LOCUS       sequence-346380-       10714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10714)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3123
FEATURES             Location/Qualifiers
     source          1..10714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3468..3492)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3500..3541
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3652..4240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3705..3725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4123..4134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4312..4545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4623..4744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4660..4679)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4714..4733
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4784..4919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4939..4958)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4940..4958)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4968..4985)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4968..4984)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4977..4999)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5126..5581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5213..5232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5423..5444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5607..5711
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5712..5780,5781..6572)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5930..5949)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6743..7331
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7232..7251
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7485..7502
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7619..7640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7655..7660,7661..7678,7679..7685)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7693..7709
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7698..7720
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7717..7733
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7717..7733
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7752..7772
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7754..7772
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7800..8026
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8027..8207
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8254..8379
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8872..9105
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9290..9334
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9565..9775
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9587..9783
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9634..9769
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9815..10417
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9815..9834)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10311..10331
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10460..10577
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtctca gtggaatcaa gtccaacagt tagaaatcaa
     1261 gtttttggag caggtggatc aattctatga tgacaacttt cccatggaaa ttcggcatct
     1321 gttggcccaa tggattgaaa atcaagactg ggaggcagct tctaacaatg aaaccatggc
     1381 aacgattctt cttcaaaact tgttaataca actggatgaa cagttaggtc gtgtttccaa
     1441 agagaaaaac ctactcttga tacacaatct aaaaagaatt aggaaggtcc ttcagggaaa
     1501 atttcatgga aatccaatgc atgtagctgt ggttatttca aactgtttaa gggaagagag
     1561 gagaatattg gctgcagcca acatgcctgt ccaggggcct ctagagaaat ccttacaaag
     1621 ttcttcagtt tcagaaagac agaggaatgt ggagcacaaa gtggctgcca ttaaaaacag
     1681 tgtgcagatg acagaacaag ataccaaata cttagaagat ctgcaagacg aatttgacta
     1741 caggtataaa acaattcaga caatggatca gagtgacaag aatagtgcca tggtgaatca
     1801 ggaagttttg acactgcagg aaatgcttaa cagcctcgat ttcaagagaa aggaggctct
     1861 cagtaaaatg acccaaatca tccatgagac agacctgtta atgaacacca tgctcataga
     1921 agagctgcaa gactggaagc ggcggcagca aatcgcctgc atcgggggtc cactccacaa
     1981 tgggctcgac cagcttcaga actgctttac actattggca gaaagtcttt tccaactgag
     2041 aaggcaattg gagaaactag aggagcaatc taccaaaatg acatatgaag gtgatcccat
     2101 tccaatgcaa agaactcaca tgctagaaag agtcaccttc ttgatctaca accttttcaa
     2161 gaactcattt gtggttgagc gacagccatg tatgccaacc caccctcaga ggccgttggt
     2221 acttaaaacc ctaattcagt tcactgtaaa actaaggcta ctaataaaat tgccagaact
     2281 aaactatcag gtaaaggtta aggcatcaat tgacaagaat gtttcaactc taagcaaccg
     2341 aagatttgta ctttgtggaa ctaatgtcaa agccatgtct attgaagaat cttccaatgg
     2401 gagtctctca gtagaatttc gacatttgca accaaaggaa atgaagtcca gtgctggagg
     2461 taaaggaaat gagggctgtc acatggtgac tgaagaactt cattccataa cgtttgaaac
     2521 acagatctgc ctctatggcc tgaccataga tttggagacc agctcattgc ctgtggtgat
     2581 gatttccaat gtcagtcagt tacctaatgc ttgggcatcc atcatttggt acaacgtgtc
     2641 aaccaacgat tcccagaact tggttttctt taataatcct ccacctgcca cattgagtca
     2701 actactggag gtgatgagct ggcagttttc atcgtacgtt ggtcgtggtc ttaactcaga
     2761 tcaactccat atgctggcag agaagcttac agtccaatct agctacagtg atggtcacct
     2821 cacctgggcc aagttctgca aggaacattt acctggtaaa tcatttacct tttggacatg
     2881 gcttgaagca atattggatc taattaagaa acacattctt cccctttgga ttgatgggta
     2941 tgtcatgggc tttgttagca aagagaagga acggctgttg ctaaaggata aaatgcctgg
     3001 caccttttta ttaagattca gtgaaagcca tctcggagga ataactttca cctgggtgga
     3061 ccattctgaa agtggggaag tgagattcca ctctgtagaa ccctacaata aaggccggtt
     3121 gtctgctctg ccattcgctg acatcctgcg agactacaaa gttattatgg ctgaaaacat
     3181 tcctgaaaac cctctgaagt acctatatcc tgacattccc aaagacaaag ccttcggtaa
     3241 acactacagc tctcagcctt gcgaagtttc aagaccaaca gaaaggggtg acaaaggtta
     3301 tgttccttct gtttttatcc ccatctcaac aatccgaagt gattcaacag agccacattc
     3361 tccatcagac cttcttccca tgtctccaag tgtgtatgcg gtgttgagag aaaacctgag
     3421 tcccacaaca attgaaactg caatgaagtc tccttattct gctgaatgcc caactttctt
     3481 gtacaaagtg gttgatatcg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     3541 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     3601 tggaaaggac gaagatcctc agttgcccgg atggttacgc gttaagtcga caatcaacct
     3661 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3721 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3781 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3841 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3901 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3961 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4021 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4081 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4141 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4201 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     4261 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     4321 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     4381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     4561 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4621 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4681 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4741 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4861 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4921 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4981 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     5041 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     5101 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     5161 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     5221 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5281 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5341 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5401 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5461 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5521 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5581 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5641 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5701 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5761 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5821 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5881 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5941 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6001 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6061 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6121 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6181 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6241 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6301 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6361 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6421 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6481 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6541 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6601 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6661 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6721 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6781 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6841 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6901 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6961 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7021 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7081 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7141 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7201 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7261 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7321 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7381 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7441 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7501 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7561 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7621 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7681 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7741 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7801 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7861 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7921 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7981 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     8041 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     8101 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     8161 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     8221 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     8281 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     8341 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     8401 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8461 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8521 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8581 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8641 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8701 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8761 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8821 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8881 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8941 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9001 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9061 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9121 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9181 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9241 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9301 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9361 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9421 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9481 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     9541 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     9601 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9661 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9721 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9781 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     9841 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9901 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9961 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
    10021 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
    10081 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
    10141 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
    10201 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
    10261 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
    10321 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
    10381 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
    10441 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    10501 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    10561 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    10621 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
    10681 atggaccttc taggtcttga aaggagtggg aatt
//
