LOCUS       sequence-346435-        1319 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses KSHV ORF45 tagged, double stop.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Alzhanova D, Corcoran K, Bailey AG, Long K, Taft-Benz S, Graham RL, 
            Broussard GS, Heise M, Neumann G, Halfmann P, Kawaoka Y, Baric RS, 
            Damania B, Dittmer DP
  TITLE     Novel modulators of p53-signaling encoded by unknown genes of 
            emerging viruses.
  JOURNAL   PLoS Pathog. 2021 Jan 7;17(1):e1009033. doi: 
            10.1371/journal.ppat.1009033. eCollection 2021 Jan.
  PUBMED    33411764
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-01-7"; volume: "17(1):e1009033. doi"; pages: "
            10.1371/journal.ppat.1009033. eCollection 2021 Jan"
COMMENT     Sequence Label: pOME0016L
FEATURES             Location/Qualifiers
     source          1..1319
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1234..1299
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggatccgcca ccatggcgat gtttgtgagg acctcgtcta gcacacacga tgaagagaga
       61 atgcttccaa ttgaaggagc gcctcgcaga cgaccccccg tgaagttcat attcccacct
      121 ccacctcttt catcacttcc aggatttggc aggccgcgcg gctatgctgg acccacggtg
      181 atagatatgt ctgccccaga cgacgtcttc gccgaggaca cgccatcgcc gccagcaacc
      241 cctctggatc tacagatatc cccggatcag tcgagcggcg aatctgaata tgacgaggat
      301 gaggaagatg aagatgaaga agaaaatgac gatgttcagg aggaagacga gccagagggg
      361 taccctgcag acttttttca acctttatct cacttgcgcc cgaggcctct ggccagacgg
      421 gcccatacgc ccaaaccggt agcagtggta gcgggccgcg tgcgcagttc aacggacacg
      481 gcggagtccg aggcgtccat gggatgggtt agtcaggatg acggattttc ccctgctggg
      541 ctctcacctt cagacgacga gggggttgct atcctggaac cgatggcggc atacactggg
      601 accggggcat acggactttc acctgcttcc agaaatagtg tacctggaac acaaagttca
      661 ccatacagcg accctgatga agggccctcg tggcgccccc tgcgcgccgc acccaccgcg
      721 atcgtcgacc tgacatcgga ctctgatagc gatgacagtt ccaactctcc ggacgtgaac
      781 aatgaggccg cgtttaccga cgcgcgccat ttttcccacc agccaccctc gtccgaggag
      841 gacggagaag accaagggga agtattgagt cagagaatcg ggctcatgga cgtgggccag
      901 aagcgcaaaa ggcagtctac cgcctcctct ggtagcgagg atgtggtgcg ctgccagaga
      961 caaccaaact taagccgcaa agcagtggcg tccgtgataa ttatatcctc ggggagtgac
     1021 acagacgagg agccctcgtc cgccgtgagc gtgatcgtgt ctccgtcgag cacaaagggt
     1081 cacctcccaa cccaatctcc cagtacttcc gcccactcga tttcatcagg aagcacaact
     1141 accgcggggt ccaggtgcag cgacccaacc cgcatcctgg cctccacgcc acccctgtgt
     1201 ggaaacggtg catataactg gccgtggctg gacgactaca aagaccatga cggtgattat
     1261 aaagatcatg atatcgatta caaggatgac gatgacaagt agtaactcga ggcggccgc
//
