LOCUS       sequence-346381-       13763 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes sgRNA target 2300 and Gentamicin resistance - violacein 
            pathway transposon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13763)
  AUTHORS   Trujillo Rodriguez L, Ellington AJ, Reisch CR, Chevrette MG
  TITLE     CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse 
            Proteobacteria.
  JOURNAL   ACS Synth Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065.
  PUBMED    37368499
REFERENCE   2  (bases 1 to 13763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065."
COMMENT     Sequence Label: pUCSh5vio-2300
FEATURES             Location/Qualifiers
     source          1..13763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             110..643
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     protein_bind    883..930
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     terminator      complement(1364..1391)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        complement(1646..1680)
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
     terminator      complement(1694..1765)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1810..1914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1915..1983,1984..2775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2133..2152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2946..3534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3435..3454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3688..3705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      4070..4164
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      4267..4353
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    4401..4448
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     4483..4500
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4484..4500
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        5649..5698
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             5743..5754
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             9194..9202
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             13191..13208
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(13261..13279)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      13275..13322
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        join(13656..13661,13662..13678,13679..13684)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc atccaagcag
       61 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
      121 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat
      181 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct
      241 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc
      301 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca
      361 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag
      421 tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac
      481 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt
      541 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga
      601 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga
      661 gatcggcttc ccggccgcgg agttgttcgg taaattgtca caacgccgca ggtggcactt
      721 ttcggggagt ccagatagcc cagtagctga cattcatccg gggtctgcac cgtttctgcg
      781 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccatgca tttgttaaat
      841 cgaattatcc attgctgttg acaaagggaa tcaggggatc ttgaagttcc tattccgaag
      901 ttcctattct ctagaaagta taggaacttc agagcgcttt tgaagctaat tcgagatcat
      961 gcatgagctc actagtggat ccccctcagt aactattaaa cttaggggtg ggttgaaagc
     1021 aagtcctttt atccgcttgt tttaattgct ttgtataata attgcagagc atattatatt
     1081 gatgacattt aatttgtcat caattaatta agcaacgctg atgggtcacg acgacaatta
     1141 aatagtcaca atgacattaa tctgtcaccg acgacagata atttgtcact gtacactacg
     1201 ccttttgtgg agatgtctaa taaactaaaa attttttact ttccgctgca taaccctgct
     1261 tcggggtcat tgacgaaagg gcctcgtgat ctcaggccgc ctaggccggt ttcccgactg
     1321 gaaagcgggg ttccgcgcac atttccccga aaagtgccac ctgcgcagaa aggcccaccc
     1381 gaaggtgagc cctgcagtgc ttcgcccgct accccctttc aacccattta gggttccccc
     1441 ttgatcgggg gtgattcagt agccactaca gcagtacatc cgcgccctta ttgctgggag
     1501 cgcacctatc ctgtctattg acagcagcat cagggtgagc agctaccagg gtcagaaagc
     1561 aagactgtat ttatacagtc aaactaaccc tcatttaaca aaattcagag gctgcaagca
     1621 gcatgaattg cggcgctatt aatatgctag cattatacct aggactgagc tagctgtcag
     1681 ggtctctcta gtagagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     1741 tcgactgagc ctttcgtttt atttgatgcc tggagatcct taccaggtgg cacttttcgg
     1801 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1861 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1921 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1981 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2041 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2101 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2161 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2221 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2281 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2341 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2401 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2461 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2521 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2581 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2641 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2701 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2761 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3061 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3601 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3661 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggacagggaa
     3721 gagcgcaccg gctggtaaca ggattagcag agcgaggtat gtaggcggtg ctacaagatt
     3781 gtactgagag tgcaccatac aaaaccattg tagagaggta gcattgctac gtctctacgt
     3841 gtacagtgac taattatatg tcgttgtgac aaattattgt catcagtaaa atccttatac
     3901 agtatagatt atagcgcttt ggcagtttta gcataacctc tttgcagtga caaaatagat
     3961 gtcgttgtcc gtgattgtga caaattagct gtcgctttgc aagataggaa aaagcttttg
     4021 tgtattttca taatgacaaa ttgactgtcg ccttaattag ctgagcttgg actcctgttg
     4081 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
     4141 cgggcgtttt ttattggtga gaatccaagc tagactgcga tgagtggcag ggcggggcgt
     4201 aattttttta aggcagttat tggtgccctt aaacgcctgg ggtaatgact ctctagcttg
     4261 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4321 ttgtcggtga acgctctcct gagtaggaca aatccgccgc taggagcttg cggcccggac
     4381 gatctcgaat tggggatctt gaagtaccta ttccgaagtt cctattctct agaaagtata
     4441 ggaacttcag agcgcttttg aagctgatgt gcgtcacgac gttgtaaaac gacggccagt
     4501 gactattacc aattacgtcg cgtcatgcgg atcctatagg gattgatgat gcgcaattga
     4561 acagcccgcg acgcggcggc cgagatcgtg gtgcagccga gcctgaagcg ggcggttttc
     4621 aggtaatcgc gtgtggtatg ctctgatatg cccaggatga ccgaaatatc cttgtaatcc
     4681 ttgccgaggg cggtccagtg cagacactcg atctcgcgcg gcgacaatgc cggcaccgga
     4741 tcgttttcgc catgcagctc atatacggcc ttgcggtgga tcagatgggc gatctcgatc
     4801 cactcattgc ggcagcggcg cacgagctcg gtccattcgt cggccggtat atgggcattc
     4861 agcgacagca gggcgcggcg ctgcgccttg tcggcgacgg ggatggagta gccattgtca
     4921 tcgatgccgt gtttctgggc gtcgaccagc atggcatagg cctccggcgt cggttcgacc
     4981 tcgctccagt cgaagggcag ctggcgttcg aagccctgct tgatgatcgg atcgaccttc
     5041 acatagcagt tgaggaggta acgggaaacc caggcatccg gataggtggt gcgcacgaag
     5101 ggcgaatcga tcttgctcgc gattgtctgg gcgagatggt aggtgacgaa atcgaggtta
     5161 tattccgcct ggagaatacg gatggcggca tccacgttgg ccgccgcttt gatctgttcg
     5221 aacgcggact cgaacttttc gctataggta ttctcaatca ttcgccgttc tccgttgacg
     5281 ggcgtctcgg acctgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     5341 cgcgccgccc aattattgaa ggcctcccta acggggggcc tttttttgtt tctggtctcc
     5401 cgcttaacga tcgttggctg ccctttgtgc gtccaaacgg acgcacggcg ctctaaagcg
     5461 ggtcgcgatc tttcagattc gctcctcgcg ctttcagtct ttgttttggc gcatgtcgtt
     5521 atcgcaaaac cgctgcacac ttttgcgcga catgctctga tccccctcat ctgggggggc
     5581 ctatctgagg gaatttccga tccggctcgc ctgaaccatt ctgctttcca cgaacttgaa
     5641 aacgctagct gtcaccggat gtgctttccg gtctgatgag tccgtgagga cgaaacagcc
     5701 tctacaaata attttgttta agaactggga agagcgcaga ttaaagagga gaaatactag
     5761 atgacggtta gagaaaacac catctcaatc atcggagcgg gcatttcagg tattttttgc
     5821 gcgctaacgt tagcaaaatc aaaagagtgt aaaaatacaa ccattcgggt ttatgagcat
     5881 aaggatcgcg tgggtggtcg cgcacatgcc gtaaaagtca atgatcacta tgttgactta
     5941 ggtgctggac gcttttcttc agggcttcat cataatgtca aagagcaagt taagttgctt
     6001 gatttggcat atgaaaaatt cccttttaca caactaaaaa gaccgcaagc attgcataaa
     6061 agtttaaaga gcattctgaa aaatttgaaa ccactttgtg atgaacatcc ccacgattca
     6121 tttgaagagt ttttaactat ttacgtaggc agaatccaag caaaagaaat gatcaacgca
     6181 ttgggttatg actctttagc tctgccgaat attacgccac atatcgctta cgatataatt
     6241 gaaaaacacc ccgagattca ggggttttct gacaatgctg gctatgagtg gtttaacctg
     6301 aaggaaggct tttctgcact gaccgagaag ctctatgagc aggctctagc acacggtgtt
     6361 gagtttcatt ttggacatga gctgatcagt ctagaagctg atactgcgtc cccttcactc
     6421 atattgaaaa aagacaatca gcaaacagtg atcatagacg aaggtgaaat gattttaact
     6481 tttcctccaa cagctatgaa aaagctagat tgtggttttc cacaagattg gagcaactat
     6541 acctatggtt caattccgtt gttcaaaggc ttcttgtttt acgatgcacc ttggtggcaa
     6601 gagctcgact tagccgatca tgttgtcatt gccaacagcc cgatgcgcaa gctgtatttt
     6661 aaggacaata gatatgtatt tttttatacc gatagtgaat atgcgaactt ttggcgtgac
     6721 gccacattaa aaggtgaagc acattactta caaacggtaa aagaacttat ggcagaggcg
     6781 ctaaagtgtc atgtaagcca actcccagat cccaacaatc acacatacaa acactgggtc
     6841 catggtgttg agttctccca agaatcttgc ccaaatcacc ctgaaacact tgaacacaaa
     6901 cggtctcata tcatctcagc atcagatgct tatacatctc attgtggttg gatggagggc
     6961 ggcatcatgg caggaacgag tgctgcaaaa acattgctta cacgaattaa gcaatctcaa
     7021 gcaaaggctg taacaaagag tgagcttgta cacgtttaac tgtcatttga taaagacata
     7081 ctgtaaacaa agtaactagg aagttttaaa taaatgagta ttctcgattt tccccgagtt
     7141 cattttaaag gggttgcgcg tgtcaatgtg ccaacaggta atcgtaatat taacaatacc
     7201 ttagatatat cgacaaacac ggcgatcaag gatggtaaac cctacgacgt agcaaagcca
     7261 ccgcaggcct ttcatgacta cgtaaagtca ttcgggccaa aatttaatgc acacggccgt
     7321 cttgacgatg aaggtgaatt taacctagct gctggttaca acatgtgtgg taataaccac
     7381 ttcagctggg aaaacacctt aattacatcg gttcaactaa aacctggtga atacattacc
     7441 gaagataagt tagtcggcaa taaactgtca ctttgggggc attacaacga atatttgaga
     7501 acatcattta atcgtgcacg ctgggtcgac aacgacccga cacgaagaga ttcggcactc
     7561 atttatgctg gtcaactgac tatcacagac cctaaatcgt cagccaatac tgcacatatt
     7621 ttatcagcag acattgactg cacacatggt gttcgatggg ttaaccgtgg ttatattgaa
     7681 gagccgatta accactttct ccatgaagaa atgagcgaag ctcgtctgtt tcagttcaca
     7741 gtaaaaaaag acagtgaaga atttatattt aatcagctag gtcttgagtc tgaatttcta
     7801 tcagtattga aagataagct aaaagaccca acggtgaaag gcttagtggt acagtactgt
     7861 gtaagtaacc tgtctccgcc tatgacacct aacaaacccg ttttttgcga cttacacggc
     7921 tccattggtt tgtggtacga cgatgacatg aaaacctctc caacaggacg aattttaagg
     7981 ccggataata gtgagcagta cgcgcctatt gcgctatcag ttaaagataa ctggctcagt
     8041 ctaaacagtg cgattagttt tcctcatcaa aattatgaac aagctattcc ggttcaaaac
     8101 ggcatgccac ctgcactaag tggcaaggtt aacttgggtg atctgtactt gaaatccaat
     8161 agtggtcagg tgttagcagt gatacaaccg caagactaca taaacaatca cctcaatgct
     8221 ggtgtcattg atattccctt gcctgatatc gatgcacaat ttcatcagca atctttaaag
     8281 ctatgctcag agcggcacac ttggtacgaa agtgattggt atattcaagc tgagcaacac
     8341 atcctctcta tggaaagtcc aaacaaggag ctaaataagc caagtacaca gtcactggac
     8401 atacgcagct actacagagg tgagcctgca tctgtcagtc aattggccac ccacgtgtta
     8461 acacctaaca cagtggcttg ctctcctttt gttgaaacag atgacaatgg gcgaggaaaa
     8521 ctcgacattg agagccttgg tgcaggtagc gcagagctct ttttaggaga gttttatagt
     8581 ccagtgcaaa tccgtgtgct tgcagatgat tgggcattgg ctgatacgcc tacagagctg
     8641 gttgactatg actttctcta tacccatgtt atggcctatt acgaactttt ttatccattt
     8701 atgactgaag cggtattcag tatggcggat aagtgtaaat gtgaaaccta tgcccgacta
     8761 atgtggcaaa tgtgtgaccc aaaaaaccgc gacaaaagct attacatgcc gagcactcgg
     8821 gagatgccag aagtccactc acagctgttt ttaaagtatc tgtgtaatgt agaacaatcc
     8881 gctatgccaa atatattgcc agcagcgcaa tccgaggcaa agatccgcgg caaaatcaaa
     8941 aacaaaaagc agctgatcag cgcactaaaa gacgctgtgg acctagagct ttcaattatg
     9001 ctgcaatata tatacagcgc ctactcatta cctaactatg ctattggcga gcacttagtc
     9061 tcgcttggac gctggaacga gcaacaacta gaacttgtaa atggtggcaa agacagacga
     9121 ctcaatagcg gctggcgcgg tgcattactt gaaatcgcac acgaagaaat gatccactat
     9181 ttagtggtaa acaacctcct tatggcattg ggcgagccgt tttatccagg caagccggtt
     9241 ttgggagagc aagcaaaaga cacttttggt ctcgatactg agttttcatt cgaaccattt
     9301 tcagagcaca tagttgccaa atttgttcgc tttgagtggc cagcgttttt cccaaccgtc
     9361 gggaagtcta ttgcagactt ttatgccgat atcacaaaag caatcgatga actacctgac
     9421 ttatttggtg aagagaagat caatttgggc ggcgagcatc atttgtttct caatgaaatt
     9481 atcaatcgag aatatccagg ctatcagttt gaagtttacg ataaggagac agccttattc
     9541 gctattaact tcgtgactga gcaaggcgaa ggagttgctg ctgagtcccc ccaatttgaa
     9601 gtcagtcatt ttagtcgttt acgagcgata tcaaaagcct taactaacag tgatatccca
     9661 tttgaaccag catatccagt gctgaaaaat cccgtatttg aagcacgaag cgggtgtaac
     9721 ctcgtgacgg accccgcagc agtagaactt atgcagtttt ataaagggtg ccatgaatta
     9781 atgttccagc tgatgatgca acactttgct atcaagccgc tgggcagtat gcgccgttca
     9841 cgattaatga atggtgctat cgatctcatg accgggatat taaggccact ttcagtacta
     9901 cttatgacat taccttcagg cgtaccaggt agaaacgcgg gtccacctgt acctgaagct
     9961 attaacacac acgtatttcc agacctagag caagggtgta tggccatatc tgagcaatgt
    10021 aaaaagcttg cagctatggg tagagctatg gtctgtgcaa aacctccaac aacacaaatt
    10081 gagttattag agttttttca aaatcagatg catgaaatcg caactggcaa actttcaagg
    10141 gaagcttaat catgaagaaa ataattgtca ttggcggcgg tctagccggt ggcctgacag
    10201 ccatatactt agcaagacgc ggatacgatg tacacatcat agagaaacgt ggaaaccctc
    10261 tattgagcca agcagactac attgatcagg tcagctcacg cgccattggt gtaagtatga
    10321 ctgtgcgggg catagaggct gtggttgcgg cagggatccc gcttgaagag cttcaagcat
    10381 gcggtgtaga agtatctggc atgtcgtttt ttgttaaagg agagccaaaa acgcgtgaac
    10441 tacccccgct tgataaactc aagccgctat ctctcagtcg cagtgcgttt caagttttgt
    10501 taaacaaata tgcggaactt gcaggtgtga actatcacta tgatcaacgc tgtatcgaag
    10561 tcaatttaga taactgctca gtgatgacaa aagacagcaa tgacactttt gttactcata
    10621 aaggtgattt acttatcggt gcagatggcg cgcgttcgtg tgtcagagaa gcgatgcagt
    10681 cccattgtag gcgttttgag ttccaccaat catttttcaa acatggttat aaaacactgg
    10741 ttgtacccga tgcatctaaa gttggattgc gcaaagatct cctgtacttc tttggcatgg
    10801 actcagcagg acaatttgca gggcgtgctg cgacaatccc cgacggcagt atcagctttg
    10861 cggtttgcct tccttatacg ggtcaagtga gcttgcacag tgacgacagc accgcaatgc
    10921 gagcattctt cgacaaatac tacccaatgg ttcctgaaga tattcgtcaa gatatgtttg
    10981 agcaattcat gatcaagcct agcaatgact taatcaacgt gcgctcatct acgttccatt
    11041 ataaaaacac cgcattactg attggcgatt cagcccatgc gaccgcacca tttttaggtc
    11101 aagggatgaa tatggcgctc gaagacgcct atgtattgga caccttgttt gaccaatatg
    11161 gcgatgactt taccaagatc ctcgaagaat ttaccgttca gcgcaaaatt gaagccgatg
    11221 ccatgcagga tatggcccgt gctaactatg aagttcttag ttgctcagac cctatctttt
    11281 ttatgcgagc tcgatttgca cgctatatga atcagaaatt tccagatcat tacccgccag
    11341 atatggcaga aaaactttac ttcacatcaa tgcgttacag tgaattaaga gataagcaag
    11401 aaaagcaaaa cgtttggtac aaaataggga gaatgaatta atgaagattt tagttgttgg
    11461 tgccggtcca tcaggtctga tgtttgctag tgaaaccaaa aagttaaagc cagaatggga
    11521 aattcgtatc attgagaaaa accaccctga cgccaacgtc gttggctggg gtgttgtact
    11581 gccagggaga gcgccgcatc acccggctaa ccctttgtca tatttagaaa accatgaaat
    11641 actcgatgcc cagtatttag aagactttaa gctaacgcac cacaatcaaa gtgcgttgaa
    11701 aagcacagga gtgaccttgt gtggtgccga gcgaaagtcg atggttcgtg aacttagaca
    11761 attatgtgtc ggcttaggta tcaccataga ttacgaacat cctgcggtag aagatgcaga
    11821 gctattgtgc aacgaatacg atttagtcgt ggtttcaaac ggcattaatc acaccacgac
    11881 ttattaccgt gattccctca aacccaaagt ggaatttggt aaaaaccgct acatgtggta
    11941 cggcacacca aaaacatttg atgccatgaa cttgatcttt aagtcgcact caaaaggcat
    12001 ttttgtcgca cactcttata aatattcaag cacgatgagc acattcgtcg tcgagtgcag
    12061 tgaagaaacc tatcaaaaat cggggcttga tgaaatgtcg ccgcaagaag cagagaaatt
    12121 catcgcttct gtctttgaaa gtgagttaga cggcaatccg gttatggcgc aagaaggctt
    12181 aaaatggcgt aactttatga cgcttagcca tgagaaagca tatgacggta acgttgctct
    12241 gctcggtgac gcattgcagt cgggtcactt ttcaatcggt cacggtacga ccatggctgt
    12301 ggttggcgca caaatcttgg ttaaagccgt tttcgataac cccgacaatg ttgaagctgc
    12361 attggctgac tttaaccagc acgttatgcc aatcatgcgc ctatttgatc aacatgcaac
    12421 catcagccgc ctttggtttg aaacggtaga agacagaatg cacttgtcta cgggcgaagt
    12481 cgctcagagc tttgctgagc gccgtggcga gctcccacca ttaccggcag ctttaggcca
    12541 agcgcttgaa agagcccttg cccgcggaga caaataatat ggatctgcaa aaacaaaagc
    12601 atccccctaa gctacctacg cagtggaata gcagttacat ttcatactgg cagccaatgc
    12661 agccgcaaga ccatatcacg tctggttatt gctggtttga ttacgccaat aatgtctgtc
    12721 gaatagatgg gttgtttaac ccttggtcgg aagaaaagac cggtcataga ttgtggatgt
    12781 cggagatcat gtacccggca accaatgaat cttttaagtc caaaatcgct tatagccgtg
    12841 cgcaaatgga tcaacaaagc tcgtttcaag agcaagtatt aaacgatgag gttgacccct
    12901 gccatgagtt gatattaatg caagatgtac ttgagctatg caacgccgag tttataggca
    12961 cctgtgaagt gatgggcact gaagcggata tttggcactt tgagcgtcct aataataagg
    13021 gaccagccac ttactatttc aaagcggata ccaatcaact tttgcgaatg gttacaggcg
    13081 atccacaaaa aatggcatcg gtacgggatt tccccaattt taatactcga gaaattgacc
    13141 ctgaaatttt tcaaccgact ccattaaaac aacctgagta agcactcgag caccaccacc
    13201 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
    13261 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
    13321 tgctgaaagg aggaactata tccggattgg cgaatgggag ctttccagtc gggaaacctg
    13381 tcgtgaggct aggtggaggc tcagtgagct ttccagtcgg gaaacctgtc gtgccagctg
    13441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg catgcataaa
    13501 aactgttgta attcattaag cattctgccg acatggaatc catcacaaac ggcatgatga
    13561 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggac
    13621 gcacaccgtg gaaacggatg aaggcacgaa cccagttgac ataagcctgt tcggttcgta
    13681 aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
    13741 gcggtggtaa cggcgcagtg gcg
//
