LOCUS       sequence-34630-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: USP15
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..95
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             779..796
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(849..867)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc catcatcatc
       61 atcatcacag cagcggcctg gttccgcgtg gtagtgcggc ggatctggac acccagcggt
      121 ctgacatcgc gacgctgctc aaaacctcgc tccggaaagg ggacacctgg tacctagtcg
      181 atagtcgctg gttcaaacag tggaaaaaat atgttggctt tgacagttgg gacaaatacc
      241 agatgggaga tcaaaatgtg tatcctggac ccattgataa ctctggactt ctcaaagatg
      301 gtgatgccca gtcacttaag gaacacctta ttgatgaatt ggattacata ctgttgccaa
      361 ctgaaggttg gaataaactt gtcagctggt acacattgat ggaaggtcaa gagccaatag
      421 cacgaaaggt ggttgaacag ggtatgtttg taaagcactg caaagtagaa gtatatctca
      481 cagaattgaa gctatgtgaa aatggaaaca tgaataatgt tgtaactcga agatttagca
      541 aagctgacac aatagataca attgaaaagg aaataagaaa aatcttcagt attccagatg
      601 aaaaggagac cagattgtgg aacaaataca tgagtaacac atttgaacca ctgaataaac
      661 cagacagcac cattcaggat gctggtttat accaaggaca ggtattagtg atagaacaga
      721 aaaatgaaga tggaacatgg ccaaggggtt gatgacgaag cttgcggccg cactcgagca
      781 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      841 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaacg ggtcttgagg
      901 ggtttttgct gaaggagaac tatatccgga
//
