6841 tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc caaggagaat tacatcaagt
     6901 gccaagctgg cctaacttca gtctccaccc actcagtgtg gggaaactcc atcgcataaa
     6961 acccctcccc ccaacctaaa gacgacgtac tccaaaagct cgagaactaa tcgaggtgcc
     7021 tggacggcgc ccggtactcc gtggagtcac atgaagcgac ggctgaggac ggaaaggccc
     7081 ttttcctttg tgtgggtgac tcacccgccc gctctcccga gcgccgcgtc ctccattttg
     7141 agctccctgc agcagggccg ggaagcggcc atctttccgc tcacgcaact ggtgccgacc
     7201 gggccagcct tgccgcccag ggcggggcga tacacggcgg cgcgaggcca ggcaccagag
     7261 caggccggcc agcttgagac tacccccgtc cgattctcgg tggccgcgct cgcaggcccc
     7321 gcctcgccga acatgtgcgc tgggacgcac gggccccgtc gccgcccgcg gccccaaaaa
     7381 ccgaaatacc agtgtgcaga tcttggcccg catttacaag actatcttgc cagaaaaaaa
     7441 gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga cttatcgaaa gcagcgagac
     7501 aggcgcgaag gtgccaccag attcgcacgc ggcggcccca gcgcccaggc caggcctcaa
     7561 ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct cgaactctcc cacccacttc
     7621 caacccgaag ctcgggatca agaatcacgt actgcagcca ggtggaagta attcaaggca
     7681 cgcaagggcc ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct
     7741 gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat
     7801 acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt
     7861 ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct
     7921 cggggactgt gggcgatgtg cgctctgccc actgacgggc acctcgagtg aagacgaaag
     7981 ggcctcatcg ataaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt
     8041 ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc
     8101 tttcccctgc actgtacccc ccaatccccc cttttctttt aaaggcgata ccgtcgagat
     8161 ccgttcacta atcgaatgga tctgtctctg tctctctctc caccttcttc ttctattcct
     8221 tcgggcctgt cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
     8281 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
     8341 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
     8401 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
     8461 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
     8521 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
     8581 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
     8641 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
     8701 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
     8761 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
     8821 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
     8881 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     8941 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     9001 ccaggtctga agatcagcgg ccggccgctt gctgtgcggt ggtcttactt ttgttttgct
     9061 cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac
     9121 aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg
     9181 atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac aggccaggat
     9241 taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt
     9301 tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa
     9361 tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg
     9421 tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc
     9481 gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct
     9541 agagattttc cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa
     9601 cagacg
//
