LOCUS       sequence-346385-       10285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10285)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3125
FEATURES             Location/Qualifiers
     source          1..10285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3039..3063)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3071..3112
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3223..3811
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3276..3296)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3694..3705)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3883..4116
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4194..4315
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4231..4250)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4285..4304
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4355..4490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4510..4529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4511..4529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4539..4556)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4539..4555)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4548..4570)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4697..5152
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4784..4803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4994..5015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5178..5282
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5283..5351,5352..6143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5501..5520)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6314..6902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6803..6822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7056..7073
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7190..7211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7226..7231,7232..7249,7250..7256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7264..7280
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7269..7291
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7288..7304
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7288..7304
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7323..7343
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7325..7343
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7371..7597
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7598..7778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7825..7950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8443..8676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8861..8905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9136..9346
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9158..9354
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9205..9340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9386..9988
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9386..9405)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9882..9902
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10031..10148
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgat gaagtcccag gggttagtat cattcaagga
     1261 tgtggctgtg gatttcaccc aggaggagtg gcagcaactt gacccttctc agaggaccct
     1321 gtacagggat gtgatgctgg agaactacag ccacctggtc tcaatggggt atccagtttc
     1381 caaaccagat gtcatctcca agttggaaca aggagaagag ccatggatca taaagggaga
     1441 catatcaaat tggatctatc cagatgaata tcaggcagat gggagacaag acaggaagag
     1501 taaccttcac aactcccagt catgtatttt ggggacagtt tccttccatc ataagatact
     1561 gaaaggagtc acaagggatg gttcattgtg ctccatttta aaagtctgtc aaggtgatgg
     1621 tcagctgcag agatttctag agaatcaaga caaactcttc aggcaggtca catttgttaa
     1681 cagcaaaaca gtgactgagg catcagggca taaatataat ccactgggga aaatatttca
     1741 agagtgcata gaaacagata tatcaataca gagattccat aaatatgatg cttttaaaaa
     1801 gaacttaaaa ccaaatattg acctaccgag ttgttataag agcaattcaa gaaaaaaacc
     1861 tgatcagagt tttggaggtg gaaaatcatc tagccagagt gagcccaatt ctaatcttga
     1921 gaagattcac aatggagtaa taccttttga tgataatcag tgtggaaacg tttttagaaa
     1981 tacacaatcc cttattcaat atcagaatgt ggaaactaaa gagaaaagct gtgtatgtgt
     2041 tacatgtgga aaagcctttg ctaagaagtc acaactcatt gtacatcaaa gaattcatac
     2101 tggaaagaaa ccatatgatt gtggtgcatg cggaaaagcc ttcagtgaga agtttcatct
     2161 tgttgtacat cagagaactc atactgggga gaaaccttat gattgttctg aatgtggaaa
     2221 agccttctct cagaaatcgt cccttattat acatcagaga gttcacactg gggaaaaacc
     2281 ctatgaatgt agtgaatgcg ggaaagcctt ctcccagaaa tcacccctca ttatacatca
     2341 gagaatacat actggggaaa aaccctatga atgtagagag tgtgggaagg ccttttccca
     2401 gaagtcacag ctgattatac accacagagc tcatactgga gagaagccgt atgagtgtac
     2461 cgaatgtggg aaagccttct gtgagaagtc ccacctcatt atacataaaa gaattcacac
     2521 tggtgagaaa ccctacaaat gtgctcaatg tgaggaagcc ttcagcagga agacagaact
     2581 cattacacat cagttagttc atactgggga aaaaccttat gaatgtactg aatgtggaaa
     2641 gacattctcc cgcaagtcac agctcatcat acatcagaga acacatactg gagaaaaacc
     2701 ctataaatgt agtgaatgtg gcaaagcctt ctgccagaag tcacatctca ttggacatca
     2761 gagaattcac acaggagaaa aaccttatat atgtactgaa tgtgggaaag ccttctctca
     2821 gaagtcccac cttccgggac accagcgaat tcatacagga gagaaacctt acatatgtgc
     2881 tgaatgtgga aaggcctttt ctcagaagtc agaccttgtt ttacatcaga ggattcatac
     2941 tggggaaaga ccctatcaat gtgctatatg tgggaaggcc ttcatccaga agtcacaact
     3001 aactgtacac cagagaattc acacagtggt aaaatcattg ccaactttct tgtacaaagt
     3061 ggttgatatc ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg
     3121 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     3181 cgaaagagga tgtggtagcc attttccacg cgttaagtcg acaatcaacc tctggattac
     3241 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3301 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3361 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3421 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3481 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3541 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3601 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     3661 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3721 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3781 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     3841 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3901 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3961 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4021 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4081 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     4141 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     4201 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4261 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4321 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4381 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4441 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     4501 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     4561 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4621 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4681 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4741 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4801 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4861 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4921 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4981 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5041 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5101 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     5161 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5221 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5281 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5341 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5401 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5461 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5521 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5581 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5641 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5701 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5761 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5821 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5881 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5941 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6001 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6061 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6121 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6181 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6241 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6301 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6361 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6421 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6481 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6541 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6601 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6661 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6721 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6781 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6841 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6901 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6961 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7021 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7081 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7141 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7201 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7261 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7321 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     7381 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     7441 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     7501 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     7561 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     7621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     7801 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     7861 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     7921 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     7981 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8041 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8101 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8161 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8221 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8281 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8341 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8401 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8461 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8521 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8581 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8641 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8701 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8761 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8821 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     8881 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     8941 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9001 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9061 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     9121 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     9181 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9241 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9301 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     9361 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     9421 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     9481 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     9541 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     9601 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     9661 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     9721 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     9781 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     9841 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     9901 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     9961 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    10021 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    10081 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    10141 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    10201 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    10261 ctaggtcttg aaaggagtgg gaatt
//
