LOCUS       sequence-346387-        9148 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 plasmid to create GFP fusion proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9148)
  TITLE     ENCODE collection: CRISPR constructs
REFERENCE   2  (bases 1 to 9148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCHCHD3-N2.1.2-gDNA
FEATURES             Location/Qualifiers
     source          1..9148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        118..403
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     misc_RNA        complement(500..575)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(597..845)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(655..674)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(825..845)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(907..1495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(987..1006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1666..2457,2458..2526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2289..2308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2527..2631)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2699..2717
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2755..2777)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2877..2896
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2913..3368)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3050..3071)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3262..3281
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3443..3583
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3454..3583
                     /label=AAV2 ITR
     polyA_signal    3592..3799
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3788..3805
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3833..4432)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3916..3936)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4413..4432
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(4433..4486)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4502..4549)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(4550..8623,8624..8626,8627..8650))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4550..8650)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8675..8695)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8702..8767)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      8767..8776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8788..9015
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
ORIGIN
        1 cccccccccc gcccccatcg ctgcacaaaa taattaaaaa ataaataaat acaaaattgg
       61 gggtggggag gggggggaga tggggagagt gaagcagaac gtggggctca cctcgaccat
      121 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
      181 gtactgggca caatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
      241 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
      301 ttgacgtcaa tggaaagtcc ctattggcgt tactattgac gtcaatgggc gggggtcgtt
      361 gggcggtcag ccaggcgggc catttaccgt aagttatgta acgggtacct ctagagccat
      421 ttgtctgcag aattggcgca cgcgctaaaa acggactagc cttattttaa cttgctattt
      481 ctagctctaa aacaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta
      541 gccttatttt aacttgctat ttctagctct aaaacagtga gaaaacaacc catttcggtg
      601 tttcgtcctt tccacaagat atataaagcc aagaaatcga aatactttca agttacggta
      661 agcatatgat agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat
      721 tactttctac gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcca
      781 attatctctc taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg
      841 ccctcacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1801 accgcggctt ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2641 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2701 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     2761 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     2821 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     2881 atcagagcag attgtactga gagtgcacca taaaattgta aacgttaata ttttgttaaa
     2941 attcgcgtta aatttttgtt aaatcagctc attttttaac caatagaccg aaatcggcaa
     3001 aatcccttat aaatcaaaag aatagcccga gatagagttg agtgttgttc cagtttggaa
     3061 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     3121 gggcgatggc ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg
     3181 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     3241 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta aggcgctggc
     3301 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     3361 gggcgcgtac tatggttgct ttgacgtatg cggtgtgaaa taccgcacag atgcgtaagg
     3421 agaaaatacc gcatcaggcg cccctgcagg cagctgcgcg ctcgctcgct cactgaggcc
     3481 gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga
     3541 gcgcgcagag agggagtggc caactccatc actaggggtt cctgcggccg ctccccagca
     3601 tgcctgctat tctcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     3661 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     3721 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     3781 tggcaactag aaggcacagt cgaggctgat cagcgagctc tagttagaat tctcaggcac
     3841 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
     3901 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
     3961 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
     4021 ccttgccctg gtggtcgggc gagactccga cggtggccag gaaccacgcg ggctccttgg
     4081 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
     4141 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
     4201 tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
     4261 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat
     4321 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
     4381 tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc attgggccag
     4441 gattctcctc gacgtcaccg catgttagca gacttcctct gccctctcca ctgccgaatt
     4501 cctttttctt ttttgcctgg ccggcctttt tcgtggccgc cggccttttg tcgcctccca
     4561 gctgagacag gtcgatccgt gtctcgtaca ggccggtgat gctctggtgg atcagggtgg
     4621 cgtccagcac ctctttggtg ctggtgtacc tcttccggtc gatggtggtg tcaaagtact
     4681 tgaaggcggc aggggctccc agattggtca gggtaaacag gtggatgata ttctcggcct
     4741 gctctctgat gggcttatcc cggtgcttgt tgtaggcgga cagcactttg tccagattag
     4801 cgtcggccag gatcactctc ttggagaact cgctgatctg ctcgatgatc tcgtccaggt
     4861 agtgcttgtg ctgttccaca aacagctgtt tctgctcatt atcctcgggg gagcccttca
     4921 gcttctcata gtggctggcc aggtacagga agttcacata tttggagggc agggccagtt
     4981 cgtttccctt ctgcagttcg ccggcagagg ccagcattct cttccggccg ttttccagct
     5041 cgaacaggga gtacttaggc agcttgatga tcaggtcctt tttcacttct ttgtagccct
     5101 tggcttccag aaagtcgatg ggattcttct cgaagctgct tctttccatg atggtgatcc
     5161 ccagcagctc tttcacactc ttcagtttct tggacttgcc cttttccact ttggccacca
     5221 ccagcacaga ataggccacg gtggggctgt cgaagccgcc gtacttctta gggtcccagt
     5281 ccttctttct ggcgatcagc ttatcgctgt tcctcttggg caggatagac tctttgctga
     5341 agccgcctgt ctgcacctcg gtctttttca cgatattcac ttggggcatg ctcagcactt
     5401 tccgcacggt ggcaaaatcc cggcccttat cccacacgat ctccccggtt tcgccgtttg
     5461 tctcgatcag aggccgcttc cggatctcgc cgttggccag ggtaatctcg gtcttgaaaa
     5521 agttcatgat gttgctgtag aagaagtact tggcggtagc cttgccgatt tcctgctcgc
     5581 tcttggcgat catcttccgc acgtcgtaca ccttgtagtc gccgtacacg aactcgcttt
     5641 ccagcttagg gtactttttg atcagggcgg ttcccacgac ggcgttcagg taggcgtcgt
     5701 gggcgtggtg gtagttgttg atctcgcgca ctttgtaaaa ctggaaatcc ttccggaaat
     5761 cggacaccag cttggacttc agggtgatca ctttcacttc ccggatcagc ttgtcattct
     5821 cgtcgtactt agtgttcatc cgggagtcca ggatctgtgc cacgtgcttt gtgatctgcc
     5881 gggtttccac cagctgtctc ttgatgaagc cggccttatc cagttcgctc aggccgcctc
     5941 tctcggcctt ggtcagattg tcgaactttc tctgggtaat cagcttggcg ttcagcagct
     6001 gccgccagta gttcttcatc ttcttcacga cctcttcgga gggcacgttg tcgctcttgc
     6061 cccggttctt gtcgcttctg gtcagcacct tgttgtcgat ggagtcgtcc ttcagaaagc
     6121 tctgaggcac gatatggtcc acatcgtagt cggacagccg gttgatgtcc agttcctggt
     6181 ccacgtacat atcccgccca ttctgcaggt agtacaggta cagcttctcg ttctgcagct
     6241 gggtgttttc cacggggtgt tctttcagga tctggctgcc cagctctttg atgccctctt
     6301 cgatccgctt cattctctcg cggctgttct tctgtccctt ctgggtggtc tggttctctc
     6361 tggccatttc gatcacgatg ttctcgggct tgtgccggcc catcactttc acgagctcgt
     6421 ccaccacctt cactgtctgc aggatgccct tcttaatggc ggggctgccg gccagattgg
     6481 caatgtgctc gtgcaggcta tcgccctggc cggacacctg ggctttctgg atgtcctctt
     6541 taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt tctgttggcg aagccgtcgg
     6601 acttcaggaa atccaggatt gtcttgccgg actgcttgtc ccggatgccg ttgatcagct
     6661 tccggctcag cctgccccag ccggtgtatc tccgccgctt cagctgcttc atcactttgt
     6721 cgtcgaacag gtgggcatag gttttcagcc gttcctcgat catctctctg tcctcaaaca
     6781 gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt ttcctcattg tccaggaagt
     6841 ccttgtcctt gataattttc agcagatcgt ggtatgtgcc cagggaggcg ttgaaccgat
     6901 cttccacgcc ggagatttcc acggagtcga agcactcgat tttcttgaag tagtcctctt
     6961 tcagctgctt cacggtcact ttccggttgg tcttgaacag caggtccacg atggcctttt
     7021 tctgctcgcc gctcaggaag gcgggctttc tcattccctc ggtcacgtat ttcactttgg
     7081 tcagctcgtt atacacggtg aagtactcgt acagcaggct gtgcttgggc agcaccttct
     7141 cgttgggcag gttcttatcg aagttggtca tccgctcgat gaagctctgg gcggaagcgc
     7201 ccttgtccac cacttcctcg aagttccagg gggtgatggt ttcctcgctc tttctggtca
     7261 tccaggcgaa tctgctgttt cccctggcca gagggcccac gtagtagggg atgcggaagg
     7321 tcaggatctt ctcgatcttt tcccggttgt ccttcaggaa tgggtaaaaa tcttcctgcc
     7381 gccgcagaat ggcgtgcagc tctcccaggt ggatctggtg ggggatgctg ccgttgtcga
     7441 aggtccgctg cttccgcagc aggtcctctc tgttcagctt cacgagcagt tcctcggtgc
     7501 cgtccatctt ttccaggatg ggcttgatga acttgtagaa ctcttcctgg ctggctccgc
     7561 cgtcaatgta gccggcgtag ccgttcttgc tctggtcgaa gaaaatctct ttgtacttct
     7621 caggcagctg ctgccgcacg agagctttca gcagggtcag gtcctggtgg tgctcgtcgt
     7681 atctcttgat catagaggcg ctcagggggg ccttggtgat ctcggtgttc actctcagga
     7741 tgtcgctcag caggatggcg tcggacaggt tcttggcggc cagaaacagg tcggcgtact
     7801 ggtcgccgat ctgggccagc aggttgtcca ggtcgtcgtc gtaggtgtcc ttgctcagct
     7861 gcagtttggc atcctcggcc aggtcgaagt tgctcttgaa gttgggggtc aggcccaggc
     7921 tcagggcaat caggttgccg aacaggccat tcttcttctc gccgggcagc tgggcgatca
     7981 gattttccag ccgtctgctc ttgctcagtc tggcagacag gatggccttg gcgtccacgc
     8041 cgctggcgtt gatggggttt tcctcgaaca gctggttgta ggtctgcacc agctggatga
     8101 acagcttgtc cacgtcgctg ttgtcggggt tcaggtcgcc ctcgatcagg aagtggcccc
     8161 ggaacttgat catgtgggcc agggccagat agatcagccg caggtcggcc ttgtcggtgc
     8221 tgtccaccag tttctttctc aggtggtaga tggtggggta cttctcgtgg taggccacct
     8281 cgtccacgat gttgccgaag atggggtgcc gctcgtgctt cttatcctct tccaccagga
     8341 aggactcttc cagtctgtgg aagaagctgt cgtccacctt ggccatctcg ttgctgaaga
     8401 tctcttgcag atagcagatc cggttcttcc gtctggtgta tcttcttctg gcggttctct
     8461 tcagccgggt ggcctcggct gtttcgccgc tgtcgaacag cagggctccg atcaggttct
     8521 tcttgatgct gtgccggtcg gtgttgccca gcaccttgaa tttcttgctg ggcaccttgt
     8581 actcgtcggt gatcacggcc cagcccacag agttggtgcc gatggccagg ccgatgctgt
     8641 acttcttgtc ggctgctggg actccgtgga taccgacctt ccgcttcttc tttggggcca
     8701 tcttatcgtc atcgtctttg taatcaatat catgatcctt gtagtctccg tcgtggtcct
     8761 tatagtccat ggtggcaccg gtccaacctg aaaaaaagtg atttcaggca ggtgctccag
     8821 gtaattaaac attaataccc caccaaccaa ccatccctta aacccttacc tcttgctcag
     8881 ctaattacag cccggaggag aagggccgtc ccgcccgctc acctgtggga gtaacgcggt
     8941 cagtcagagc cggggcgggc ggcgcgaggc ggcggcggag cggggcacgg ggcgaaggca
     9001 gcgcgcagcg actcccgccc gccgcgcgct tcgcttttta tagggccgcc gccgccgccg
     9061 cctcgccata aaaggaaact ttcggagcgc gccgctctga ttggctgccg ccgcacctct
     9121 ccgcctcgcc ccgccccgcc cctcgccc
//
