LOCUS       sequence-346303-       10388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT 
            fusion driven by CAG promoter and tagBFP driven by CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Kong X, Wang Z, Zhang R, Wang X, Zhou Y, Shi L, Yang H
  TITLE     Precise genome editing without exogenous donor DNA via retron 
            editing system in human cells.
  JOURNAL   Protein Cell. 2021 Aug 17. pii: 10.1007/s13238-021-00862-7. doi: 
            10.1007/s13238-021-00862-7.
  PUBMED    34403072
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Cell. 2021 Aug 17. pii: 10.1007/s13238-021-00862-7. doi: 
            10.1007/s13238-021-00862-7."
COMMENT     Sequence Label: pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA
FEATURES             Location/Qualifiers
     source          1..10388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        118..403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     repeat_region   712..774
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(872..1460)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(952..971)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1631..2422,2423..2491))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2254..2273
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2492..2596)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    2623..2830
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(2831..3532)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(3561..3764)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3590..3610)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3765..4068
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   4126..4160
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    4525..4732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4721..4738
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4751..4771)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5858..9958)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(5858..9958)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(9983..10003)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      10006..10015
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          10050..10277
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
ORIGIN
        1 cccccccccc gcccccatcg ctgcacaaaa taattaaaaa ataaataaat acaaaattgg
       61 gggtggggag gggggggaga tggggagagt gaagcagaac gtggggctca cctcgaccat
      121 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      181 gtactgggca caatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      241 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      301 ttgacgtcaa tggaaagtcc ctattggcgt tactattgac gtcaatgggc gggggtcgtt
      361 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgggtacct ctagattaat
      421 taataagtat aatttgtttc tattatgtat aagttaagct aattacttat tttataatac
      481 aacatgactg tttttaaagt acaaaataag tttatttttg taaaagagag aatgtttaaa
      541 agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
      601 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
      661 aatatctatt caaattaata aataaacctc gatatacaga tcgataaaac acatgcgtca
      721 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaact
      781 cgagagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      841 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      901 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      961 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1021 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1081 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1141 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1201 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1261 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1321 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1381 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1441 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1501 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1561 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1621 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1681 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1741 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1801 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1861 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1921 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1981 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2041 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2101 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2161 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2221 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2281 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2341 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2401 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2461 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2521 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2581 aaataggggt tccgcgcaca tttccccgaa aagtgcggat cctccccagc atgcctgcta
     2641 ttctcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga
     2701 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg
     2761 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta
     2821 gaaggcacag ttaattcagc ttgtgcccca gtttgctagg gaggtcgcag tatctggcca
     2881 ctgccacctc gtgctgctcg acgtatgtct cgttgttggc ctccttgatt ctttccagtc
     2941 tgtagtccac atagtagacg ccaggcatct tgaggttctt agcgggtttc ttggatctat
     3001 atgtggtctt gatgtttgcg atcagatggc tcccgcccac gagcttcagg gccatgtcgt
     3061 ttctgccttc caggccgccg tcagcggggt acagtgtctc ggtgaaggcc tcccagccga
     3121 gtgttttctt ctgcatcaca gggccgttgg atgtgaagtt cacccctctg atcttgacgt
     3181 tgtagatgag gcagccgtcc tggaggctgg tgtcctgggt agcggtcagc acgcccccgt
     3241 cctcgtatgt ggtgactctc tcccatgtga agccctcagg gaaggactgc ttgaagaagt
     3301 cggggatgcc ctgggtgtgg ttgatgaagg tcttgctgcc gtagaggaag ctagtagcca
     3361 ggatgtcgaa ggcgaagggg agagggccgc cctcgaccac cttgattctc atggtctggg
     3421 tgccctcgta gggcttgcct tcgccctcgg atgtgcactt gaagtgatgg ttgtccacgg
     3481 tgccctccat atacagcttc atgtgcatgt tctccttaat cagctcgctc atgctagcgg
     3541 atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac acgcctaccg
     3601 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
     3661 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
     3721 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
     3781 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     3841 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     3901 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     3961 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     4021 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgtc tagagcaaaa
     4081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc cttaacccta gaaagatagt
     4141 ctgcgtaaaa ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa
     4201 tccgtcgctg tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt
     4261 gtcaatgcgg taagtgtcac tgattttgaa ctataacgac cgcgtgagtc aaaatgacgc
     4321 atgattatct tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc
     4381 atgttctact tacgtgataa cttattatat atatattttc ttgttataga tatcgtgact
     4441 aatatataat aaaagcatta cacgtcttga gcgattgtgt aggctggagc tgcttcgtct
     4501 agggcgcgcc gtcgacgcgg ccgctcccca gcatgcctgc tattctcttc ccaatcctcc
     4561 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
     4621 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
     4681 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
     4741 gaagctttta gactttcctc ttcttcttgg gctcgaactc gctgccgtcg gcggttcttt
     4801 ttgagccgcc agattccact ttgttgtgca ggttctggat ccatttgatc tcctgaaaat
     4861 actttctatt aagctttgtg tagaagtggg ggtcgatgtc cttagcatag gcgatgtagc
     4921 cgctgagctt gttgtggtcc tcgtccttca ggatgccctt gctcagcaga gacagatgca
     4981 gccggatctt atccttcatg gatctgtgca gtgtgatgtg aaaatcgtgg cacactttca
     5041 ggccggtcac cacgatgctg ccgccagagg cgctacagat cttgaatttc ttaatgttaa
     5101 tcttgaaatc aggtccgatt tccttcattg ttcttttgaa gcagtccagg atcagtttgc
     5161 tggcgccctt catgttggtg gacacgatga tgtcgtcggc gtatctggtg tatgtagcgt
     5221 tcagcttgtc gatggcgttc agcttctggg tcagcttttc atccagctct ctggcgacga
     5281 aattagcgat cagtggagat gtagggaagc cgataggcag ggtggaatcg ctgatgaagc
     5341 agatggtctt gatcagctgc agcagttctt tgtcgtactc ggtggtgaac tcgattctgt
     5401 cccggtatct tgtgaaggcg tactcgaagt cggtgaactt gatgctgggg aagaaatcct
     5461 tcaggtcgat cttcacgtag tacttgttct tgctctcggc gtgcagcagg gcgttagact
     5521 tgatggaccg gtttttcaca aaagcgtagg cggcattgtg cataggcaac ttgctgaaca
     5581 cgttgttcat cagccagtat tggatcagct taaccttaga gctagggtgg tagatggtcc
     5641 tcattccgcc ctttttcttg gcgatgtccc acttcttggg aggttcaggg cttctcatca
     5701 cctcgctggc aaagcccttg gtcatcagcg tctggctatc aatcaggctg tagatccggc
     5761 ttgacccccc gctgctgccc ccgctgcttt caggtgttgc gctctcgctt gtgccaggtg
     5821 tctcagagcc agaggagcct ccgctagatc ctccggagtc gcctcccagc tgagacaggt
     5881 cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct
     5941 ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag
     6001 gggctcccag attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg
     6061 gcttatcccg gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga
     6121 tcactctctt ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct
     6181 gttccacaaa cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt
     6241 ggctggccag gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct
     6301 gcagttcgcc ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt
     6361 acttaggcag cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccagaa
     6421 agtcgatggg attcttctcg aagctgcttc tttccatgat ggtgatcccc agcagctctt
     6481 tcacactctt cagtttcttg gacttgccct tttccacttt ggccaccacc agcacagaat
     6541 aggccacggt ggggctgtcg aagccgccgt acttcttagg gtcccagtcc ttctttctgg
     6601 cgatcagctt atcgctgttc ctcttgggca ggatagactc tttgctgaag ccgcctgtct
     6661 gcacctcggt ctttttcacg atattcactt ggggcatgct cagcactttc cgcacggtgg
     6721 caaaatcccg gcccttatcc cacacgatct ccccggtttc gccgtttgtc tcgatcagag
     6781 gccgcttccg gatctcgccg ttggccaggg taatctcggt cttgaaaaag ttcatgatgt
     6841 tgctgtagaa gaagtacttg gcggtagcct tgccgatttc ctgctcgctc ttggcgatca
     6901 tcttccgcac gtcgtacacc ttgtagtcgc cgtacacgaa ctcgctttcc agcttagggt
     6961 actttttgat cagggcggtt cccacgacgg cgttcaggta ggcgtcgtgg gcgtggtggt
     7021 agttgttgat ctcgcgcact ttgtaaaact ggaaatcctt ccggaaatcg gacaccagct
     7081 tggacttcag ggtgatcact ttcacttccc ggatcagctt gtcattctcg tcgtacttag
     7141 tgttcatccg ggagtccagg atctgtgcca cgtgctttgt gatctgccgg gtttccacca
     7201 gctgtctctt gatgaagccg gccttatcca gttcgctcag gccgcctctc tcggccttgg
     7261 tcagattgtc gaactttctc tgggtaatca gcttggcgtt cagcagctgc cgccagtagt
     7321 tcttcatctt cttcacgacc tcttcggagg gcacgttgtc gctcttgccc cggttcttgt
     7381 cgcttctggt cagcaccttg ttgtcgatgg agtcgtcctt cagaaagctc tgaggcacga
     7441 tatggtccac atcgtagtcg gacagccggt tgatgtccag ttcctggtcc acgtacatat
     7501 cccgcccatt ctgcaggtag tacaggtaca gcttctcgtt ctgcagctgg gtgttttcca
     7561 cggggtgttc tttcaggatc tggctgccca gctctttgat gccctcttcg atccgcttca
     7621 ttctctcgcg gctgttcttc tgtcccttct gggtggtctg gttctctctg gccatttcga
     7681 tcacgatgtt ctcgggcttg tgccggccca tcactttcac gagctcgtcc accaccttca
     7741 ctgtctgcag gatgcccttc ttaatggcgg ggctgccggc cagattggca atgtgctcgt
     7801 gcaggctatc gccctggccg gacacctggg ctttctggat gtcctcttta aaggtcaggc
     7861 tgtcgtcgtg gatcagctgc atgaagtttc tgttggcgaa gccgtcggac ttcaggaaat
     7921 ccaggattgt cttgccggac tgcttgtccc ggatgccgtt gatcagcttc cggctcagcc
     7981 tgccccagcc ggtgtatctc cgccgcttca gctgcttcat cactttgtcg tcgaacaggt
     8041 gggcataggt tttcagccgt tcctcgatca tctctctgtc ctcaaacagt gtcagggtca
     8101 gcacgatatc ttccagaatg tcctcgtttt cctcattgtc caggaagtcc ttgtccttga
     8161 taattttcag cagatcgtgg tatgtgccca gggaggcgtt gaaccgatct tccacgccgg
     8221 agatttccac ggagtcgaag cactcgattt tcttgaagta gtcctctttc agctgcttca
     8281 cggtcacttt ccggttggtc ttgaacagca ggtccacgat ggcctttttc tgctcgccgc
     8341 tcaggaaggc gggctttctc attccctcgg tcacgtattt cactttggtc agctcgttat
     8401 acacggtgaa gtactcgtac agcaggctgt gcttgggcag caccttctcg ttgggcaggt
     8461 tcttatcgaa gttggtcatc cgctcgatga agctctgggc ggaagcgccc ttgtccacca
     8521 cttcctcgaa gttccagggg gtgatggttt cctcgctctt tctggtcatc caggcgaatc
     8581 tgctgtttcc cctggccaga gggcccacgt agtaggggat gcggaaggtc aggatcttct
     8641 cgatcttttc ccggttgtcc ttcaggaatg ggtaaaaatc ttcctgccgc cgcagaatgg
     8701 cgtgcagctc tcccaggtgg atctggtggg ggatgctgcc gttgtcgaag gtccgctgct
     8761 tccgcagcag gtcctctctg ttcagcttca cgagcagttc ctcggtgccg tccatctttt
     8821 ccaggatggg cttgatgaac ttgtagaact cttcctggct ggctccgccg tcaatgtagc
     8881 cggcgtagcc gttcttgctc tggtcgaaga aaatctcttt gtacttctca ggcagctgct
     8941 gccgcacgag agctttcagc agggtcaggt cctggtggtg ctcgtcgtat ctcttgatca
     9001 tagaggcgct caggggggcc ttggtgatct cggtgttcac tctcaggatg tcgctcagca
     9061 ggatggcgtc ggacaggttc ttggcggcca gaaacaggtc ggcgtactgg tcgccgatct
     9121 gggccagcag gttgtccagg tcgtcgtcgt aggtgtcctt gctcagctgc agtttggcat
     9181 cctcggccag gtcgaagttg ctcttgaagt tgggggtcag gcccaggctc agggcaatca
     9241 ggtttccgaa caggccattc ttcttctcgc cgggcagctg ggcgatcaga ttttccagcc
     9301 gtctgctctt gctcagtctg gcagacagga tggccttggc gtccacgccg ctggcgttga
     9361 tggggttttc ctcgaacagc tggttgtagg tctgcaccag ctggatgaac agcttgtcca
     9421 cgtcgctgtt gtcggggttc aggtcgccct cgatcaggaa gtggccccgg aacttgatca
     9481 tgtgggccag ggccagatag atcagccgca ggtcggcctt gtcggtgctg tccaccagtt
     9541 tctttctcag gtggtagatg gtggggtact tctcgtggta ggccacctcg tccacgatgt
     9601 tgccgaagat ggggtgccgc tcgtgcttct tatcctcttc caccaggaag gactcttcca
     9661 gtctgtggaa gaagctgtcg tccaccttgg ccatctcgtt gctgaagatc tcttgcagat
     9721 agcagatccg gttcttccgt ctggtgtatc ttcttctggc ggttctcttc agccgggtgg
     9781 cctcggctgt ttcgccgctg tcgaacagca gggctccgat caggttcttc ttgatgctgt
     9841 gccggtcggt gttgcccagc accttgaatt tcttgctggg caccttgtac tcgtcggtga
     9901 tcacggccca gcccacagag ttggtgccga tgtccaggcc gatgctgtac ttcttgtcgg
     9961 ctgctgggac tccgtggata ccgaccttcc gcttcttctt tggggccatg gtggcgtcga
    10021 ctagcggctt cggtacccac cggtccaacc tgaaaaaaag tgatttcagg caggtgctcc
    10081 aggtaattaa acattaatac cccaccaacc aaccatccct taaaccctta cctcttgctc
    10141 agctaattac agcccggagg agaagggccg tcccgcccgc tcacctgtgg gagtaacgcg
    10201 gtcagtcaga gccggggcgg gcggcgcgag gcggcggcgg agcggggcac ggggcgaagg
    10261 cagcgcgcag cgactcccgc ccgccgcgcg cttcgctttt tatagggccg ccgccgccgc
    10321 cgcctcgcca taaaaggaaa ctttcggagc gcgccgctct gattggctgc cgccgcacct
    10381 ctccgcct
//
