LOCUS       sequence-346338-        3326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 module containing NT2_TurboID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Velay F, Soula M, Mehrez M, Belbachir C, D'Alessandro S, Laloi C, 
            Crete P, Field B
  TITLE     MoBiFC: development of a modular bimolecular fluorescence 
            complementation toolkit for the analysis of chloroplast 
            protein-protein interactions.
  JOURNAL   Plant Methods. 2022 May 26;18(1):69. doi: 
            10.1186/s13007-022-00902-1.
  PUBMED    35619173
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2022-05-26"; volume: "18(1):69. doi"; pages: " 
            10.1186/s13007-022-00902-1"
COMMENT     Sequence Label: pLBC0150
FEATURES             Location/Qualifiers
     source          1..3326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(36..54)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        122..226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             448..1239
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1410..1998
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1899..1918
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2152..2169
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3228..3317
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
       61 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
      241 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
      301 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
      361 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
      421 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
      481 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
      541 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
      601 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
      661 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
      721 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
      781 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
      841 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
      901 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
      961 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     1021 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     1081 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     1141 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     1201 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     1261 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     1321 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1381 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1441 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1501 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1561 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1621 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1681 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1741 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1801 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1861 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1921 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1981 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2041 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2101 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2161 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2221 attcattaat cactctgtgg tctcaaatgg gaggttcggc ttctaaggat aatactgttc
     2281 ctttgaagtt gattgctttg ttggctaatg gagaatttca ttctggagaa caattgggag
     2341 aaactttggg aatgtctaga gctgctatta ataagcatat tcaaactttg agagattggg
     2401 gagttgatgt ttttactgtt cctggaaagg gatattcttt gcctgaacct attcctttgt
     2461 tgaatgctaa gcaaattttg ggacaattgg atggaggatc tgttgctgtt ttgcctgttg
     2521 ttgattctac taatcaatat ttgttggata gaattggaga attgaagtct ggagatgctt
     2581 gtattgctga atatcaacaa gctggaagag gatctagagg aagaaagtgg ttttctcctt
     2641 ttggagctaa tttgtatttg tctatgtttt ggagattgaa gagaggacct gctgctattg
     2701 gattgggacc tgttattgga attgttatgg ctgaagcttt gagaaagttg ggagctgata
     2761 aggttagagt taagtggcct aatgatttgt atttgcaaga tagaaagttg gctggaattt
     2821 tggttgaatt ggctggaatt actggagatg ctgctcaaat tgttattgga gctggaatta
     2881 atgttgctat gagaagagtt gaagaatctg ttgttaatca aggatggatt actttgcaag
     2941 aagctggaat taatttggat agaaatactt tggctgctac tttgattaga gaattgagag
     3001 ctgctttgga attgtttgaa caagaaggat tggctcctta tttgcctaga tgggaaaagt
     3061 tggataattt tattaataga cctgttaagt tgattattgg agataaggaa atttttggaa
     3121 tttctagagg aattgataag caaggagctt tgttgttgga acaagatgga gttattaagc
     3181 cttggatggg aggagaaatt tctttgagat ctgctgaaaa gttgcaatac ccatacgatg
     3241 ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggatcc tatccatatg
     3301 acgttccaga ttacgctgct ggaggt
//
