LOCUS       sequence-34632-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     SGC Q4
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NFATC2IP
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             91..111
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(475..493)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      489..536
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(711..716,717..733,734..739))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(766..784)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gggaaattcc ctctgaatat tttgtttact ttaagaagga gatataccat gggcagcagc
       61 catcatcatc atcatcacag cagcggcaga gaaaacttgt atttccaggg ccagggccaa
      121 gaggatgaag tggtcttggt ggaagggccc accctcccag agaccccccg actcttccca
      181 ctcaaaatcc gttgccgggc tgacctggtc agattgcccc tcaggatgtc ggagcccctg
      241 cagagtgtgg tggaccacat ggccacccac cttggggtgt ccccaagcag gatccttttg
      301 ctttttggag agacagagct atcacctact gccactccca ggaccctaaa gctcggagtg
      361 gctgacatca ttgactgtgt ggtactaaca agttcttgat gacgaagctt gcggccgcac
      421 tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      481 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      541 aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
      601 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
      661 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
      721 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc
      781 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      841 ggggaaatgt gcgcggaaac cctatttgtt tatttttcta aatacattca a
//
