LOCUS       sequence-346309-       11059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-331-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11059)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 11059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-331-5p
FEATURES             Location/Qualifiers
     source          1..11059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(87..675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(167..186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(846..1637,1638..1706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1469..1488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1707..1778)
                     /gene="bla"
                     /label=AmpR promoter
     protein_bind    1795..1832
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1863..2462
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1863..1882)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2359..2379
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    2628..2683
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    3662..3682
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        3731..4909
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3962..4900
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    4926..4950
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4961..4970
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4967..5659
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    complement(5682..5706)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5995..6050
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    6434..6459
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    7131..7151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        7200..8378
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7431..8369
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    8395..8419
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8424..8433
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8430..8432,8433..8435,8436..9149)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(8475..8496)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(9151..9175)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9330..9385
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9746..9762)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9746..9762)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9759..9781)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9770..9786
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9794..9800,9801..9818,9819..9824))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9839..9860
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10074..10093)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             10085..10894
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(10152..10171)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aattgagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
       61 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
      481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
      541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
      601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
      661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
      721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
      781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
      841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
      901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
      961 ggccccagtg ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca
     1021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     1501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     1561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     1621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     1681 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     1741 caaggttatt gtctcatgag cggatacata tttgaatgta ccgaatttct cgagggcttg
     1801 tcgacgacgg cggtctccgt cgtcaggatc atgaattcga ccgcctgtct caaggtgcca
     1861 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg
     1921 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg
     1981 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     2041 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     2101 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     2161 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     2221 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     2281 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     2341 tggagacatc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     2401 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     2461 gagctttgag actacgcgtg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
     2521 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
     2581 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
     2641 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2701 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
     2761 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
     2821 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     2881 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     2941 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     3001 atggagatcc ctcgacctgc agcccaagct tgaattctct agaattaccc tgttatccct
     3061 atgcttggtt taccgagctc ttattggttt tcaaacttca ttgactgtgc ccaacgcgcc
     3121 tgggagctca cggggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
     3181 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     3241 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     3301 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     3361 aaagctaggc tgaaagaccg gttcttagaa tgacacgcgc ctgggagctc acggggacag
     3421 ccccccccca aagcccccag ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg
     3481 agccgcccgg ggctccgctc cggtccggcg ctccccccgc atccccgagc cggcagcgtg
     3541 cggggacagc ccgggcacgg ggaaggtggc acgggatcgc tttcctctga acgcttctcg
     3601 ctgctctttg agcctgcaga cacctggggg gatacgggga aaaagctaac ttctgagacg
     3661 acaactttgt atagaaaagt tggctccgaa ttcgcccttg acagcagaga tccagtttgg
     3721 ttaattaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     3781 gaagttgtgg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     3841 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     3901 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     3961 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     4021 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     4081 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     4141 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     4201 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     4261 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     4321 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     4381 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     4441 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     4501 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     4561 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     4621 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     4681 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     4741 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     4801 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     4861 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag aagggcgaat
     4921 tcgacccaag tttgtacaaa aaagcaggct ggatccaggt gccaccatgg tgagcgagct
     4981 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     5041 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     5101 ggcggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     5161 cggcagcaaa accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     5221 ccccgagggc ttcacatggg agagagtcac cacatacgaa gatgggggcg tgctgaccgc
     5281 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     5341 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac
     5401 cgagacactg taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct
     5461 cgtgggcggg ggccacctga tctgcaacct taagaccaca tacagatcca agaaacccgc
     5521 taagaacctc aagatgcccg gcgtctacta tgtggacagg agactggaaa gaatcaagga
     5581 ggccgacaaa gagacatacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     5641 ccctagcaaa ctggggcaca aacttaattg agaattctac cacccagctt tcttgtacaa
     5701 agtggtacgt ctcaagacca gaggtctctg cttaattcat aggatccctg ggaccatacc
     5761 tagggatccc tgggaccata cctagggatc cctgggacca tacctaggga tccctgggac
     5821 catacctagc aaccagaaga gacctgtgct tcactcctca ggtgcaggct gcctatcaga
     5881 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     5941 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     6001 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     6061 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     6121 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     6181 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     6241 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     6301 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     6361 ttctcttatg gagatcccga gcggtgcgtt tttatgcttg tagtattgta taatgttttt
     6421 aagatccaag cttctgacct cttctcttcc tcccacaggg cctcgagaca attgcatcga
     6481 tggtaccagt gaaagtctgc aatgaattcg agttggcgcg cctgtcattc taaatctctc
     6541 tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     6601 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     6661 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     6721 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggagggggct
     6781 gtccccgtga gctcccaggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt
     6841 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     6901 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     6961 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     7021 gggagggacg taattacatc cctgggggct ttgggggggg gctgtccccg tgagctccca
     7081 ggcgcgccaa ctcggtaccg tatcgatgtc gacgttaacg ctagtgatat caactttgta
     7141 tagaaaagtt ggctccgaat tcgcccttga cagcagagat ccagtttggt taattaattg
     7201 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttgtggg
     7261 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     7321 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     7381 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     7441 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     7501 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     7561 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     7621 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     7681 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     7741 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     7801 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     7861 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     7921 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     7981 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     8041 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     8101 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     8161 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     8221 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     8281 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     8341 tggttcaaag tttttttctt ccatttcagg tgtcgtgaga agggcgaatt cgacccaagt
     8401 ttgtacaaaa aagcaggctg accgccacca tggtgagcaa gggcgaggag ctgttcaccg
     8461 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtga
     8521 ggggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca
     8581 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtgcagt
     8641 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     8701 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcacctac aagacccgcg
     8761 ccgaggtgaa gttcgagggc gacaccctag tgaaccgcat cgagctgaag ggcgtcgact
     8821 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac agccacaaca
     8881 tctatatcat ggccgtcaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     8941 acgtggagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     9001 acggccccgt gctgctgccc gacagccact acctgagcac ccagtccgtg ctgagcaaag
     9061 accccaacga gaagcgcgat cacatggtcc tgctggagtt ccgcaccgcc gccgggatca
     9121 ctctcggcat ggacgagctg tacaagtaat acccagcttt cttgtacaaa gtggtacgcg
     9181 tgaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     9241 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     9301 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     9361 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     9421 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     9481 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     9541 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     9601 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     9661 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     9721 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9781 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     9841 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     9901 tgtcgtgcca gcggatcgac agtactaagc ttggtctaat acccaatctc tcgtcttatc
     9961 cagatgtttt atacgcctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
    10021 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
    10081 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
    10141 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
    10201 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
    10261 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
    10321 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
    10381 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
    10441 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
    10501 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
    10561 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
    10621 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
    10681 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
    10741 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
    10801 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
    10861 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
    10921 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
    10981 cgtgagttac gcgtcgttcc actgagcgtc agaggtatca ctagtattac agaggtaagt
    11041 tataacagtc gcctaaccc
//
